Limosilactobacillus reuteri DS0384 promotes intestinal epithelial maturation via the postbiotic effect in human intestinal organoids and infant mice
Little is known about the modulatory capacity of the microbiota in early intestinal development. We examined various intestinal models that respond to gut microbial metabolites based on human pluripotent stem cell-derived human intestinal organoids (hIOs): physiologically relevant in vitro fetal-lik...
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| Language: | English |
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Taylor & Francis Group
2022-12-01
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| Series: | Gut Microbes |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/19490976.2022.2121580 |
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| author | Hana Lee Kwang Bo Jung Ohman Kwon Ye Seul Son Eunho Choi Won Dong Yu Naeun Son Jun Hyoung Jeon Hana Jo Haneol Yang Yeong Rak Son Chan-Seok Yun Hyun-Soo Cho Sang Kyu Kim Dae-Soo Kim Doo-Sang Park Mi-Young Son |
| author_facet | Hana Lee Kwang Bo Jung Ohman Kwon Ye Seul Son Eunho Choi Won Dong Yu Naeun Son Jun Hyoung Jeon Hana Jo Haneol Yang Yeong Rak Son Chan-Seok Yun Hyun-Soo Cho Sang Kyu Kim Dae-Soo Kim Doo-Sang Park Mi-Young Son |
| author_sort | Hana Lee |
| collection | DOAJ |
| description | Little is known about the modulatory capacity of the microbiota in early intestinal development. We examined various intestinal models that respond to gut microbial metabolites based on human pluripotent stem cell-derived human intestinal organoids (hIOs): physiologically relevant in vitro fetal-like intestine, intestinal stem cell, and intestinal disease models. We found that a newly isolated Limosilactobacillus reuteri strain DS0384 accelerated maturation of the fetal intestine using 3D hIO with immature fetal characteristics. Comparative metabolomic profiling analysis revealed that the secreted metabolite N-carbamyl glutamic acid (NCG) is involved in the beneficial effect of DS0384 cell-free supernatants on the intestinal maturation of hIOs. Experiments in an intestinal stem cell spheroid model and hIO-based intestinal inflamed model revealed that the cell-free supernatant from DS0384 comprising NCG promoted intestinal stem cell proliferation and was important for intestinal protection against cytokine-induced intestinal epithelial injury. The probiotic properties of DS0384 were also evaluated, including acid and bile tolerance and ability to adhere to human intestinal cells. Seven-day oral administration of DS0384 and cell-free supernatant promoted the intestinal development of newborn mice. Moreover, NCG exerted a protective effect on experimental colitis in mice. These results suggest that DS0384 is a useful agent for probiotic applications and therapeutic treatment for disorders of early gut development and for preventing intestinal barrier dysfunction. |
| format | Article |
| id | doaj-art-bcedc8c3adae41f9bf2188ec7de1d48b |
| institution | DOAJ |
| issn | 1949-0976 1949-0984 |
| language | English |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Gut Microbes |
| spelling | doaj-art-bcedc8c3adae41f9bf2188ec7de1d48b2025-08-20T03:05:25ZengTaylor & Francis GroupGut Microbes1949-09761949-09842022-12-0114110.1080/19490976.2022.2121580Limosilactobacillus reuteri DS0384 promotes intestinal epithelial maturation via the postbiotic effect in human intestinal organoids and infant miceHana Lee0Kwang Bo Jung1Ohman Kwon2Ye Seul Son3Eunho Choi4Won Dong Yu5Naeun Son6Jun Hyoung Jeon7Hana Jo8Haneol Yang9Yeong Rak Son10Chan-Seok Yun11Hyun-Soo Cho12Sang Kyu Kim13Dae-Soo Kim14Doo-Sang Park15Mi-Young Son16Stem Cell Research Convergence Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of KoreaStem Cell Research Convergence Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of KoreaStem Cell Research Convergence Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of KoreaStem Cell Research Convergence Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of KoreaStem Cell Research Convergence Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of KoreaStem Cell Research Convergence Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of KoreaStem Cell Research Convergence Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of KoreaKorean Collection for Type Cultures, Biological Resource Center, KRIBB, Jeongeup, Republic of KoreaKorean Collection for Type Cultures, Biological Resource Center, KRIBB, Jeongeup, Republic of KoreaKorean Collection for Type Cultures, Biological Resource Center, KRIBB, Jeongeup, Republic of KoreaKorean Collection for Type Cultures, Biological Resource Center, KRIBB, Jeongeup, Republic of KoreaKorean Collection for Type Cultures, Biological Resource Center, KRIBB, Jeongeup, Republic of KoreaKRIBB School of Bioscience, Korea University of Science and Technology (UST), Daejeon, Republic of KoreaLaboratory of Efficacy Research, Korea Ginseng Corp., Daejeon, Republic of KoreaKRIBB School of Bioscience, Korea University of Science and Technology (UST), Daejeon, Republic of KoreaKorean Collection for Type Cultures, Biological Resource Center, KRIBB, Jeongeup, Republic of KoreaStem Cell Research Convergence Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of KoreaLittle is known about the modulatory capacity of the microbiota in early intestinal development. We examined various intestinal models that respond to gut microbial metabolites based on human pluripotent stem cell-derived human intestinal organoids (hIOs): physiologically relevant in vitro fetal-like intestine, intestinal stem cell, and intestinal disease models. We found that a newly isolated Limosilactobacillus reuteri strain DS0384 accelerated maturation of the fetal intestine using 3D hIO with immature fetal characteristics. Comparative metabolomic profiling analysis revealed that the secreted metabolite N-carbamyl glutamic acid (NCG) is involved in the beneficial effect of DS0384 cell-free supernatants on the intestinal maturation of hIOs. Experiments in an intestinal stem cell spheroid model and hIO-based intestinal inflamed model revealed that the cell-free supernatant from DS0384 comprising NCG promoted intestinal stem cell proliferation and was important for intestinal protection against cytokine-induced intestinal epithelial injury. The probiotic properties of DS0384 were also evaluated, including acid and bile tolerance and ability to adhere to human intestinal cells. Seven-day oral administration of DS0384 and cell-free supernatant promoted the intestinal development of newborn mice. Moreover, NCG exerted a protective effect on experimental colitis in mice. These results suggest that DS0384 is a useful agent for probiotic applications and therapeutic treatment for disorders of early gut development and for preventing intestinal barrier dysfunction.https://www.tandfonline.com/doi/10.1080/19490976.2022.2121580Human intestinal organoidintestinal developmentepithelial maturationgut microbiotaprobioticpostbiotic |
| spellingShingle | Hana Lee Kwang Bo Jung Ohman Kwon Ye Seul Son Eunho Choi Won Dong Yu Naeun Son Jun Hyoung Jeon Hana Jo Haneol Yang Yeong Rak Son Chan-Seok Yun Hyun-Soo Cho Sang Kyu Kim Dae-Soo Kim Doo-Sang Park Mi-Young Son Limosilactobacillus reuteri DS0384 promotes intestinal epithelial maturation via the postbiotic effect in human intestinal organoids and infant mice Gut Microbes Human intestinal organoid intestinal development epithelial maturation gut microbiota probiotic postbiotic |
| title | Limosilactobacillus reuteri DS0384 promotes intestinal epithelial maturation via the postbiotic effect in human intestinal organoids and infant mice |
| title_full | Limosilactobacillus reuteri DS0384 promotes intestinal epithelial maturation via the postbiotic effect in human intestinal organoids and infant mice |
| title_fullStr | Limosilactobacillus reuteri DS0384 promotes intestinal epithelial maturation via the postbiotic effect in human intestinal organoids and infant mice |
| title_full_unstemmed | Limosilactobacillus reuteri DS0384 promotes intestinal epithelial maturation via the postbiotic effect in human intestinal organoids and infant mice |
| title_short | Limosilactobacillus reuteri DS0384 promotes intestinal epithelial maturation via the postbiotic effect in human intestinal organoids and infant mice |
| title_sort | limosilactobacillus reuteri ds0384 promotes intestinal epithelial maturation via the postbiotic effect in human intestinal organoids and infant mice |
| topic | Human intestinal organoid intestinal development epithelial maturation gut microbiota probiotic postbiotic |
| url | https://www.tandfonline.com/doi/10.1080/19490976.2022.2121580 |
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