Sensitive Determination of Hydrochlorothiazide Drug in the Various Samples Via Developed Method of CFIA System

Hydrochlorothiazide drug determination in pharmaceutical and biological urine samples using a novel continuous flow-injection analysis technique that is rapid, simple, economical, and sensitive. The complex formed by the reaction of hydrochlorothiazide and O-phenylenediamine with potassium ferricya...

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Main Authors: Wasan A. Hanooan, Bushra B. Qassim
Format: Article
Language:English
Published: University of Baghdad, College of Science for Women 2024-09-01
Series:مجلة بغداد للعلوم
Subjects:
Online Access:https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/8460
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author Wasan A. Hanooan
Bushra B. Qassim
author_facet Wasan A. Hanooan
Bushra B. Qassim
author_sort Wasan A. Hanooan
collection DOAJ
description Hydrochlorothiazide drug determination in pharmaceutical and biological urine samples using a novel continuous flow-injection analysis technique that is rapid, simple, economical, and sensitive. The complex formed by the reaction of hydrochlorothiazide and O-phenylenediamine with potassium ferricyanide to produce an orange-colored product at 480 nm was the basis for the proposed method. Other chemical and physical parameters that affect the stability and development of the colored product used in this developed method includes sample volume, flow rate, reagent concentrations, and reaction coil. The proposed method’s linearity ranges from 5 to 150 μg.mL-1 and its correlation coefficients were 0.9988, and the recovery percentage and error percentage were 100.88 and 0.88, respectively. The detection limit and Limit of quantification values were 2.622 and 7.97. A sample throughput of 90 samples. hour1- . The new approach was effectively employed to determine the presence of hydrochlorothiazide in the pure, biological, and pharmaceutical samples.
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institution Kabale University
issn 2078-8665
2411-7986
language English
publishDate 2024-09-01
publisher University of Baghdad, College of Science for Women
record_format Article
series مجلة بغداد للعلوم
spelling doaj-art-bcedba0ea557459ab290c1ea023b9b932025-08-20T03:36:03ZengUniversity of Baghdad, College of Science for Womenمجلة بغداد للعلوم2078-86652411-79862024-09-0121910.21123/bsj.2024.8460Sensitive Determination of Hydrochlorothiazide Drug in the Various Samples Via Developed Method of CFIA System Wasan A. Hanooan0https://orcid.org/0009-0009-6982-8133Bushra B. Qassim1 Department of Chemistry, College of Science, University of Baghdad, Baghdad, Iraq. & Department of Pharmaceutical Chemistry, College of Pharmacy, University of Mustansiriyah, Baghdad, IraqDepartment of Pharmaceutical Chemistry, College of Pharmacy, University of Mustansiriyah, Baghdad, Iraq Hydrochlorothiazide drug determination in pharmaceutical and biological urine samples using a novel continuous flow-injection analysis technique that is rapid, simple, economical, and sensitive. The complex formed by the reaction of hydrochlorothiazide and O-phenylenediamine with potassium ferricyanide to produce an orange-colored product at 480 nm was the basis for the proposed method. Other chemical and physical parameters that affect the stability and development of the colored product used in this developed method includes sample volume, flow rate, reagent concentrations, and reaction coil. The proposed method’s linearity ranges from 5 to 150 μg.mL-1 and its correlation coefficients were 0.9988, and the recovery percentage and error percentage were 100.88 and 0.88, respectively. The detection limit and Limit of quantification values were 2.622 and 7.97. A sample throughput of 90 samples. hour1- . The new approach was effectively employed to determine the presence of hydrochlorothiazide in the pure, biological, and pharmaceutical samples. https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/8460Biological samples, CFIA System, Hydrochlorothiazide, O-phenylenediamine, Pharmaceutical formulation
spellingShingle Wasan A. Hanooan
Bushra B. Qassim
Sensitive Determination of Hydrochlorothiazide Drug in the Various Samples Via Developed Method of CFIA System
مجلة بغداد للعلوم
Biological samples, CFIA System, Hydrochlorothiazide, O-phenylenediamine, Pharmaceutical formulation
title Sensitive Determination of Hydrochlorothiazide Drug in the Various Samples Via Developed Method of CFIA System
title_full Sensitive Determination of Hydrochlorothiazide Drug in the Various Samples Via Developed Method of CFIA System
title_fullStr Sensitive Determination of Hydrochlorothiazide Drug in the Various Samples Via Developed Method of CFIA System
title_full_unstemmed Sensitive Determination of Hydrochlorothiazide Drug in the Various Samples Via Developed Method of CFIA System
title_short Sensitive Determination of Hydrochlorothiazide Drug in the Various Samples Via Developed Method of CFIA System
title_sort sensitive determination of hydrochlorothiazide drug in the various samples via developed method of cfia system
topic Biological samples, CFIA System, Hydrochlorothiazide, O-phenylenediamine, Pharmaceutical formulation
url https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/8460
work_keys_str_mv AT wasanahanooan sensitivedeterminationofhydrochlorothiazidedruginthevarioussamplesviadevelopedmethodofcfiasystem
AT bushrabqassim sensitivedeterminationofhydrochlorothiazidedruginthevarioussamplesviadevelopedmethodofcfiasystem