3D transcranial ultrasound localization microscopy in awake mice: protocol and open-source pipeline
Abstract We present an open-source code for 3D super-resolution ultrasound imaging. Open-3DULM was applied to transcranial imaging in habituated awake mice. Comparative analysis reveals that isoflurane anesthesia induces significant vasodilation and increased cerebral blood flow (CBF) in veins, whil...
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| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-06-01
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| Series: | Communications Engineering |
| Online Access: | https://doi.org/10.1038/s44172-025-00415-4 |
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| author | Georges Chabouh Louise Denis Myriam Abioui-Mourgues Jean-Baptiste Deloges Jacques Battaglia Arthur Chavignon Baptiste Heiles Basile Pradier Sylvain Bodard Denis Vivien Cyrille Orset Olivier Couture |
| author_facet | Georges Chabouh Louise Denis Myriam Abioui-Mourgues Jean-Baptiste Deloges Jacques Battaglia Arthur Chavignon Baptiste Heiles Basile Pradier Sylvain Bodard Denis Vivien Cyrille Orset Olivier Couture |
| author_sort | Georges Chabouh |
| collection | DOAJ |
| description | Abstract We present an open-source code for 3D super-resolution ultrasound imaging. Open-3DULM was applied to transcranial imaging in habituated awake mice. Comparative analysis reveals that isoflurane anesthesia induces significant vasodilation and increased cerebral blood flow (CBF) in veins, while only a subset of arteries exhibits these effects compared to the awake state. This method could serve as a reference for developing new types of 3D vascular quantifications, particularly emphasizing the importance of awake and 3D imaging for accurate cerebral blood flow measurement in neuroscience research. |
| format | Article |
| id | doaj-art-bced032a89e440a19fccc1176cf975e7 |
| institution | OA Journals |
| issn | 2731-3395 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Engineering |
| spelling | doaj-art-bced032a89e440a19fccc1176cf975e72025-08-20T02:05:38ZengNature PortfolioCommunications Engineering2731-33952025-06-01411810.1038/s44172-025-00415-43D transcranial ultrasound localization microscopy in awake mice: protocol and open-source pipelineGeorges Chabouh0Louise Denis1Myriam Abioui-Mourgues2Jean-Baptiste Deloges3Jacques Battaglia4Arthur Chavignon5Baptiste Heiles6Basile Pradier7Sylvain Bodard8Denis Vivien9Cyrille Orset10Olivier Couture11Sorbonne Université, UMR 7371 CNRS, Inserm U1146, Laboratoire d’Imagerie BiomedicaleSorbonne Université, UMR 7371 CNRS, Inserm U1146, Laboratoire d’Imagerie BiomedicaleUNICAEN, Caen Normandie Université,Inserm UMR-S U1237, Physiopathology and Imaging of Neurological Disorders (PhIND), GIP Cyceron, Institut Blood and Brain @ Caen-Normandie (BB@C), Normandie UniversitySorbonne Université, UMR 7371 CNRS, Inserm U1146, Laboratoire d’Imagerie BiomedicaleSorbonne Université, UMR 7371 CNRS, Inserm U1146, Laboratoire d’Imagerie BiomedicaleSorbonne Université, UMR 7371 CNRS, Inserm U1146, Laboratoire d’Imagerie BiomedicaleDivision of Chemistry and Chemical Engineering, California Institute of TechnologySorbonne Université, UMR 7371 CNRS, Inserm U1146, Laboratoire d’Imagerie BiomedicaleSorbonne Université, UMR 7371 CNRS, Inserm U1146, Laboratoire d’Imagerie BiomedicaleUNICAEN, Caen Normandie Université,Inserm UMR-S U1237, Physiopathology and Imaging of Neurological Disorders (PhIND), GIP Cyceron, Institut Blood and Brain @ Caen-Normandie (BB@C), Normandie UniversityUNICAEN, Caen Normandie Université,Inserm UMR-S U1237, Physiopathology and Imaging of Neurological Disorders (PhIND), GIP Cyceron, Institut Blood and Brain @ Caen-Normandie (BB@C), Normandie UniversitySorbonne Université, UMR 7371 CNRS, Inserm U1146, Laboratoire d’Imagerie BiomedicaleAbstract We present an open-source code for 3D super-resolution ultrasound imaging. Open-3DULM was applied to transcranial imaging in habituated awake mice. Comparative analysis reveals that isoflurane anesthesia induces significant vasodilation and increased cerebral blood flow (CBF) in veins, while only a subset of arteries exhibits these effects compared to the awake state. This method could serve as a reference for developing new types of 3D vascular quantifications, particularly emphasizing the importance of awake and 3D imaging for accurate cerebral blood flow measurement in neuroscience research.https://doi.org/10.1038/s44172-025-00415-4 |
| spellingShingle | Georges Chabouh Louise Denis Myriam Abioui-Mourgues Jean-Baptiste Deloges Jacques Battaglia Arthur Chavignon Baptiste Heiles Basile Pradier Sylvain Bodard Denis Vivien Cyrille Orset Olivier Couture 3D transcranial ultrasound localization microscopy in awake mice: protocol and open-source pipeline Communications Engineering |
| title | 3D transcranial ultrasound localization microscopy in awake mice: protocol and open-source pipeline |
| title_full | 3D transcranial ultrasound localization microscopy in awake mice: protocol and open-source pipeline |
| title_fullStr | 3D transcranial ultrasound localization microscopy in awake mice: protocol and open-source pipeline |
| title_full_unstemmed | 3D transcranial ultrasound localization microscopy in awake mice: protocol and open-source pipeline |
| title_short | 3D transcranial ultrasound localization microscopy in awake mice: protocol and open-source pipeline |
| title_sort | 3d transcranial ultrasound localization microscopy in awake mice protocol and open source pipeline |
| url | https://doi.org/10.1038/s44172-025-00415-4 |
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