The Association Between Basidiomycete Fungi and Mealybugs Pseudococcidae Affects Coffee Plants
Some Pseudococcidae species interact with <i>Coffea arabica</i>’s roots and are associated with basidiomycete fungi. The fungal mycelium envelops the roots, which hinders their water and nutrient absorption. Combined with the feeding activity of the insects, this results in chlorosis, de...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-02-01
|
| Series: | Agronomy |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4395/15/3/551 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849392726072623104 |
|---|---|
| author | Luz Andrea Carmona-Valencia Lucio Navarro-Escalante Pablo Benavides Zulma Nancy Gil Palacio |
| author_facet | Luz Andrea Carmona-Valencia Lucio Navarro-Escalante Pablo Benavides Zulma Nancy Gil Palacio |
| author_sort | Luz Andrea Carmona-Valencia |
| collection | DOAJ |
| description | Some Pseudococcidae species interact with <i>Coffea arabica</i>’s roots and are associated with basidiomycete fungi. The fungal mycelium envelops the roots, which hinders their water and nutrient absorption. Combined with the feeding activity of the insects, this results in chlorosis, defoliation, and even plant death. Despite the significance of these interactions, they remain under-studied. To investigate the relationship between sporocarps found at the base of coffee trees, the cysts covering their roots, and the mealybug insects within them, samples of these three organisms—sporocarps, cysts, and mealybugs—were collected from 27 coffee plants across three farms in the departments of Norte de Santander and Quindío, Colombia. Fungi and cysts were identified by sequencing a nuclear gene region of the 28S large ribosomal subunit (28S rDNA) using the primers LSU200-F and LSU481-R. Fungal identification was further confirmed through classical taxonomy. Mealybugs were identified by sequencing a region of the mitochondrial gene Cytochrome C oxidase subunit I (COI) with CIF-CIR primers, corroborated through classical taxonomy. This study identified four fungal species associated with four species of <i>Pseudococcidae</i>. The fungus <i>Phlebopus beniensis</i> was associated with the mealybugs <i>Pseudococcus elisae</i>, <i>Dysmicoccus neobrevipes</i>, <i>D. brevipes</i>, and <i>Pseudococcus nr. sociabilis</i>. <i>Phlebopus portentosus</i> was linked to <i>D. neobrevipes</i>, while <i>Xerophorus olivascens</i> and <i>Boletinellus rompelii</i> were associated with other <i>Pseudococcidae</i> species. Additionally, the fungus <i>Pseudolaccaria pachyphylla</i> was found in coffee plants harboring mealybugs. These findings confirm the existence of specific associations between fungal species and mealybug insects that affect coffee plants. |
| format | Article |
| id | doaj-art-bce04b1839994626bb8aaaed04286a67 |
| institution | Kabale University |
| issn | 2073-4395 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj-art-bce04b1839994626bb8aaaed04286a672025-08-20T03:40:42ZengMDPI AGAgronomy2073-43952025-02-0115355110.3390/agronomy15030551The Association Between Basidiomycete Fungi and Mealybugs Pseudococcidae Affects Coffee PlantsLuz Andrea Carmona-Valencia0Lucio Navarro-Escalante1Pablo Benavides2Zulma Nancy Gil Palacio3Department of Entomology, National Coffee Research Center, Cenicafé, Manizales 170009, ColombiaDepartment of Molecular Biosciences, University of Texas at Austin, Austin, TX 78712, USADepartment of Entomology, National Coffee Research Center, Cenicafé, Manizales 170009, ColombiaDepartment of Entomology, National Coffee Research Center, Cenicafé, Manizales 170009, ColombiaSome Pseudococcidae species interact with <i>Coffea arabica</i>’s roots and are associated with basidiomycete fungi. The fungal mycelium envelops the roots, which hinders their water and nutrient absorption. Combined with the feeding activity of the insects, this results in chlorosis, defoliation, and even plant death. Despite the significance of these interactions, they remain under-studied. To investigate the relationship between sporocarps found at the base of coffee trees, the cysts covering their roots, and the mealybug insects within them, samples of these three organisms—sporocarps, cysts, and mealybugs—were collected from 27 coffee plants across three farms in the departments of Norte de Santander and Quindío, Colombia. Fungi and cysts were identified by sequencing a nuclear gene region of the 28S large ribosomal subunit (28S rDNA) using the primers LSU200-F and LSU481-R. Fungal identification was further confirmed through classical taxonomy. Mealybugs were identified by sequencing a region of the mitochondrial gene Cytochrome C oxidase subunit I (COI) with CIF-CIR primers, corroborated through classical taxonomy. This study identified four fungal species associated with four species of <i>Pseudococcidae</i>. The fungus <i>Phlebopus beniensis</i> was associated with the mealybugs <i>Pseudococcus elisae</i>, <i>Dysmicoccus neobrevipes</i>, <i>D. brevipes</i>, and <i>Pseudococcus nr. sociabilis</i>. <i>Phlebopus portentosus</i> was linked to <i>D. neobrevipes</i>, while <i>Xerophorus olivascens</i> and <i>Boletinellus rompelii</i> were associated with other <i>Pseudococcidae</i> species. Additionally, the fungus <i>Pseudolaccaria pachyphylla</i> was found in coffee plants harboring mealybugs. These findings confirm the existence of specific associations between fungal species and mealybug insects that affect coffee plants.https://www.mdpi.com/2073-4395/15/3/551biotrophiccoffeemealybugsmutualismrootssporocarps |
| spellingShingle | Luz Andrea Carmona-Valencia Lucio Navarro-Escalante Pablo Benavides Zulma Nancy Gil Palacio The Association Between Basidiomycete Fungi and Mealybugs Pseudococcidae Affects Coffee Plants Agronomy biotrophic coffee mealybugs mutualism roots sporocarps |
| title | The Association Between Basidiomycete Fungi and Mealybugs Pseudococcidae Affects Coffee Plants |
| title_full | The Association Between Basidiomycete Fungi and Mealybugs Pseudococcidae Affects Coffee Plants |
| title_fullStr | The Association Between Basidiomycete Fungi and Mealybugs Pseudococcidae Affects Coffee Plants |
| title_full_unstemmed | The Association Between Basidiomycete Fungi and Mealybugs Pseudococcidae Affects Coffee Plants |
| title_short | The Association Between Basidiomycete Fungi and Mealybugs Pseudococcidae Affects Coffee Plants |
| title_sort | association between basidiomycete fungi and mealybugs pseudococcidae affects coffee plants |
| topic | biotrophic coffee mealybugs mutualism roots sporocarps |
| url | https://www.mdpi.com/2073-4395/15/3/551 |
| work_keys_str_mv | AT luzandreacarmonavalencia theassociationbetweenbasidiomycetefungiandmealybugspseudococcidaeaffectscoffeeplants AT lucionavarroescalante theassociationbetweenbasidiomycetefungiandmealybugspseudococcidaeaffectscoffeeplants AT pablobenavides theassociationbetweenbasidiomycetefungiandmealybugspseudococcidaeaffectscoffeeplants AT zulmanancygilpalacio theassociationbetweenbasidiomycetefungiandmealybugspseudococcidaeaffectscoffeeplants AT luzandreacarmonavalencia associationbetweenbasidiomycetefungiandmealybugspseudococcidaeaffectscoffeeplants AT lucionavarroescalante associationbetweenbasidiomycetefungiandmealybugspseudococcidaeaffectscoffeeplants AT pablobenavides associationbetweenbasidiomycetefungiandmealybugspseudococcidaeaffectscoffeeplants AT zulmanancygilpalacio associationbetweenbasidiomycetefungiandmealybugspseudococcidaeaffectscoffeeplants |