Metagenomic insight into the vaginal microbiome in women infected with HPV 16 and 18
Abstract Human papillomavirus (HPV) 16 and 18 (HPV 16/18) account for over 70% of cervical cancer (CC) cases, yet their interaction with the vaginal microbiome (VM) remains unclear. This study explored the association between high-risk HPV types (HR-HPVs), VM composition and bacterial function using...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-06-01
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| Series: | npj Biofilms and Microbiomes |
| Online Access: | https://doi.org/10.1038/s41522-025-00747-1 |
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| author | Da-Ryung Jung Yeseul Choi Minsoo Jeong Vineet Singh Se Young Jeon Incheol Seo Nora Jee-Young Park Yoon Hee Lee Ji Young Park Hyung Soo Han Jae-Ho Shin Gun Oh Chong |
| author_facet | Da-Ryung Jung Yeseul Choi Minsoo Jeong Vineet Singh Se Young Jeon Incheol Seo Nora Jee-Young Park Yoon Hee Lee Ji Young Park Hyung Soo Han Jae-Ho Shin Gun Oh Chong |
| author_sort | Da-Ryung Jung |
| collection | DOAJ |
| description | Abstract Human papillomavirus (HPV) 16 and 18 (HPV 16/18) account for over 70% of cervical cancer (CC) cases, yet their interaction with the vaginal microbiome (VM) remains unclear. This study explored the association between high-risk HPV types (HR-HPVs), VM composition and bacterial function using shotgun metagenomic sequencing. In early-stage cervical lesions, the HPV 16/18 group showed reduced Lactobacillus-dominant community state types compared to other HR-HPVs, while invasive CC exhibited increased pathogenic bacteria, including Streptococcus agalactiae, Fannyhessea vaginae, and Sneathia vaginalis. The VM associated with HPV 16/18 was enriched in immune response and inflammation pathways, whereas other HR-HPVs were linked to cellular metabolism and hormonal signaling. Notably, HPV 16/18 exhibited stronger bacterial-fungal correlations, indicating shifts in the microbial community. Furthermore, 137 metagenome-assembled genomes provided insights into unique microbial genomic signatures. Our study links VM differences with HPV 16/18 oncogenic potential across cervical lesion stages, urging further research for better diagnostics and treatments. |
| format | Article |
| id | doaj-art-bcd3d5fa58e146dfb67870c6aec06ebc |
| institution | OA Journals |
| issn | 2055-5008 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Biofilms and Microbiomes |
| spelling | doaj-art-bcd3d5fa58e146dfb67870c6aec06ebc2025-08-20T02:06:20ZengNature Portfolionpj Biofilms and Microbiomes2055-50082025-06-0111111310.1038/s41522-025-00747-1Metagenomic insight into the vaginal microbiome in women infected with HPV 16 and 18Da-Ryung Jung0Yeseul Choi1Minsoo Jeong2Vineet Singh3Se Young Jeon4Incheol Seo5Nora Jee-Young Park6Yoon Hee Lee7Ji Young Park8Hyung Soo Han9Jae-Ho Shin10Gun Oh Chong11Department of Applied Biosciences, Kyungpook National UniversityDepartment of Biomedical Science, Graduate School, Kyungpook National UniversityDepartment of Applied Biosciences, Kyungpook National UniversityDepartment of Applied Biosciences, Kyungpook National UniversityDepartment of Obstetrics and Gynecology, Kyungpook National University Chilgok HospitalClinical Omics Institute, Kyungpook National UniversityClinical Omics Institute, Kyungpook National UniversityDepartment of Obstetrics and Gynecology, Kyungpook National University Chilgok HospitalDepartment of Pathology, Kyungpook National University Chilgok HospitalDepartment of Biomedical Science, Graduate School, Kyungpook National UniversityDepartment of Applied Biosciences, Kyungpook National UniversityDepartment of Obstetrics and Gynecology, Kyungpook National University Chilgok HospitalAbstract Human papillomavirus (HPV) 16 and 18 (HPV 16/18) account for over 70% of cervical cancer (CC) cases, yet their interaction with the vaginal microbiome (VM) remains unclear. This study explored the association between high-risk HPV types (HR-HPVs), VM composition and bacterial function using shotgun metagenomic sequencing. In early-stage cervical lesions, the HPV 16/18 group showed reduced Lactobacillus-dominant community state types compared to other HR-HPVs, while invasive CC exhibited increased pathogenic bacteria, including Streptococcus agalactiae, Fannyhessea vaginae, and Sneathia vaginalis. The VM associated with HPV 16/18 was enriched in immune response and inflammation pathways, whereas other HR-HPVs were linked to cellular metabolism and hormonal signaling. Notably, HPV 16/18 exhibited stronger bacterial-fungal correlations, indicating shifts in the microbial community. Furthermore, 137 metagenome-assembled genomes provided insights into unique microbial genomic signatures. Our study links VM differences with HPV 16/18 oncogenic potential across cervical lesion stages, urging further research for better diagnostics and treatments.https://doi.org/10.1038/s41522-025-00747-1 |
| spellingShingle | Da-Ryung Jung Yeseul Choi Minsoo Jeong Vineet Singh Se Young Jeon Incheol Seo Nora Jee-Young Park Yoon Hee Lee Ji Young Park Hyung Soo Han Jae-Ho Shin Gun Oh Chong Metagenomic insight into the vaginal microbiome in women infected with HPV 16 and 18 npj Biofilms and Microbiomes |
| title | Metagenomic insight into the vaginal microbiome in women infected with HPV 16 and 18 |
| title_full | Metagenomic insight into the vaginal microbiome in women infected with HPV 16 and 18 |
| title_fullStr | Metagenomic insight into the vaginal microbiome in women infected with HPV 16 and 18 |
| title_full_unstemmed | Metagenomic insight into the vaginal microbiome in women infected with HPV 16 and 18 |
| title_short | Metagenomic insight into the vaginal microbiome in women infected with HPV 16 and 18 |
| title_sort | metagenomic insight into the vaginal microbiome in women infected with hpv 16 and 18 |
| url | https://doi.org/10.1038/s41522-025-00747-1 |
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