Domain characteristics, classification and expression profiles in response to various abiotic stresses of four HD-Zip subfamilies in tea plant

Abstract Background The homeodomain-leucine zipper (HD-Zip) transcription factors play crucial roles in plant growth and development or in response to various abiotic stresses. Results In our research, fifty-eight HD-Zip genes were identified in tea plant, namely CsHDZ01-58 and divided into four sub...

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Main Authors: Xiaowen Luo, Jiao Lu, Yunye Li, Xinfu Zhang, Liping Gao, Tao Xia, Yahui Han
Format: Article
Language:English
Published: BMC 2025-06-01
Series:BMC Plant Biology
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Online Access:https://doi.org/10.1186/s12870-025-06619-2
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author Xiaowen Luo
Jiao Lu
Yunye Li
Xinfu Zhang
Liping Gao
Tao Xia
Yahui Han
author_facet Xiaowen Luo
Jiao Lu
Yunye Li
Xinfu Zhang
Liping Gao
Tao Xia
Yahui Han
author_sort Xiaowen Luo
collection DOAJ
description Abstract Background The homeodomain-leucine zipper (HD-Zip) transcription factors play crucial roles in plant growth and development or in response to various abiotic stresses. Results In our research, fifty-eight HD-Zip genes were identified in tea plant, namely CsHDZ01-58 and divided into four subfamilies, I-IV. All the CsHDZ proteins of four subfamilies harbored HD and LZ domains. Subfamily II CsHDZs contained two additional motifs, EAR and CPSCE. Two extra characteristic domains, START and SAD, were included in subfamily III and IV CsHDZs. While MEKHLA domain was distinctive in subfamily III members. All the subfamily III CsHDZs were predicted to be targets of csn-miR166. A total of 24 duplicated CsHDZ gene pairs were identified and generated by segmental duplication in tea plant. The expression analysis indicated that most subfamily IV CsHDZ genes were expressed at high level in apical bud, and most subfamily III CsHDZs were high-expressed in stem. CsHDZ family genes exibited diverse expression profiles under cold, drought and salt stresses respectively. It implied that CsHDZ gene family may participate in regulating the response to various abiotic stresses in tea plant. Conclusion These results will provide an important foundation for further exploring the function and molecular mechanism of HD-Zip genes in response to various abiotic stresses in tea plant.
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institution Kabale University
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publishDate 2025-06-01
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series BMC Plant Biology
spelling doaj-art-bcceb3523d5f405ab86cbd6d93ebae562025-08-20T03:26:42ZengBMCBMC Plant Biology1471-22292025-06-0125112210.1186/s12870-025-06619-2Domain characteristics, classification and expression profiles in response to various abiotic stresses of four HD-Zip subfamilies in tea plantXiaowen Luo0Jiao Lu1Yunye Li2Xinfu Zhang3Liping Gao4Tao Xia5Yahui Han6College of Horticulture, Qingdao Agricultural UniversityCollege of Horticulture, Qingdao Agricultural UniversityCollege of Horticulture, Qingdao Agricultural UniversityCollege of Horticulture, Qingdao Agricultural UniversitySchool of Life Science, Anhui Agricultural UniversityState Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural UniversityCollege of Horticulture, Qingdao Agricultural UniversityAbstract Background The homeodomain-leucine zipper (HD-Zip) transcription factors play crucial roles in plant growth and development or in response to various abiotic stresses. Results In our research, fifty-eight HD-Zip genes were identified in tea plant, namely CsHDZ01-58 and divided into four subfamilies, I-IV. All the CsHDZ proteins of four subfamilies harbored HD and LZ domains. Subfamily II CsHDZs contained two additional motifs, EAR and CPSCE. Two extra characteristic domains, START and SAD, were included in subfamily III and IV CsHDZs. While MEKHLA domain was distinctive in subfamily III members. All the subfamily III CsHDZs were predicted to be targets of csn-miR166. A total of 24 duplicated CsHDZ gene pairs were identified and generated by segmental duplication in tea plant. The expression analysis indicated that most subfamily IV CsHDZ genes were expressed at high level in apical bud, and most subfamily III CsHDZs were high-expressed in stem. CsHDZ family genes exibited diverse expression profiles under cold, drought and salt stresses respectively. It implied that CsHDZ gene family may participate in regulating the response to various abiotic stresses in tea plant. Conclusion These results will provide an important foundation for further exploring the function and molecular mechanism of HD-Zip genes in response to various abiotic stresses in tea plant.https://doi.org/10.1186/s12870-025-06619-2HD-ZipTea plantDomain characteristicExpression patternAbiotic stress
spellingShingle Xiaowen Luo
Jiao Lu
Yunye Li
Xinfu Zhang
Liping Gao
Tao Xia
Yahui Han
Domain characteristics, classification and expression profiles in response to various abiotic stresses of four HD-Zip subfamilies in tea plant
BMC Plant Biology
HD-Zip
Tea plant
Domain characteristic
Expression pattern
Abiotic stress
title Domain characteristics, classification and expression profiles in response to various abiotic stresses of four HD-Zip subfamilies in tea plant
title_full Domain characteristics, classification and expression profiles in response to various abiotic stresses of four HD-Zip subfamilies in tea plant
title_fullStr Domain characteristics, classification and expression profiles in response to various abiotic stresses of four HD-Zip subfamilies in tea plant
title_full_unstemmed Domain characteristics, classification and expression profiles in response to various abiotic stresses of four HD-Zip subfamilies in tea plant
title_short Domain characteristics, classification and expression profiles in response to various abiotic stresses of four HD-Zip subfamilies in tea plant
title_sort domain characteristics classification and expression profiles in response to various abiotic stresses of four hd zip subfamilies in tea plant
topic HD-Zip
Tea plant
Domain characteristic
Expression pattern
Abiotic stress
url https://doi.org/10.1186/s12870-025-06619-2
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