Domain characteristics, classification and expression profiles in response to various abiotic stresses of four HD-Zip subfamilies in tea plant

Abstract Background The homeodomain-leucine zipper (HD-Zip) transcription factors play crucial roles in plant growth and development or in response to various abiotic stresses. Results In our research, fifty-eight HD-Zip genes were identified in tea plant, namely CsHDZ01-58 and divided into four sub...

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Main Authors: Xiaowen Luo, Jiao Lu, Yunye Li, Xinfu Zhang, Liping Gao, Tao Xia, Yahui Han
Format: Article
Language:English
Published: BMC 2025-06-01
Series:BMC Plant Biology
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Online Access:https://doi.org/10.1186/s12870-025-06619-2
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Summary:Abstract Background The homeodomain-leucine zipper (HD-Zip) transcription factors play crucial roles in plant growth and development or in response to various abiotic stresses. Results In our research, fifty-eight HD-Zip genes were identified in tea plant, namely CsHDZ01-58 and divided into four subfamilies, I-IV. All the CsHDZ proteins of four subfamilies harbored HD and LZ domains. Subfamily II CsHDZs contained two additional motifs, EAR and CPSCE. Two extra characteristic domains, START and SAD, were included in subfamily III and IV CsHDZs. While MEKHLA domain was distinctive in subfamily III members. All the subfamily III CsHDZs were predicted to be targets of csn-miR166. A total of 24 duplicated CsHDZ gene pairs were identified and generated by segmental duplication in tea plant. The expression analysis indicated that most subfamily IV CsHDZ genes were expressed at high level in apical bud, and most subfamily III CsHDZs were high-expressed in stem. CsHDZ family genes exibited diverse expression profiles under cold, drought and salt stresses respectively. It implied that CsHDZ gene family may participate in regulating the response to various abiotic stresses in tea plant. Conclusion These results will provide an important foundation for further exploring the function and molecular mechanism of HD-Zip genes in response to various abiotic stresses in tea plant.
ISSN:1471-2229