Dynamic basis of supercoiling-dependent DNA interrogation by Cas12a via R-loop intermediates

Dynamic basis of supercoiling-dependent DNA interrogation by Cas12a via R-loop intermediates

Abstract The sequence specificity and programmability of DNA binding and cleavage have enabled widespread applications of CRISPR-Cas12a in genetic engineering. As an RNA-guided CRISPR endonuclease, Cas12a engages a 20-base pair (bp) DNA segment by forming a three-stranded R-loop structure in which t...

Full description

Saved in:
Bibliographic Details
Main Authors: Kevin D. P. Aris, Joshua C. Cofsky, Honglue Shi, Noor Al-Sayyad, Ivan E. Ivanov, Ashwin Balaji, Jennifer A. Doudna, Zev Bryant
Format: Article
Language:English
Published: Nature Portfolio 2025-03-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-025-57703-y
Tags: Add Tag
No Tags, Be the first to tag this record!

Similar Items

Similar Items