Quantitative photoacoustic imaging using known chromophores as fluence marker
Photoacoustic imaging offers optical contrast images of human tissue at acoustic resolution, making it valuable for diverse clinical applications. However, quantifying tissue composition via optical contrast remains challenging due to the unknown light fluence within the tissue. Here, we propose a m...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-02-01
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| Series: | Photoacoustics |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213597924000909 |
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| author | Anjali Thomas Max Rietberg Mervenur Akkus Gijs van Soest Kalloor Joseph Francis |
| author_facet | Anjali Thomas Max Rietberg Mervenur Akkus Gijs van Soest Kalloor Joseph Francis |
| author_sort | Anjali Thomas |
| collection | DOAJ |
| description | Photoacoustic imaging offers optical contrast images of human tissue at acoustic resolution, making it valuable for diverse clinical applications. However, quantifying tissue composition via optical contrast remains challenging due to the unknown light fluence within the tissue. Here, we propose a method that leverages known chromophores (e.g., arterial blood) to improve the accuracy of quantitative photoacoustic imaging. By using the optical properties of a known chromophore as a fluence marker and integrating it into the optical inversion process, we can estimate the unknown fluence within the tissue. Experimentally, we demonstrate that this approach successfully recovers both the spectral shape and magnitude of the optical absorption coefficient of an unknown chromophore. Additionally, we show that the fluence marker method enhances conventional optical inversion techniques, specifically (i) a straightforward iterative approach and (ii) a gradient-based method. Our results indicate an improvement in accuracy of up to 24.4% when comparing optical absorption recovery with and without the fluence marker. Finally, we present the method’s performance and illustrate its applications in carotid plaque quantification. |
| format | Article |
| id | doaj-art-bc7e7696d03f4195b34035a3be6dd5d3 |
| institution | Kabale University |
| issn | 2213-5979 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Photoacoustics |
| spelling | doaj-art-bc7e7696d03f4195b34035a3be6dd5d32025-01-17T04:49:30ZengElsevierPhotoacoustics2213-59792025-02-0141100673Quantitative photoacoustic imaging using known chromophores as fluence markerAnjali Thomas0Max Rietberg1Mervenur Akkus2Gijs van Soest3Kalloor Joseph Francis4Biomedical Photonic Imaging Group, Technical Medical Center, University of Twente, Enschede, 7522 NB, The Netherlands; Erasmus MC, Cardiovascular Institute, Department of Cardiology, Biomedical Engineering, Rotterdam, The NetherlandsBiomedical Photonic Imaging Group, Technical Medical Center, University of Twente, Enschede, 7522 NB, The NetherlandsErasmus MC, Cardiovascular Institute, Department of Cardiology, Biomedical Engineering, Rotterdam, The NetherlandsErasmus MC, Cardiovascular Institute, Department of Cardiology, Biomedical Engineering, Rotterdam, The Netherlands; Department of Precision and Microsystems Engineering, Delft University of Technology, Delft, The Netherlands; Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, USAErasmus MC, Cardiovascular Institute, Department of Cardiology, Biomedical Engineering, Rotterdam, The Netherlands; Corresponding author.Photoacoustic imaging offers optical contrast images of human tissue at acoustic resolution, making it valuable for diverse clinical applications. However, quantifying tissue composition via optical contrast remains challenging due to the unknown light fluence within the tissue. Here, we propose a method that leverages known chromophores (e.g., arterial blood) to improve the accuracy of quantitative photoacoustic imaging. By using the optical properties of a known chromophore as a fluence marker and integrating it into the optical inversion process, we can estimate the unknown fluence within the tissue. Experimentally, we demonstrate that this approach successfully recovers both the spectral shape and magnitude of the optical absorption coefficient of an unknown chromophore. Additionally, we show that the fluence marker method enhances conventional optical inversion techniques, specifically (i) a straightforward iterative approach and (ii) a gradient-based method. Our results indicate an improvement in accuracy of up to 24.4% when comparing optical absorption recovery with and without the fluence marker. Finally, we present the method’s performance and illustrate its applications in carotid plaque quantification.http://www.sciencedirect.com/science/article/pii/S2213597924000909Photoacoustic imagingTissue quantificationOptical inversionFluence correctionFluence markerSpectral imaging |
| spellingShingle | Anjali Thomas Max Rietberg Mervenur Akkus Gijs van Soest Kalloor Joseph Francis Quantitative photoacoustic imaging using known chromophores as fluence marker Photoacoustics Photoacoustic imaging Tissue quantification Optical inversion Fluence correction Fluence marker Spectral imaging |
| title | Quantitative photoacoustic imaging using known chromophores as fluence marker |
| title_full | Quantitative photoacoustic imaging using known chromophores as fluence marker |
| title_fullStr | Quantitative photoacoustic imaging using known chromophores as fluence marker |
| title_full_unstemmed | Quantitative photoacoustic imaging using known chromophores as fluence marker |
| title_short | Quantitative photoacoustic imaging using known chromophores as fluence marker |
| title_sort | quantitative photoacoustic imaging using known chromophores as fluence marker |
| topic | Photoacoustic imaging Tissue quantification Optical inversion Fluence correction Fluence marker Spectral imaging |
| url | http://www.sciencedirect.com/science/article/pii/S2213597924000909 |
| work_keys_str_mv | AT anjalithomas quantitativephotoacousticimagingusingknownchromophoresasfluencemarker AT maxrietberg quantitativephotoacousticimagingusingknownchromophoresasfluencemarker AT mervenurakkus quantitativephotoacousticimagingusingknownchromophoresasfluencemarker AT gijsvansoest quantitativephotoacousticimagingusingknownchromophoresasfluencemarker AT kalloorjosephfrancis quantitativephotoacousticimagingusingknownchromophoresasfluencemarker |