PiERF1 regulates cold tolerance in Plumbago indica L. through ethylene signalling
Abstract Ethylene is a signalling factor that plays a key role in the response of plants to abiotic stresses, such as cold stress. Recent studies have shown that the exogenous application of 1-aminocyclopropane-1-carboxylate (ACC), an ethylene promoter, affects plant cold tolerance. The cold-respons...
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Nature Portfolio
2025-01-01
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| Online Access: | https://doi.org/10.1038/s41598-025-86057-0 |
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| author | Zi-An Zhao Yi-Rui Li Ting Lei Cai-Lei Liu Qing-Xiao Zeng Xuan Liu Li-Juan Yang Jia-Ni Li Su-Ping Gao |
| author_facet | Zi-An Zhao Yi-Rui Li Ting Lei Cai-Lei Liu Qing-Xiao Zeng Xuan Liu Li-Juan Yang Jia-Ni Li Su-Ping Gao |
| author_sort | Zi-An Zhao |
| collection | DOAJ |
| description | Abstract Ethylene is a signalling factor that plays a key role in the response of plants to abiotic stresses, such as cold stress. Recent studies have shown that the exogenous application of 1-aminocyclopropane-1-carboxylate (ACC), an ethylene promoter, affects plant cold tolerance. The cold-responsive specific gene DREB plays a crucial role in enhancing cold tolerance in plants by activating several cold-responsive (COR) genes. However, how the ethylene biosynthesis pathway regulates this gene in the cold response of thermophilic plants has yet to be fully elucidated. In this study, the thermophilic plant Plumbago indica L. was used as an example. Physiological experiments and transcriptomic analyses revealed that cold stress treatment induced the synthesis of endogenous ACC and regulated the ethylene signalling activator PiERF1, and cold signalling also activated PiDREB1A. Spray experiments also revealed that ACC-induced upregulation of the PiERF1 gene reduced the cold tolerance of P. indica and decreased the expression level of the PiDREB1A gene. These results indicate that ethylene signalling directly regulates the downstream gene PiERF1 and initiates the DREB‒COR cold-responsive signalling pathway to regulate cold tolerance, resulting in the negative regulation of cold tolerance in thermophilic plants. |
| format | Article |
| id | doaj-art-bc7c8deef53a494db0c692cb500dbd9c |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-bc7c8deef53a494db0c692cb500dbd9c2025-01-12T12:22:14ZengNature PortfolioScientific Reports2045-23222025-01-0115111610.1038/s41598-025-86057-0PiERF1 regulates cold tolerance in Plumbago indica L. through ethylene signallingZi-An Zhao0Yi-Rui Li1Ting Lei2Cai-Lei Liu3Qing-Xiao Zeng4Xuan Liu5Li-Juan Yang6Jia-Ni Li7Su-Ping Gao8College of Landscape Architecture, Sichuan Agricultural UniversityCollege of Landscape Architecture, Sichuan Agricultural UniversityCollege of Landscape Architecture, Sichuan Agricultural UniversityCollege of Landscape Architecture, Sichuan Agricultural UniversityCollege of Landscape Architecture, Sichuan Agricultural UniversityCollege of Landscape Architecture, Sichuan Agricultural UniversityCollege of Landscape Architecture, Sichuan Agricultural UniversityCollege of Landscape Architecture, Sichuan Agricultural UniversityCollege of Landscape Architecture, Sichuan Agricultural UniversityAbstract Ethylene is a signalling factor that plays a key role in the response of plants to abiotic stresses, such as cold stress. Recent studies have shown that the exogenous application of 1-aminocyclopropane-1-carboxylate (ACC), an ethylene promoter, affects plant cold tolerance. The cold-responsive specific gene DREB plays a crucial role in enhancing cold tolerance in plants by activating several cold-responsive (COR) genes. However, how the ethylene biosynthesis pathway regulates this gene in the cold response of thermophilic plants has yet to be fully elucidated. In this study, the thermophilic plant Plumbago indica L. was used as an example. Physiological experiments and transcriptomic analyses revealed that cold stress treatment induced the synthesis of endogenous ACC and regulated the ethylene signalling activator PiERF1, and cold signalling also activated PiDREB1A. Spray experiments also revealed that ACC-induced upregulation of the PiERF1 gene reduced the cold tolerance of P. indica and decreased the expression level of the PiDREB1A gene. These results indicate that ethylene signalling directly regulates the downstream gene PiERF1 and initiates the DREB‒COR cold-responsive signalling pathway to regulate cold tolerance, resulting in the negative regulation of cold tolerance in thermophilic plants.https://doi.org/10.1038/s41598-025-86057-0Ethylene signalling pathwayPiERF1PiDREB1ACold tolerance1-aminocyclopropane-1-carboxylatePlumbago indica L. |
| spellingShingle | Zi-An Zhao Yi-Rui Li Ting Lei Cai-Lei Liu Qing-Xiao Zeng Xuan Liu Li-Juan Yang Jia-Ni Li Su-Ping Gao PiERF1 regulates cold tolerance in Plumbago indica L. through ethylene signalling Scientific Reports Ethylene signalling pathway PiERF1 PiDREB1A Cold tolerance 1-aminocyclopropane-1-carboxylate Plumbago indica L. |
| title | PiERF1 regulates cold tolerance in Plumbago indica L. through ethylene signalling |
| title_full | PiERF1 regulates cold tolerance in Plumbago indica L. through ethylene signalling |
| title_fullStr | PiERF1 regulates cold tolerance in Plumbago indica L. through ethylene signalling |
| title_full_unstemmed | PiERF1 regulates cold tolerance in Plumbago indica L. through ethylene signalling |
| title_short | PiERF1 regulates cold tolerance in Plumbago indica L. through ethylene signalling |
| title_sort | pierf1 regulates cold tolerance in plumbago indica l through ethylene signalling |
| topic | Ethylene signalling pathway PiERF1 PiDREB1A Cold tolerance 1-aminocyclopropane-1-carboxylate Plumbago indica L. |
| url | https://doi.org/10.1038/s41598-025-86057-0 |
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