Effects of Collagenase Preconditioning on Partially Incised Rat Tendon Treated with Light-Emitting Diodes and Platelet-Rich Plasma
<b>Background:</b> Tendinopathy is a challenging condition associated with high treatment costs, prolonged dysfunction, and lower quality of life. Current treatment strategies aim to accelerate healing by modulating the healing phases. Phototherapy and growth factor-based modalities have...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-05-01
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| Series: | Biomedicines |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2227-9059/13/5/1214 |
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| Summary: | <b>Background:</b> Tendinopathy is a challenging condition associated with high treatment costs, prolonged dysfunction, and lower quality of life. Current treatment strategies aim to accelerate healing by modulating the healing phases. Phototherapy and growth factor-based modalities have shown promising outcomes in promoting tendon healing. A two-factor experimental design investigates the therapeutic efficacy of conditioning a partially tenotomized rat Achilles tendon model with low concentrations of collagenase, followed by platelet-rich plasma and/or light-emitting diode treatments. <b>Methods:</b> Forty-six adult male Wistar rats (284.8g ± 6.8) were randomly assigned to nine groups (G1 (n = 6), G2–G9; n = 5 per group) based on the treatment applied upon a partially incised rat’s hind-limb Achilles tendon model for three weeks. On day 21, blood samples were collected for hematological and biochemical analyses and tendon explants were harvested and subjected to histology. <b>Results:</b> Observational findings support the safety and validity of the model with insignificant weight gain. Hematological measures revealed no significant differences, except WBC, which was affected by phototherapy (<i>p</i> = 0.037). Blood biochemical measures of creatinine and AST levels were significantly affected by collagenase, while both treatments significantly influence CPK levels (<i>p</i> < 0.001). Histological scores revealed no significant main or interaction effect of both treatment modalities. Effect size estimates for biochemical variables were strong effects while hematological and histological variables demonstrated weak effects. <b>Conclusions:</b> Preconditioning a partially incised tendon with low collagenase and combined with PRP and/or LED therapy may offer therapeutic benefits by enhancing the remodeling phase of tendon repair. Study results validated the rat model, which could be a reliable model for future research to refine treatment as well as the investigational tools protocols. |
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| ISSN: | 2227-9059 |