Antimycobacterial and Nitric Oxide Production Inhibitory Activities of Ocotea notata from Brazilian Restinga

The genus Ocotea (Lauraceae) is distributed mainly in tropical and subtropical regions. Some species of this genus as O. puberula and O. quixos have been described in the literature, showing antibacterial activity. And Ocotea macrophylla showed anti-inflammatory activity with inhibition of COX-1, CO...

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Main Authors: Isabela Francisca Borges Costa, Sanderson Dias Calixto, Marlon Heggdorne de Araujo, Tatiana Ungaretti Paleo Konno, Luzineide Wanderley Tinoco, Denise Oliveira Guimarães, Elena B. Lasunskaia, Ivana Ramos Correa Leal, Michelle Frazão Muzitano
Format: Article
Language:English
Published: Wiley 2015-01-01
Series:The Scientific World Journal
Online Access:http://dx.doi.org/10.1155/2015/947248
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author Isabela Francisca Borges Costa
Sanderson Dias Calixto
Marlon Heggdorne de Araujo
Tatiana Ungaretti Paleo Konno
Luzineide Wanderley Tinoco
Denise Oliveira Guimarães
Elena B. Lasunskaia
Ivana Ramos Correa Leal
Michelle Frazão Muzitano
author_facet Isabela Francisca Borges Costa
Sanderson Dias Calixto
Marlon Heggdorne de Araujo
Tatiana Ungaretti Paleo Konno
Luzineide Wanderley Tinoco
Denise Oliveira Guimarães
Elena B. Lasunskaia
Ivana Ramos Correa Leal
Michelle Frazão Muzitano
author_sort Isabela Francisca Borges Costa
collection DOAJ
description The genus Ocotea (Lauraceae) is distributed mainly in tropical and subtropical regions. Some species of this genus as O. puberula and O. quixos have been described in the literature, showing antibacterial activity. And Ocotea macrophylla showed anti-inflammatory activity with inhibition of COX-1, COX-2, and LOX-5. The purpose of this study was the phytochemical investigation of the plant species Ocotea notata from Restinga Jurubatiba National Park, Macaé, RJ, Brazil, and the search for antimycobacterial fractions and compounds. The crude extract was evaluated for antimycobacterial activity and presented 95.75±2.53% of growth inhibition at 100 µg/mL. Then, it was subjected to a liquid-liquid partition and subsequently was chemically investigated by HPLC, revealing the major presence of flavonoids. In this process the partition fractions hexane, ethyl acetate, and butanol are shown to be promising in the antimycobacterial assay. In addition, ethyl acetate fraction was chromatographed and afforded two flavonoids identified by MS and NMR as afzelin and isoquercitrin. The isolated flavonoids afzelin and isoquercitrin were evaluated for their antimycobacterial activity and for their ability to inhibit NO production by macrophages stimulated by LPS; both flavonoids isoquercitrin (Acet22) and afzelin (Acet32) were able to inhibit the production of NO by macrophages. The calculated IC50 of Acet22 and Acet32 was 1.03 and 0.85 µg/mL, respectively.
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spelling doaj-art-bc730fa5819d47ea93d200f1948e60e22025-08-20T03:23:19ZengWileyThe Scientific World Journal2356-61401537-744X2015-01-01201510.1155/2015/947248947248Antimycobacterial and Nitric Oxide Production Inhibitory Activities of Ocotea notata from Brazilian RestingaIsabela Francisca Borges Costa0Sanderson Dias Calixto1Marlon Heggdorne de Araujo2Tatiana Ungaretti Paleo Konno3Luzineide Wanderley Tinoco4Denise Oliveira Guimarães5Elena B. Lasunskaia6Ivana Ramos Correa Leal7Michelle Frazão Muzitano8Laboratório de Produtos Naturais (LaProN), Curso de Farmácia, Universidade Federal do Rio de Janeiro, Campus Macaé, Polo Novo Cavaleiro-IMMT, R. Aluísio da Silva Gomes 50, 27930-560 Macaé, RJ, BrazilLaboratório de Biologia do Reconhecer, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, 28013-602 Campos dos Goytacazes, RJ, BrazilLaboratório de Produtos Naturais (LaProN), Curso de Farmácia, Universidade Federal do Rio de Janeiro, Campus Macaé, Polo Novo Cavaleiro-IMMT, R. Aluísio da Silva Gomes 50, 27930-560 Macaé, RJ, BrazilNúcleo de Estudos em Ecologia e Desenvolvimento Sócio-Ambiental de Macaé, Universidade Federal do Rio de Janeiro, 27910-970 Macaé, RJ, BrazilInstituto de Pesquisas de Produtos Naturais, Universidade Federal do Rio de Janeiro, 21941-902 Rio de Janeiro, RJ, BrazilLaboratório de Produtos Naturais (LaProN), Curso de Farmácia, Universidade Federal do Rio de Janeiro, Campus Macaé, Polo Novo Cavaleiro-IMMT, R. Aluísio da Silva Gomes 50, 27930-560 Macaé, RJ, BrazilLaboratório de Biologia do Reconhecer, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, 28013-602 Campos dos Goytacazes, RJ, BrazilLaboratório de Produtos Naturais (LaProN), Curso de Farmácia, Universidade Federal do Rio de Janeiro, Campus Macaé, Polo Novo Cavaleiro-IMMT, R. Aluísio da Silva Gomes 50, 27930-560 Macaé, RJ, BrazilLaboratório de Produtos Naturais (LaProN), Curso de Farmácia, Universidade Federal do Rio de Janeiro, Campus Macaé, Polo Novo Cavaleiro-IMMT, R. Aluísio da Silva Gomes 50, 27930-560 Macaé, RJ, BrazilThe genus Ocotea (Lauraceae) is distributed mainly in tropical and subtropical regions. Some species of this genus as O. puberula and O. quixos have been described in the literature, showing antibacterial activity. And Ocotea macrophylla showed anti-inflammatory activity with inhibition of COX-1, COX-2, and LOX-5. The purpose of this study was the phytochemical investigation of the plant species Ocotea notata from Restinga Jurubatiba National Park, Macaé, RJ, Brazil, and the search for antimycobacterial fractions and compounds. The crude extract was evaluated for antimycobacterial activity and presented 95.75±2.53% of growth inhibition at 100 µg/mL. Then, it was subjected to a liquid-liquid partition and subsequently was chemically investigated by HPLC, revealing the major presence of flavonoids. In this process the partition fractions hexane, ethyl acetate, and butanol are shown to be promising in the antimycobacterial assay. In addition, ethyl acetate fraction was chromatographed and afforded two flavonoids identified by MS and NMR as afzelin and isoquercitrin. The isolated flavonoids afzelin and isoquercitrin were evaluated for their antimycobacterial activity and for their ability to inhibit NO production by macrophages stimulated by LPS; both flavonoids isoquercitrin (Acet22) and afzelin (Acet32) were able to inhibit the production of NO by macrophages. The calculated IC50 of Acet22 and Acet32 was 1.03 and 0.85 µg/mL, respectively.http://dx.doi.org/10.1155/2015/947248
spellingShingle Isabela Francisca Borges Costa
Sanderson Dias Calixto
Marlon Heggdorne de Araujo
Tatiana Ungaretti Paleo Konno
Luzineide Wanderley Tinoco
Denise Oliveira Guimarães
Elena B. Lasunskaia
Ivana Ramos Correa Leal
Michelle Frazão Muzitano
Antimycobacterial and Nitric Oxide Production Inhibitory Activities of Ocotea notata from Brazilian Restinga
The Scientific World Journal
title Antimycobacterial and Nitric Oxide Production Inhibitory Activities of Ocotea notata from Brazilian Restinga
title_full Antimycobacterial and Nitric Oxide Production Inhibitory Activities of Ocotea notata from Brazilian Restinga
title_fullStr Antimycobacterial and Nitric Oxide Production Inhibitory Activities of Ocotea notata from Brazilian Restinga
title_full_unstemmed Antimycobacterial and Nitric Oxide Production Inhibitory Activities of Ocotea notata from Brazilian Restinga
title_short Antimycobacterial and Nitric Oxide Production Inhibitory Activities of Ocotea notata from Brazilian Restinga
title_sort antimycobacterial and nitric oxide production inhibitory activities of ocotea notata from brazilian restinga
url http://dx.doi.org/10.1155/2015/947248
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