Strain sharing and mobile genetic elements shape the interconnected resistomes of Campylobacter coli in Brazil

Abstract Background Campylobacter coli is a ubiquitous commensal in the gut of birds and mammals. It causes acute gastroenteritis in humans when contaminated livestock meat is consumed and can be fatal in vulnerable individuals. Here, we aim to characterize the population genomic structure and antim...

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Main Authors: Ana Beatriz Garcez Buiatte, Leticia Roberta Martins Costa, Stephanie S. R. Souza, Nicole I. Zac Soligno, Roberta Torres de Melo, Paulo Marcel Armendaris, Daise Aparecida Rossi, Cheryl P. Andam
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Language:English
Published: BMC 2025-07-01
Series:BMC Biology
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Online Access:https://doi.org/10.1186/s12915-025-02283-y
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author Ana Beatriz Garcez Buiatte
Leticia Roberta Martins Costa
Stephanie S. R. Souza
Nicole I. Zac Soligno
Roberta Torres de Melo
Paulo Marcel Armendaris
Daise Aparecida Rossi
Cheryl P. Andam
author_facet Ana Beatriz Garcez Buiatte
Leticia Roberta Martins Costa
Stephanie S. R. Souza
Nicole I. Zac Soligno
Roberta Torres de Melo
Paulo Marcel Armendaris
Daise Aparecida Rossi
Cheryl P. Andam
author_sort Ana Beatriz Garcez Buiatte
collection DOAJ
description Abstract Background Campylobacter coli is a ubiquitous commensal in the gut of birds and mammals. It causes acute gastroenteritis in humans when contaminated livestock meat is consumed and can be fatal in vulnerable individuals. Here, we aim to characterize the population genomic structure and antimicrobial resistance (AMR) dissemination of C. coli in Brazil contextualized against a South American background. Results We analyzed 32 newly sequenced short-read genomes from Brazil and 158 previously sequenced genomes from different locations in South America. Clonal complex CC828, which is known to be predominant in agriculture and human diseases, accounted for 82.4% of the Brazilian genomes (n = 108). We identified six mutations, 15 acquired genes, and one operon (cmeABC) that were associated with AMR in the Brazilian population. Six AMR determinants (aad9, aph(3')-IIIa, gyrA T86I, 23S A2075G, blaOXA-460, tet(O)) displayed co-occurrence albeit at different paired combinations and probabilities. The Brazilian genomes showed close phylogenetic relationship with those from Chile, Ecuador, and Peru. Across the four countries, AMR determinants associated with putative plasmids or transposable elements included those related to aminoglycosides, streptothricin, tetracyclines, and multidrug resistance. Conclusions The widespread dissemination of CC828 and their mobile genetic elements shape AMR distribution in C. coli across Brazil. A long-term epidemiological and genomic surveillance system in the country will be useful to determine how campylobacteriosis can be controlled effectively and the scale at which interventions need to be enforced.
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spelling doaj-art-bc6a1a4b71b7402794b37fd6b57d7f752025-08-20T03:45:36ZengBMCBMC Biology1741-70072025-07-0123111610.1186/s12915-025-02283-yStrain sharing and mobile genetic elements shape the interconnected resistomes of Campylobacter coli in BrazilAna Beatriz Garcez Buiatte0Leticia Roberta Martins Costa1Stephanie S. R. Souza2Nicole I. Zac Soligno3Roberta Torres de Melo4Paulo Marcel Armendaris5Daise Aparecida Rossi6Cheryl P. Andam7Molecular Epidemiology Laboratory, Federal University of UberlândiaMolecular Epidemiology Laboratory, Federal University of UberlândiaDepartment of Biological Sciences, University at Albany, State University of New YorkDepartment of Biological Sciences, University at Albany, State University of New YorkMolecular Epidemiology Laboratory, Federal University of UberlândiaFederal Agriculture Defense Laboratory/RS - LFDA/RSMolecular Epidemiology Laboratory, Federal University of UberlândiaDepartment of Biological Sciences, University at Albany, State University of New YorkAbstract Background Campylobacter coli is a ubiquitous commensal in the gut of birds and mammals. It causes acute gastroenteritis in humans when contaminated livestock meat is consumed and can be fatal in vulnerable individuals. Here, we aim to characterize the population genomic structure and antimicrobial resistance (AMR) dissemination of C. coli in Brazil contextualized against a South American background. Results We analyzed 32 newly sequenced short-read genomes from Brazil and 158 previously sequenced genomes from different locations in South America. Clonal complex CC828, which is known to be predominant in agriculture and human diseases, accounted for 82.4% of the Brazilian genomes (n = 108). We identified six mutations, 15 acquired genes, and one operon (cmeABC) that were associated with AMR in the Brazilian population. Six AMR determinants (aad9, aph(3')-IIIa, gyrA T86I, 23S A2075G, blaOXA-460, tet(O)) displayed co-occurrence albeit at different paired combinations and probabilities. The Brazilian genomes showed close phylogenetic relationship with those from Chile, Ecuador, and Peru. Across the four countries, AMR determinants associated with putative plasmids or transposable elements included those related to aminoglycosides, streptothricin, tetracyclines, and multidrug resistance. Conclusions The widespread dissemination of CC828 and their mobile genetic elements shape AMR distribution in C. coli across Brazil. A long-term epidemiological and genomic surveillance system in the country will be useful to determine how campylobacteriosis can be controlled effectively and the scale at which interventions need to be enforced.https://doi.org/10.1186/s12915-025-02283-yCampylobacter coliGenomeAntimicrobial resistanceMobile genetic elementsPopulation genomics
spellingShingle Ana Beatriz Garcez Buiatte
Leticia Roberta Martins Costa
Stephanie S. R. Souza
Nicole I. Zac Soligno
Roberta Torres de Melo
Paulo Marcel Armendaris
Daise Aparecida Rossi
Cheryl P. Andam
Strain sharing and mobile genetic elements shape the interconnected resistomes of Campylobacter coli in Brazil
BMC Biology
Campylobacter coli
Genome
Antimicrobial resistance
Mobile genetic elements
Population genomics
title Strain sharing and mobile genetic elements shape the interconnected resistomes of Campylobacter coli in Brazil
title_full Strain sharing and mobile genetic elements shape the interconnected resistomes of Campylobacter coli in Brazil
title_fullStr Strain sharing and mobile genetic elements shape the interconnected resistomes of Campylobacter coli in Brazil
title_full_unstemmed Strain sharing and mobile genetic elements shape the interconnected resistomes of Campylobacter coli in Brazil
title_short Strain sharing and mobile genetic elements shape the interconnected resistomes of Campylobacter coli in Brazil
title_sort strain sharing and mobile genetic elements shape the interconnected resistomes of campylobacter coli in brazil
topic Campylobacter coli
Genome
Antimicrobial resistance
Mobile genetic elements
Population genomics
url https://doi.org/10.1186/s12915-025-02283-y
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