Could kDNA-PCR in Peripheral Blood Replace the Examination of Bone Marrow for the Diagnosis of Visceral Leishmaniasis?
The aim of this study was to evaluate whether the molecular (kDNA-PCR) and parasitological diagnosis in peripheral blood (PB) could replace the invasive and painful bone marrow collection (BM) in the diagnosis of visceral leishmaniasis (VL). PB from suspected VL patients was evaluated by parasitolog...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Parasitology Research |
| Online Access: | http://dx.doi.org/10.1155/2016/1084353 |
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| author | Natalia Souza de Godoy Marcos Luiz Alves Andrino Regina Maia de Souza Erika Gakiya Valdir Sabbaga Amato José Ângelo Lauletta Lindoso Lucia Maria Almeida Braz |
| author_facet | Natalia Souza de Godoy Marcos Luiz Alves Andrino Regina Maia de Souza Erika Gakiya Valdir Sabbaga Amato José Ângelo Lauletta Lindoso Lucia Maria Almeida Braz |
| author_sort | Natalia Souza de Godoy |
| collection | DOAJ |
| description | The aim of this study was to evaluate whether the molecular (kDNA-PCR) and parasitological diagnosis in peripheral blood (PB) could replace the invasive and painful bone marrow collection (BM) in the diagnosis of visceral leishmaniasis (VL). PB from suspected VL patients was evaluated by parasitological and molecular techniques using as the gold standard (GS) a combination of clinical, epidemiological, and immunochromatographic test (PB-rK39) results and parasitological examination of BM. Based on the GS, 38 samples from 32 patients were grouped: Group 1, 20 samples of VL cases, and Group 2, 18 samples of non-VL cases. In order to evaluate the parasitological and molecular techniques in PB, the samples were examined. From Group 1, PB kDNA-PCR was positive in 20 samples and in 19 of 20 in BM kDNA-PCR examination. However, the parasitological examination of buffy coat was insensitive, being able to detect only 4 cases from Group 1. All samples from Group 2 were negative. We concluded that, for the diagnosis of visceral leishmaniasis, the parasitological examination of peripheral blood was not useful; however, molecular diagnosis by kDNA-PCR, performed in peripheral blood, could be useful to replace the parasitological examination of bone marrow. |
| format | Article |
| id | doaj-art-bc693ab4d198480e8caedfcf0a385ea7 |
| institution | Kabale University |
| issn | 2090-0023 2090-0031 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Parasitology Research |
| spelling | doaj-art-bc693ab4d198480e8caedfcf0a385ea72025-02-03T05:44:04ZengWileyJournal of Parasitology Research2090-00232090-00312016-01-01201610.1155/2016/10843531084353Could kDNA-PCR in Peripheral Blood Replace the Examination of Bone Marrow for the Diagnosis of Visceral Leishmaniasis?Natalia Souza de Godoy0Marcos Luiz Alves Andrino1Regina Maia de Souza2Erika Gakiya3Valdir Sabbaga Amato4José Ângelo Lauletta Lindoso5Lucia Maria Almeida Braz6Laboratório de Investigação Médica, Parasitologia LIM 46 do Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo (HCFMUSP), Avenida Dr. Enéas Carvalho de Aguiar 470/500, 05403-000 São Paulo, SP, BrazilLaboratório de Investigação Médica, Parasitologia LIM 46 do Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo (HCFMUSP), Avenida Dr. Enéas Carvalho de Aguiar 470/500, 05403-000 São Paulo, SP, BrazilLaboratório de Investigação Médica, Parasitologia LIM 46 do Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo (HCFMUSP), Avenida Dr. Enéas Carvalho de Aguiar 470/500, 05403-000 São Paulo, SP, BrazilLaboratório de Investigação Médica, Parasitologia LIM 46 do Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo (HCFMUSP), Avenida Dr. Enéas Carvalho de Aguiar 470/500, 05403-000 São Paulo, SP, BrazilLaboratório de Investigação Médica, Parasitologia LIM 46 do Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo (HCFMUSP), Avenida Dr. Enéas Carvalho de Aguiar 470/500, 05403-000 São Paulo, SP, BrazilLaboratório de Soroepidemiologia e Imunobiologia, IMTSP-USP, Avenida Dr. Enéas Carvalho de Aguiar 470/500, 05403-000 São Paulo, SP, BrazilLaboratório de Parasitologia, Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo (IMTSP-USP), Avenida Dr. Enéas Carvalho de Aguiar 470/500, 05403-000 São Paulo, SP, BrazilThe aim of this study was to evaluate whether the molecular (kDNA-PCR) and parasitological diagnosis in peripheral blood (PB) could replace the invasive and painful bone marrow collection (BM) in the diagnosis of visceral leishmaniasis (VL). PB from suspected VL patients was evaluated by parasitological and molecular techniques using as the gold standard (GS) a combination of clinical, epidemiological, and immunochromatographic test (PB-rK39) results and parasitological examination of BM. Based on the GS, 38 samples from 32 patients were grouped: Group 1, 20 samples of VL cases, and Group 2, 18 samples of non-VL cases. In order to evaluate the parasitological and molecular techniques in PB, the samples were examined. From Group 1, PB kDNA-PCR was positive in 20 samples and in 19 of 20 in BM kDNA-PCR examination. However, the parasitological examination of buffy coat was insensitive, being able to detect only 4 cases from Group 1. All samples from Group 2 were negative. We concluded that, for the diagnosis of visceral leishmaniasis, the parasitological examination of peripheral blood was not useful; however, molecular diagnosis by kDNA-PCR, performed in peripheral blood, could be useful to replace the parasitological examination of bone marrow.http://dx.doi.org/10.1155/2016/1084353 |
| spellingShingle | Natalia Souza de Godoy Marcos Luiz Alves Andrino Regina Maia de Souza Erika Gakiya Valdir Sabbaga Amato José Ângelo Lauletta Lindoso Lucia Maria Almeida Braz Could kDNA-PCR in Peripheral Blood Replace the Examination of Bone Marrow for the Diagnosis of Visceral Leishmaniasis? Journal of Parasitology Research |
| title | Could kDNA-PCR in Peripheral Blood Replace the Examination of Bone Marrow for the Diagnosis of Visceral Leishmaniasis? |
| title_full | Could kDNA-PCR in Peripheral Blood Replace the Examination of Bone Marrow for the Diagnosis of Visceral Leishmaniasis? |
| title_fullStr | Could kDNA-PCR in Peripheral Blood Replace the Examination of Bone Marrow for the Diagnosis of Visceral Leishmaniasis? |
| title_full_unstemmed | Could kDNA-PCR in Peripheral Blood Replace the Examination of Bone Marrow for the Diagnosis of Visceral Leishmaniasis? |
| title_short | Could kDNA-PCR in Peripheral Blood Replace the Examination of Bone Marrow for the Diagnosis of Visceral Leishmaniasis? |
| title_sort | could kdna pcr in peripheral blood replace the examination of bone marrow for the diagnosis of visceral leishmaniasis |
| url | http://dx.doi.org/10.1155/2016/1084353 |
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