Drinking Water and Biofilm as Sources of Antimicrobial Resistance in Free-Range Organic Broiler Farms
Drinking water distribution systems (DWDSs) represent an ideal environment for biofilm formation, which can harbor pathogenic and antimicrobial-resistant bacteria. This study aimed to assess longitudinally the microbial community composition and antimicrobial resistance (AMR), as determined by 16S r...
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MDPI AG
2024-08-01
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| author | Alessandra Piccirillo Roberta Tolosi Lapo Mughini-Gras Jannigje G. Kers Andrea Laconi |
| author_facet | Alessandra Piccirillo Roberta Tolosi Lapo Mughini-Gras Jannigje G. Kers Andrea Laconi |
| author_sort | Alessandra Piccirillo |
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| description | Drinking water distribution systems (DWDSs) represent an ideal environment for biofilm formation, which can harbor pathogenic and antimicrobial-resistant bacteria. This study aimed to assess longitudinally the microbial community composition and antimicrobial resistance (AMR), as determined by 16S rRNA NGS and qPCR, respectively, in drinking water (DW) and biofilm from DWDSs, as well as faeces, of free-range organic broiler farms. The role of DWDSs in AMR gene (ARG) dissemination within the farm environment and transmission to animals, was also assessed. DW and biofilm microbial communities differed from those of faecal samples. Moreover, potentially pathogenic and opportunistic bacteria (e.g., Staphylococcaceae) were identified in water and biofilms. High prevalence and abundance of ARGs conferring resistance to carbapenems (i.e., <i>bla<sub>NDM</sub></i>), 3rd and 4th generation cephalosporins (i.e., <i>bla<sub>CMY-2</sub></i>), (fluoro)quinolones (i.e., <i>qnrS</i>), and polymyxins (i.e., <i>mcr-3</i> and <i>mcr-5</i>) were detected in DW, biofilm, and faecal samples, which is of concern for both animal and human health. Although other factors (e.g., feed, pests, and wildlife) may contribute to the dissemination of AMR in free-range organic poultry farms, this study indicates that DWDSs can also play a role. |
| format | Article |
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| issn | 2079-6382 |
| language | English |
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| spelling | doaj-art-bc685ad927d3445891d9533db37e90ef2025-08-20T01:56:04ZengMDPI AGAntibiotics2079-63822024-08-0113980810.3390/antibiotics13090808Drinking Water and Biofilm as Sources of Antimicrobial Resistance in Free-Range Organic Broiler FarmsAlessandra Piccirillo0Roberta Tolosi1Lapo Mughini-Gras2Jannigje G. Kers3Andrea Laconi4Department of Comparative Biomedicine and Food Science, University of Padua, Viale dell’Università 16, 35020 Legnaro, ItalyDepartment of Comparative Biomedicine and Food Science, University of Padua, Viale dell’Università 16, 35020 Legnaro, ItalyCenter for Infectious Disease Control, National Institute for Public Health and the Environment (RIVM), Antonie van Leeuwenhoeklaan 9, 3721 BA Bilthoven, The NetherlandsFaculty of Veterinary Medicine, Utrecht University, Yalelaan 2, De Uithof, 3584 CL Utrecht, The NetherlandsDepartment of Comparative Biomedicine and Food Science, University of Padua, Viale dell’Università 16, 35020 Legnaro, ItalyDrinking water distribution systems (DWDSs) represent an ideal environment for biofilm formation, which can harbor pathogenic and antimicrobial-resistant bacteria. This study aimed to assess longitudinally the microbial community composition and antimicrobial resistance (AMR), as determined by 16S rRNA NGS and qPCR, respectively, in drinking water (DW) and biofilm from DWDSs, as well as faeces, of free-range organic broiler farms. The role of DWDSs in AMR gene (ARG) dissemination within the farm environment and transmission to animals, was also assessed. DW and biofilm microbial communities differed from those of faecal samples. Moreover, potentially pathogenic and opportunistic bacteria (e.g., Staphylococcaceae) were identified in water and biofilms. High prevalence and abundance of ARGs conferring resistance to carbapenems (i.e., <i>bla<sub>NDM</sub></i>), 3rd and 4th generation cephalosporins (i.e., <i>bla<sub>CMY-2</sub></i>), (fluoro)quinolones (i.e., <i>qnrS</i>), and polymyxins (i.e., <i>mcr-3</i> and <i>mcr-5</i>) were detected in DW, biofilm, and faecal samples, which is of concern for both animal and human health. Although other factors (e.g., feed, pests, and wildlife) may contribute to the dissemination of AMR in free-range organic poultry farms, this study indicates that DWDSs can also play a role.https://www.mdpi.com/2079-6382/13/9/808AMRresistance geneswaterbiofilmmicrobiotafree-range |
| spellingShingle | Alessandra Piccirillo Roberta Tolosi Lapo Mughini-Gras Jannigje G. Kers Andrea Laconi Drinking Water and Biofilm as Sources of Antimicrobial Resistance in Free-Range Organic Broiler Farms Antibiotics AMR resistance genes water biofilm microbiota free-range |
| title | Drinking Water and Biofilm as Sources of Antimicrobial Resistance in Free-Range Organic Broiler Farms |
| title_full | Drinking Water and Biofilm as Sources of Antimicrobial Resistance in Free-Range Organic Broiler Farms |
| title_fullStr | Drinking Water and Biofilm as Sources of Antimicrobial Resistance in Free-Range Organic Broiler Farms |
| title_full_unstemmed | Drinking Water and Biofilm as Sources of Antimicrobial Resistance in Free-Range Organic Broiler Farms |
| title_short | Drinking Water and Biofilm as Sources of Antimicrobial Resistance in Free-Range Organic Broiler Farms |
| title_sort | drinking water and biofilm as sources of antimicrobial resistance in free range organic broiler farms |
| topic | AMR resistance genes water biofilm microbiota free-range |
| url | https://www.mdpi.com/2079-6382/13/9/808 |
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