Determination of Anther Culture Performance of Some Pepper (Capsicum annuum L.) Genotypes
In this study, anther culture performances of 23 pepper genotypes belonging different types were determined. In disinfection of flower buds, the buds were first kept in 70% ethyl alcohol for 30 seconds and then were waited in 15% sodium hypochlorite solution for 10 minutes. The anthers were cultured...
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Yuzuncu Yil University Faculty of Agriculture
2021-06-01
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| Series: | Yüzüncü Yıl Üniversitesi Tarim Bilimleri Dergisi |
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| author | Didem ATASOY Gökhan BAKTEMUR Hatıra TAŞKIN |
| author_facet | Didem ATASOY Gökhan BAKTEMUR Hatıra TAŞKIN |
| author_sort | Didem ATASOY |
| collection | DOAJ |
| description | In this study, anther culture performances of 23 pepper genotypes belonging different types were determined. In disinfection of flower buds, the buds were first kept in 70% ethyl alcohol for 30 seconds and then were waited in 15% sodium hypochlorite solution for 10 minutes. The anthers were cultured in Murashige and Skoog (MS) nutrient medium containing 30 g L-1 sucrose, 2.5 g L-1 activated charcoal, 15 mg L-1 silver nitrate (AgNO3), 4 mg L-1 1-Naphthaleneacetic acid (NAA), 0.5 mg L-1 6-Benzylaminopurine (BAP) and 6.5 g L-1 agar in the first stage. The anthers were then transferred to MS medium including 30 g L-1 sucrose and 6.5 g L-1 agar and without growth regulators. The embryos obtained were taken to the same nutrient medium (second one). The anthers were cultured at +35ºC and in dark conditions for 2 days as pre-treatment. When the results of the study were evaluated, average embryo number per hundred anthers according to the genotypes ranged from 0.83 to 44.44. The highest ratio was observed in the genotype FT-509 and it was followed by the genotypes FT-508 (23.61 embryos/100 anthers), FT-1181 (23.37 embryos/100 anthers) and FT-905 (22.89 embryos/100 anthers). The genotype FT-1178 showed the lowest performance with 0.83 embryos/100 anthers. All of the embryos developed could transform into plants. Therefore, the average embryo number forming plant per 100 anthers was the same with average embryo number per hundred anthers. The highest average plant number was observed in the genotype FT-508 with 20 plants and it was followed by the genotypes FT-509 (17.5 plants), FT-1181 (16 plants), FT-905 (12 plants), FT-263 (10.5 plants) and FT-507 (8.25 plants). The lowest number of plant was recorded in the genotype FT-1178 with 0.5 plant. |
| format | Article |
| id | doaj-art-bc673c3fb8b94f11b53ea78441a21456 |
| institution | OA Journals |
| issn | 1308-7576 1308-7584 |
| language | English |
| publishDate | 2021-06-01 |
| publisher | Yuzuncu Yil University Faculty of Agriculture |
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| series | Yüzüncü Yıl Üniversitesi Tarim Bilimleri Dergisi |
| spelling | doaj-art-bc673c3fb8b94f11b53ea78441a214562025-08-20T02:18:33ZengYuzuncu Yil University Faculty of AgricultureYüzüncü Yıl Üniversitesi Tarim Bilimleri Dergisi1308-75761308-75842021-06-0131228229310.29133/yyutbd.835106Determination of Anther Culture Performance of Some Pepper (Capsicum annuum L.) GenotypesDidem ATASOYhttps://orcid.org/0000-0003-0659-2943Gökhan BAKTEMURhttps://orcid.org/0000-0002-0362-5108Hatıra TAŞKINhttps://orcid.org/0000-0002-1784-4731In this study, anther culture performances of 23 pepper genotypes belonging different types were determined. In disinfection of flower buds, the buds were first kept in 70% ethyl alcohol for 30 seconds and then were waited in 15% sodium hypochlorite solution for 10 minutes. The anthers were cultured in Murashige and Skoog (MS) nutrient medium containing 30 g L-1 sucrose, 2.5 g L-1 activated charcoal, 15 mg L-1 silver nitrate (AgNO3), 4 mg L-1 1-Naphthaleneacetic acid (NAA), 0.5 mg L-1 6-Benzylaminopurine (BAP) and 6.5 g L-1 agar in the first stage. The anthers were then transferred to MS medium including 30 g L-1 sucrose and 6.5 g L-1 agar and without growth regulators. The embryos obtained were taken to the same nutrient medium (second one). The anthers were cultured at +35ºC and in dark conditions for 2 days as pre-treatment. When the results of the study were evaluated, average embryo number per hundred anthers according to the genotypes ranged from 0.83 to 44.44. The highest ratio was observed in the genotype FT-509 and it was followed by the genotypes FT-508 (23.61 embryos/100 anthers), FT-1181 (23.37 embryos/100 anthers) and FT-905 (22.89 embryos/100 anthers). The genotype FT-1178 showed the lowest performance with 0.83 embryos/100 anthers. All of the embryos developed could transform into plants. Therefore, the average embryo number forming plant per 100 anthers was the same with average embryo number per hundred anthers. The highest average plant number was observed in the genotype FT-508 with 20 plants and it was followed by the genotypes FT-509 (17.5 plants), FT-1181 (16 plants), FT-905 (12 plants), FT-263 (10.5 plants) and FT-507 (8.25 plants). The lowest number of plant was recorded in the genotype FT-1178 with 0.5 plant.anther culturepeppercapsicum annuumgenotype |
| spellingShingle | Didem ATASOY Gökhan BAKTEMUR Hatıra TAŞKIN Determination of Anther Culture Performance of Some Pepper (Capsicum annuum L.) Genotypes Yüzüncü Yıl Üniversitesi Tarim Bilimleri Dergisi anther culture pepper capsicum annuum genotype |
| title | Determination of Anther Culture Performance of Some Pepper (Capsicum annuum L.) Genotypes |
| title_full | Determination of Anther Culture Performance of Some Pepper (Capsicum annuum L.) Genotypes |
| title_fullStr | Determination of Anther Culture Performance of Some Pepper (Capsicum annuum L.) Genotypes |
| title_full_unstemmed | Determination of Anther Culture Performance of Some Pepper (Capsicum annuum L.) Genotypes |
| title_short | Determination of Anther Culture Performance of Some Pepper (Capsicum annuum L.) Genotypes |
| title_sort | determination of anther culture performance of some pepper capsicum annuum l genotypes |
| topic | anther culture pepper capsicum annuum genotype |
| work_keys_str_mv | AT didematasoy determinationofanthercultureperformanceofsomepeppercapsicumannuumlgenotypes AT gokhanbaktemur determinationofanthercultureperformanceofsomepeppercapsicumannuumlgenotypes AT hatırataskin determinationofanthercultureperformanceofsomepeppercapsicumannuumlgenotypes |