The Role of Moringa oleifera- Ifalmin® Formulation in Regulation of B220+IgM+ and B220+IgG+ in Diabetic Mice
It has been known that the immunoglobulin levels were altered in diabetes mellitus (DM) conditions. This study aimed to evaluate the levels of immunoglobulins in DM mice after the administration of Moringa oleifera-Ifalmin® formulation (MI). Streptozotocin, at a dose of 145 mg.kg-1, was injected int...
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University of Brawijaya
2020-02-01
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| Series: | Journal of Experimental Life Science |
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| Online Access: | https://jels.ub.ac.id/index.php/jels/article/view/385 |
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| author | Wahyu Isnia Adharini Ruri Vivian Nilamsari Noviana Dwi Lestari Nashi Widodo Muhaimin Rifa'i |
| author_facet | Wahyu Isnia Adharini Ruri Vivian Nilamsari Noviana Dwi Lestari Nashi Widodo Muhaimin Rifa'i |
| author_sort | Wahyu Isnia Adharini |
| collection | DOAJ |
| description | It has been known that the immunoglobulin levels were altered in diabetes mellitus (DM) conditions. This study aimed to evaluate the levels of immunoglobulins in DM mice after the administration of Moringa oleifera-Ifalmin® formulation (MI). Streptozotocin, at a dose of 145 mg.kg-1, was injected intraperitoneally to experimental mice to obtain diabetic mice. The groups were divided into normal mice, diabetic mice without treatment, diabetic mice with metformin treatment (307.5 mg.kg-1 BW), and diabetic mice with MI treatment at dose 1 (M:I= 800 mg.kg-1 BW: 800 mg.kg-1 BW), dose 2 (M:I= 615 mg.kg-1 BW: 615 mg.kg-1 BW), and dose 3 (M:I= 800 mg.kg-1 BW: 615 mg.kg-1 BW). Mice were orally treated by MI for 14 days. Subsequently, the levels of immunoglobulin IgM and IgG were evaluated using flow cytometry analysis. IgM and IgG levels were significantly lower in the DM group than the normal group. These results indicated that DM altered immunoglobulin levels. MI treatment for 14 days significantly increased the number of IgM and IgG at the level equivalent to the normal group and significantly different as compared to the DM group. Based on the results, MI can be used as an immunomodulatory agent in humoral immunity through the precise regulation of IgM and IgG. |
| format | Article |
| id | doaj-art-bc62c063fd514ff1b5eb8551c37a31b7 |
| institution | Kabale University |
| issn | 2087-2852 2338-1655 |
| language | English |
| publishDate | 2020-02-01 |
| publisher | University of Brawijaya |
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| series | Journal of Experimental Life Science |
| spelling | doaj-art-bc62c063fd514ff1b5eb8551c37a31b72025-08-20T03:49:45ZengUniversity of BrawijayaJournal of Experimental Life Science2087-28522338-16552020-02-011014348https://doi.org/10.21776/ub.jels.2019.010.01.08The Role of Moringa oleifera- Ifalmin® Formulation in Regulation of B220+IgM+ and B220+IgG+ in Diabetic MiceWahyu Isnia Adharini0Ruri Vivian Nilamsari1Noviana Dwi Lestari2Nashi Widodo3Muhaimin Rifa'i4Universitas BrawijayaUniversitas BrawijayaUniversitas BrawijayaUniversitas BrawijayaUniversitas BrawijayaIt has been known that the immunoglobulin levels were altered in diabetes mellitus (DM) conditions. This study aimed to evaluate the levels of immunoglobulins in DM mice after the administration of Moringa oleifera-Ifalmin® formulation (MI). Streptozotocin, at a dose of 145 mg.kg-1, was injected intraperitoneally to experimental mice to obtain diabetic mice. The groups were divided into normal mice, diabetic mice without treatment, diabetic mice with metformin treatment (307.5 mg.kg-1 BW), and diabetic mice with MI treatment at dose 1 (M:I= 800 mg.kg-1 BW: 800 mg.kg-1 BW), dose 2 (M:I= 615 mg.kg-1 BW: 615 mg.kg-1 BW), and dose 3 (M:I= 800 mg.kg-1 BW: 615 mg.kg-1 BW). Mice were orally treated by MI for 14 days. Subsequently, the levels of immunoglobulin IgM and IgG were evaluated using flow cytometry analysis. IgM and IgG levels were significantly lower in the DM group than the normal group. These results indicated that DM altered immunoglobulin levels. MI treatment for 14 days significantly increased the number of IgM and IgG at the level equivalent to the normal group and significantly different as compared to the DM group. Based on the results, MI can be used as an immunomodulatory agent in humoral immunity through the precise regulation of IgM and IgG.https://jels.ub.ac.id/index.php/jels/article/view/385diabetes mellitusifalminâ®immunoglobulinimmunomodulatormoringa oleifera |
| spellingShingle | Wahyu Isnia Adharini Ruri Vivian Nilamsari Noviana Dwi Lestari Nashi Widodo Muhaimin Rifa'i The Role of Moringa oleifera- Ifalmin® Formulation in Regulation of B220+IgM+ and B220+IgG+ in Diabetic Mice Journal of Experimental Life Science diabetes mellitus ifalminâ® immunoglobulin immunomodulator moringa oleifera |
| title | The Role of Moringa oleifera- Ifalmin® Formulation in Regulation of B220+IgM+ and B220+IgG+ in Diabetic Mice |
| title_full | The Role of Moringa oleifera- Ifalmin® Formulation in Regulation of B220+IgM+ and B220+IgG+ in Diabetic Mice |
| title_fullStr | The Role of Moringa oleifera- Ifalmin® Formulation in Regulation of B220+IgM+ and B220+IgG+ in Diabetic Mice |
| title_full_unstemmed | The Role of Moringa oleifera- Ifalmin® Formulation in Regulation of B220+IgM+ and B220+IgG+ in Diabetic Mice |
| title_short | The Role of Moringa oleifera- Ifalmin® Formulation in Regulation of B220+IgM+ and B220+IgG+ in Diabetic Mice |
| title_sort | role of moringa oleifera ifalmin r formulation in regulation of b220 igm and b220 igg in diabetic mice |
| topic | diabetes mellitus ifalminâ® immunoglobulin immunomodulator moringa oleifera |
| url | https://jels.ub.ac.id/index.php/jels/article/view/385 |
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