Inflammation-Related MicroRNAs Are Associated with Plaque Stability Calculated by IVUS in Coronary Heart Disease Patients

Objectives. This study aimed to investigate the association between inflammation-related microRNAs (miR-21, 146a, 155) and the plaque stability in coronary artery disease patients. Methods. The expression of miR-21, 146a, and 155 was measured by real-time PCR in 310 consecutive patients. The level o...

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Main Authors: Guo-fu Zhu, Tianshu Chu, Zhimin Ruan, Mingguo Zhang, Mingli Zhou, Qian Zhang, Ran Zhang, Liyong Wu
Format: Article
Language:English
Published: Wiley 2019-01-01
Series:Journal of Interventional Cardiology
Online Access:http://dx.doi.org/10.1155/2019/9723129
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author Guo-fu Zhu
Tianshu Chu
Zhimin Ruan
Mingguo Zhang
Mingli Zhou
Qian Zhang
Ran Zhang
Liyong Wu
author_facet Guo-fu Zhu
Tianshu Chu
Zhimin Ruan
Mingguo Zhang
Mingli Zhou
Qian Zhang
Ran Zhang
Liyong Wu
author_sort Guo-fu Zhu
collection DOAJ
description Objectives. This study aimed to investigate the association between inflammation-related microRNAs (miR-21, 146a, 155) and the plaque stability in coronary artery disease patients. Methods. The expression of miR-21, 146a, and 155 was measured by real-time PCR in 310 consecutive patients. The level of hs-CRP, IL-6, and IL-8 was measured by ELISA. The plaque stability of coronary stenotic lesions was evaluated with intravascular ultrasound (IVUS). Results. (1) The levels of hs-CRP, IL-6, and IL-8 were significantly increased in the UAP and AMI groups compared with the CPS group (P<0.01). (2) The expression of miR-21 and miR-146a in peripheral blood mononuclear cells (PBMCs) and plasma was significantly higher in CAD patients compared with non-CAD patients, whereas the miR-155 expression in PBMCs and plasma was significantly lower in patients with CAD. (3) The miR-21 expression in PBMCs was higher in UAP and AMI groups compared with CPS group. The miR-146a expression in PBMCs was higher in SAP, UAP, and AMI groups than in CPS group. Although the level of miR-155 in PBMCs was lower in SAP, UAP, and AMI groups than in CPS group. The expression patterns of miR-21, miR-146a, and miR-155 in plasma were consistent with those of PBMCs. (4) The expressions of miR-21 and miR-146a in PBMCs and plasma were significantly higher in the vulnerable plaque group than those in stable plaque group. While miR-155 in PBMCs and plasma was significantly lower in vulnerable plaque group compared with stable plaque group. (5) The levels of miR-21 and miR-146a in PBMCs and plasma were significantly higher in soft plaque group than in fibrous plaque group and calcified plaque group. However, miR-155 in PBMCs and plasma was significantly lower in soft plaque group. Conclusions. The expression of miR-21 and miR-146a are associated with the plaque stability in coronary stenotic lesions, whereas miR-155 expression is inversely associated with the plaque stability.
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spelling doaj-art-bc3baa5140c841a58ddb301179b6c4a92025-08-20T02:18:42ZengWileyJournal of Interventional Cardiology0896-43271540-81832019-01-01201910.1155/2019/97231299723129Inflammation-Related MicroRNAs Are Associated with Plaque Stability Calculated by IVUS in Coronary Heart Disease PatientsGuo-fu Zhu0Tianshu Chu1Zhimin Ruan2Mingguo Zhang3Mingli Zhou4Qian Zhang5Ran Zhang6Liyong Wu7Department of Cardiology, The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650101, ChinaDepartment of Cardiology, The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650101, ChinaDepartment of Cardiology, The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650101, ChinaDepartment of Cardiology, The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650101, ChinaDepartment of Cardiology, The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650101, ChinaDepartment of Cardiology, The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650101, ChinaDepartment of Cardiology, The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650101, ChinaDepartment of Cardiology, The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650101, ChinaObjectives. This study aimed to investigate the association between inflammation-related microRNAs (miR-21, 146a, 155) and the plaque stability in coronary artery disease patients. Methods. The expression of miR-21, 146a, and 155 was measured by real-time PCR in 310 consecutive patients. The level of hs-CRP, IL-6, and IL-8 was measured by ELISA. The plaque stability of coronary stenotic lesions was evaluated with intravascular ultrasound (IVUS). Results. (1) The levels of hs-CRP, IL-6, and IL-8 were significantly increased in the UAP and AMI groups compared with the CPS group (P<0.01). (2) The expression of miR-21 and miR-146a in peripheral blood mononuclear cells (PBMCs) and plasma was significantly higher in CAD patients compared with non-CAD patients, whereas the miR-155 expression in PBMCs and plasma was significantly lower in patients with CAD. (3) The miR-21 expression in PBMCs was higher in UAP and AMI groups compared with CPS group. The miR-146a expression in PBMCs was higher in SAP, UAP, and AMI groups than in CPS group. Although the level of miR-155 in PBMCs was lower in SAP, UAP, and AMI groups than in CPS group. The expression patterns of miR-21, miR-146a, and miR-155 in plasma were consistent with those of PBMCs. (4) The expressions of miR-21 and miR-146a in PBMCs and plasma were significantly higher in the vulnerable plaque group than those in stable plaque group. While miR-155 in PBMCs and plasma was significantly lower in vulnerable plaque group compared with stable plaque group. (5) The levels of miR-21 and miR-146a in PBMCs and plasma were significantly higher in soft plaque group than in fibrous plaque group and calcified plaque group. However, miR-155 in PBMCs and plasma was significantly lower in soft plaque group. Conclusions. The expression of miR-21 and miR-146a are associated with the plaque stability in coronary stenotic lesions, whereas miR-155 expression is inversely associated with the plaque stability.http://dx.doi.org/10.1155/2019/9723129
spellingShingle Guo-fu Zhu
Tianshu Chu
Zhimin Ruan
Mingguo Zhang
Mingli Zhou
Qian Zhang
Ran Zhang
Liyong Wu
Inflammation-Related MicroRNAs Are Associated with Plaque Stability Calculated by IVUS in Coronary Heart Disease Patients
Journal of Interventional Cardiology
title Inflammation-Related MicroRNAs Are Associated with Plaque Stability Calculated by IVUS in Coronary Heart Disease Patients
title_full Inflammation-Related MicroRNAs Are Associated with Plaque Stability Calculated by IVUS in Coronary Heart Disease Patients
title_fullStr Inflammation-Related MicroRNAs Are Associated with Plaque Stability Calculated by IVUS in Coronary Heart Disease Patients
title_full_unstemmed Inflammation-Related MicroRNAs Are Associated with Plaque Stability Calculated by IVUS in Coronary Heart Disease Patients
title_short Inflammation-Related MicroRNAs Are Associated with Plaque Stability Calculated by IVUS in Coronary Heart Disease Patients
title_sort inflammation related micrornas are associated with plaque stability calculated by ivus in coronary heart disease patients
url http://dx.doi.org/10.1155/2019/9723129
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