Ex vivo myo-mechanical assessment of isolated rectus abdominis muscle in pregnancy-specific urinary incontinence: a cross-sectional study nested within the Diamater cohort
Abstract Background Rectus abdominis muscle (RAM) myopathy is the underlying mechanism of pregnancy-specific urinary incontinence (PSUI), which has been shown to be a predictor of long-term urinary incontinence (UI). However, there is limited knowledge about selective ex vivo myo-mechanical assessme...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-03-01
|
| Series: | BioMedical Engineering OnLine |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12938-025-01366-9 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850207861910536192 |
|---|---|
| author | Carolina N. F. Carvalho David R. A. Reyes Juliana F. Floriano Sofia B. C. V. Quiroz Rafael G. Oliveira Fernanda C. B. Alves Gabriela A. Garcia Luana F. Iamundo Adriely B. M. Magyori Caroline B. Prudencio Patricia S. Rossignoli Cristiane R. Pedroni Raghavendra L. S. Hallur Iracema M. P. Calderon Angélica M. P. Barbosa Marilza V. C. Rudge The Diamater Study Group |
| author_facet | Carolina N. F. Carvalho David R. A. Reyes Juliana F. Floriano Sofia B. C. V. Quiroz Rafael G. Oliveira Fernanda C. B. Alves Gabriela A. Garcia Luana F. Iamundo Adriely B. M. Magyori Caroline B. Prudencio Patricia S. Rossignoli Cristiane R. Pedroni Raghavendra L. S. Hallur Iracema M. P. Calderon Angélica M. P. Barbosa Marilza V. C. Rudge The Diamater Study Group |
| author_sort | Carolina N. F. Carvalho |
| collection | DOAJ |
| description | Abstract Background Rectus abdominis muscle (RAM) myopathy is the underlying mechanism of pregnancy-specific urinary incontinence (PSUI), which has been shown to be a predictor of long-term urinary incontinence (UI). However, there is limited knowledge about selective ex vivo myo-mechanical assessment (MMA) of the RAM in healthy pregnant women, particularly those with PSUI. Results Our analysis revealed no specific quantitative parameters from the window analysis or contractile response of the RAM in ex vivo MMA. A gradual and consistent decline was predominantly observed in the peak and strength parameters for both groups. The qualitative contractile response analysis of the RAM using peak and strength parameters revealed three distinct behaviors under electrical stimulation: a progressive decrease in strength, sudden muscular arrest, and asynchrony with erratic fluctuations. In-depth quantitative analysis of the RAM ex vivo did not yield substantial differences between the two groups. However, qualitative analysis proved valuable in distinguishing the groups, revealing a tendency toward the loss of the progressive peak declines observed in the control group. Conclusion This study highlights the complexity of RAM myopathy in the context of PSUI and its potential impact on UI outcomes. Method In this cross-sectional study nested within a cohort, we examined ex vivo myography assessments in 87 mothers, comprising 48 with PSUI and 39 without PSUI, all of whom exhibited normal glucose tolerance. General data were extracted from our database, whereas RAM samples were collected during C-section for qualitative and quantitative ex vivo myography analysis, with a focus on initial and final baseline measurements, peak responses, strength, and duration times. |
| format | Article |
| id | doaj-art-bc3b241d309b40ea8e6bffd88e8653e8 |
| institution | OA Journals |
| issn | 1475-925X |
| language | English |
| publishDate | 2025-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BioMedical Engineering OnLine |
| spelling | doaj-art-bc3b241d309b40ea8e6bffd88e8653e82025-08-20T02:10:23ZengBMCBioMedical Engineering OnLine1475-925X2025-03-0124112210.1186/s12938-025-01366-9Ex vivo myo-mechanical assessment of isolated rectus abdominis muscle in pregnancy-specific urinary incontinence: a cross-sectional study nested within the Diamater cohortCarolina N. F. Carvalho0David R. A. Reyes1Juliana F. Floriano2Sofia B. C. V. Quiroz3Rafael G. Oliveira4Fernanda C. B. Alves5Gabriela A. Garcia6Luana F. Iamundo7Adriely B. M. Magyori8Caroline B. Prudencio9Patricia S. Rossignoli10Cristiane R. Pedroni11Raghavendra L. S. Hallur12Iracema M. P. Calderon13Angélica M. P. Barbosa14Marilza V. C. Rudge15The Diamater Study GroupPostgraduate Program On Tocogynecology, Botucatu Medical School (FMB), São Paulo State University (UNESP)Postgraduate Program On Tocogynecology, Botucatu Medical School (FMB), São Paulo State University (UNESP)Postgraduate Program On Tocogynecology, Botucatu Medical School (FMB), São Paulo State University (UNESP)Postgraduate Program On Tocogynecology, Botucatu Medical School (FMB), São Paulo State University (UNESP)Postgraduate Program On Tocogynecology, Botucatu Medical School (FMB), São Paulo State University (UNESP)Postgraduate Program On Tocogynecology, Botucatu Medical School (FMB), São Paulo State University (UNESP)School of Sciences, Postgraduate Program in Materials Science and Technology (POSMAT), São Paulo State University (UNESP)Postgraduate Program On Tocogynecology, Botucatu Medical School (FMB), São Paulo State University (UNESP)Postgraduate Program On Tocogynecology, Botucatu Medical School (FMB), São Paulo State University (UNESP)Postgraduate Program On Tocogynecology, Botucatu Medical School (FMB), São Paulo State University (UNESP)Department of Physiotherapy and Occupational Therapy, School of Philosophy and Sciences (FCC), São Paulo State University (UNESP)Department of Physiotherapy and Occupational Therapy, School of Philosophy and Sciences (FCC), São Paulo State University (UNESP)Postgraduate Program On Tocogynecology, Botucatu Medical School (FMB), São Paulo State University (UNESP)Postgraduate Program On Tocogynecology, Botucatu Medical School (FMB), São Paulo State University (UNESP)Postgraduate Program On Tocogynecology, Botucatu Medical School (FMB), São Paulo State University (UNESP)Postgraduate Program On Tocogynecology, Botucatu Medical School (FMB), São Paulo State University (UNESP)Abstract Background Rectus abdominis muscle (RAM) myopathy is the underlying mechanism of pregnancy-specific urinary incontinence (PSUI), which has been shown to be a predictor of long-term urinary incontinence (UI). However, there is limited knowledge about selective ex vivo myo-mechanical assessment (MMA) of the RAM in healthy pregnant women, particularly those with PSUI. Results Our analysis revealed no specific quantitative parameters from the window analysis or contractile response of the RAM in ex vivo MMA. A gradual and consistent decline was predominantly observed in the peak and strength parameters for both groups. The qualitative contractile response analysis of the RAM using peak and strength parameters revealed three distinct behaviors under electrical stimulation: a progressive decrease in strength, sudden muscular arrest, and asynchrony with erratic fluctuations. In-depth quantitative analysis of the RAM ex vivo did not yield substantial differences between the two groups. However, qualitative analysis proved valuable in distinguishing the groups, revealing a tendency toward the loss of the progressive peak declines observed in the control group. Conclusion This study highlights the complexity of RAM myopathy in the context of PSUI and its potential impact on UI outcomes. Method In this cross-sectional study nested within a cohort, we examined ex vivo myography assessments in 87 mothers, comprising 48 with PSUI and 39 without PSUI, all of whom exhibited normal glucose tolerance. General data were extracted from our database, whereas RAM samples were collected during C-section for qualitative and quantitative ex vivo myography analysis, with a focus on initial and final baseline measurements, peak responses, strength, and duration times.https://doi.org/10.1186/s12938-025-01366-9Pregnancy-specific urinary incontinenceEx vivo myo-mechanical assayRectus abdominis muscleLong-term urinary incontinenceQualitative analysis |
| spellingShingle | Carolina N. F. Carvalho David R. A. Reyes Juliana F. Floriano Sofia B. C. V. Quiroz Rafael G. Oliveira Fernanda C. B. Alves Gabriela A. Garcia Luana F. Iamundo Adriely B. M. Magyori Caroline B. Prudencio Patricia S. Rossignoli Cristiane R. Pedroni Raghavendra L. S. Hallur Iracema M. P. Calderon Angélica M. P. Barbosa Marilza V. C. Rudge The Diamater Study Group Ex vivo myo-mechanical assessment of isolated rectus abdominis muscle in pregnancy-specific urinary incontinence: a cross-sectional study nested within the Diamater cohort BioMedical Engineering OnLine Pregnancy-specific urinary incontinence Ex vivo myo-mechanical assay Rectus abdominis muscle Long-term urinary incontinence Qualitative analysis |
| title | Ex vivo myo-mechanical assessment of isolated rectus abdominis muscle in pregnancy-specific urinary incontinence: a cross-sectional study nested within the Diamater cohort |
| title_full | Ex vivo myo-mechanical assessment of isolated rectus abdominis muscle in pregnancy-specific urinary incontinence: a cross-sectional study nested within the Diamater cohort |
| title_fullStr | Ex vivo myo-mechanical assessment of isolated rectus abdominis muscle in pregnancy-specific urinary incontinence: a cross-sectional study nested within the Diamater cohort |
| title_full_unstemmed | Ex vivo myo-mechanical assessment of isolated rectus abdominis muscle in pregnancy-specific urinary incontinence: a cross-sectional study nested within the Diamater cohort |
| title_short | Ex vivo myo-mechanical assessment of isolated rectus abdominis muscle in pregnancy-specific urinary incontinence: a cross-sectional study nested within the Diamater cohort |
| title_sort | ex vivo myo mechanical assessment of isolated rectus abdominis muscle in pregnancy specific urinary incontinence a cross sectional study nested within the diamater cohort |
| topic | Pregnancy-specific urinary incontinence Ex vivo myo-mechanical assay Rectus abdominis muscle Long-term urinary incontinence Qualitative analysis |
| url | https://doi.org/10.1186/s12938-025-01366-9 |
| work_keys_str_mv | AT carolinanfcarvalho exvivomyomechanicalassessmentofisolatedrectusabdominismuscleinpregnancyspecificurinaryincontinenceacrosssectionalstudynestedwithinthediamatercohort AT davidrareyes exvivomyomechanicalassessmentofisolatedrectusabdominismuscleinpregnancyspecificurinaryincontinenceacrosssectionalstudynestedwithinthediamatercohort AT julianaffloriano exvivomyomechanicalassessmentofisolatedrectusabdominismuscleinpregnancyspecificurinaryincontinenceacrosssectionalstudynestedwithinthediamatercohort AT sofiabcvquiroz exvivomyomechanicalassessmentofisolatedrectusabdominismuscleinpregnancyspecificurinaryincontinenceacrosssectionalstudynestedwithinthediamatercohort AT rafaelgoliveira exvivomyomechanicalassessmentofisolatedrectusabdominismuscleinpregnancyspecificurinaryincontinenceacrosssectionalstudynestedwithinthediamatercohort AT fernandacbalves exvivomyomechanicalassessmentofisolatedrectusabdominismuscleinpregnancyspecificurinaryincontinenceacrosssectionalstudynestedwithinthediamatercohort AT gabrielaagarcia exvivomyomechanicalassessmentofisolatedrectusabdominismuscleinpregnancyspecificurinaryincontinenceacrosssectionalstudynestedwithinthediamatercohort AT luanafiamundo exvivomyomechanicalassessmentofisolatedrectusabdominismuscleinpregnancyspecificurinaryincontinenceacrosssectionalstudynestedwithinthediamatercohort AT adrielybmmagyori exvivomyomechanicalassessmentofisolatedrectusabdominismuscleinpregnancyspecificurinaryincontinenceacrosssectionalstudynestedwithinthediamatercohort AT carolinebprudencio exvivomyomechanicalassessmentofisolatedrectusabdominismuscleinpregnancyspecificurinaryincontinenceacrosssectionalstudynestedwithinthediamatercohort AT patriciasrossignoli exvivomyomechanicalassessmentofisolatedrectusabdominismuscleinpregnancyspecificurinaryincontinenceacrosssectionalstudynestedwithinthediamatercohort AT cristianerpedroni exvivomyomechanicalassessmentofisolatedrectusabdominismuscleinpregnancyspecificurinaryincontinenceacrosssectionalstudynestedwithinthediamatercohort AT raghavendralshallur exvivomyomechanicalassessmentofisolatedrectusabdominismuscleinpregnancyspecificurinaryincontinenceacrosssectionalstudynestedwithinthediamatercohort AT iracemampcalderon exvivomyomechanicalassessmentofisolatedrectusabdominismuscleinpregnancyspecificurinaryincontinenceacrosssectionalstudynestedwithinthediamatercohort AT angelicampbarbosa exvivomyomechanicalassessmentofisolatedrectusabdominismuscleinpregnancyspecificurinaryincontinenceacrosssectionalstudynestedwithinthediamatercohort AT marilzavcrudge exvivomyomechanicalassessmentofisolatedrectusabdominismuscleinpregnancyspecificurinaryincontinenceacrosssectionalstudynestedwithinthediamatercohort AT thediamaterstudygroup exvivomyomechanicalassessmentofisolatedrectusabdominismuscleinpregnancyspecificurinaryincontinenceacrosssectionalstudynestedwithinthediamatercohort |