The Dynamic Interactions of m6A Modification and R-Loops: Implications for Genome Stability

R-loops, three-stranded RNA-DNA hybrid nucleic acid structures, are recognized for their roles in both physiological and pathological processes. Regulation of R-loops is critical for genome stability as disruption of R-loop homeostasis can lead to aberrant gene expression, replication stress, and DN...

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Main Authors: Nicholas Kim, Hong Sun
Format: Article
Language:English
Published: MDPI AG 2025-06-01
Series:Epigenomes
Subjects:
Online Access:https://www.mdpi.com/2075-4655/9/2/21
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author Nicholas Kim
Hong Sun
author_facet Nicholas Kim
Hong Sun
author_sort Nicholas Kim
collection DOAJ
description R-loops, three-stranded RNA-DNA hybrid nucleic acid structures, are recognized for their roles in both physiological and pathological processes. Regulation of R-loops is critical for genome stability as disruption of R-loop homeostasis can lead to aberrant gene expression, replication stress, and DNA damage. Recent studies suggest that the RNA modification, N6-methyladenosine (m6A), can modify R-loops and the writers, erasers, and readers of m6A are involved in the dynamic regulation of R-loops. Here, we discuss the reported functions of various m6A regulatory proteins in relation to R-loops, highlighting their distinct roles in recognizing and modulating the formation, stability, and resolution of these structures. We further examine the functional implications of m6A and R-loop interaction in human diseases, with a particular emphasis on their roles in cancer.
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spelling doaj-art-bc32f186bf7240edaef6733f9b54d5d72025-08-20T03:27:32ZengMDPI AGEpigenomes2075-46552025-06-01922110.3390/epigenomes9020021The Dynamic Interactions of m6A Modification and R-Loops: Implications for Genome StabilityNicholas Kim0Hong Sun1Division of Environmental Medicine, Department of Medicine, New York University Grossman School of Medicine, New York, NY 10010, USADivision of Environmental Medicine, Department of Medicine, New York University Grossman School of Medicine, New York, NY 10010, USAR-loops, three-stranded RNA-DNA hybrid nucleic acid structures, are recognized for their roles in both physiological and pathological processes. Regulation of R-loops is critical for genome stability as disruption of R-loop homeostasis can lead to aberrant gene expression, replication stress, and DNA damage. Recent studies suggest that the RNA modification, N6-methyladenosine (m6A), can modify R-loops and the writers, erasers, and readers of m6A are involved in the dynamic regulation of R-loops. Here, we discuss the reported functions of various m6A regulatory proteins in relation to R-loops, highlighting their distinct roles in recognizing and modulating the formation, stability, and resolution of these structures. We further examine the functional implications of m6A and R-loop interaction in human diseases, with a particular emphasis on their roles in cancer.https://www.mdpi.com/2075-4655/9/2/21R-loopsm6A RNA modificationgenome stabilityDNA damage
spellingShingle Nicholas Kim
Hong Sun
The Dynamic Interactions of m6A Modification and R-Loops: Implications for Genome Stability
Epigenomes
R-loops
m6A RNA modification
genome stability
DNA damage
title The Dynamic Interactions of m6A Modification and R-Loops: Implications for Genome Stability
title_full The Dynamic Interactions of m6A Modification and R-Loops: Implications for Genome Stability
title_fullStr The Dynamic Interactions of m6A Modification and R-Loops: Implications for Genome Stability
title_full_unstemmed The Dynamic Interactions of m6A Modification and R-Loops: Implications for Genome Stability
title_short The Dynamic Interactions of m6A Modification and R-Loops: Implications for Genome Stability
title_sort dynamic interactions of m6a modification and r loops implications for genome stability
topic R-loops
m6A RNA modification
genome stability
DNA damage
url https://www.mdpi.com/2075-4655/9/2/21
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