Fabrication of chitosan/CuO/grape seed extract biocomposite exhibiting synergistic antioxidant and antibacterial activity
The overuse of antibiotics has resulted in antibiotic-resistant microorganisms, a worldwide health concern. This resistance interferes with treatment methods and requires new approaches. Antibacterial and antioxidant nanocomposites represent promising fields of research. These nanocomposites may enh...
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Elsevier
2025-06-01
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| Series: | Carbohydrate Polymer Technologies and Applications |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666893925001823 |
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| author | Samar Mohammed Abd Alelah Muthanna O. Hussein Atheer A. Khashan |
| author_facet | Samar Mohammed Abd Alelah Muthanna O. Hussein Atheer A. Khashan |
| author_sort | Samar Mohammed Abd Alelah |
| collection | DOAJ |
| description | The overuse of antibiotics has resulted in antibiotic-resistant microorganisms, a worldwide health concern. This resistance interferes with treatment methods and requires new approaches. Antibacterial and antioxidant nanocomposites represent promising fields of research. These nanocomposites may enhance traditional antibiotics by mitigating cellular oxidative stress and combating pathogens. The unique characteristics of nanomaterials will be utilized to provide efficient treatments for resistant bacterial strains. The current study's goal was to investigate the antibacterial and antioxidant qualities of chitosan (CS)/copper oxide nanoparticles (CuONPs) mediated by grape seed extract (GSE). Consequently, the biosynthesized materials were characterized by using several techniques involving transmission electron microscopy (TEM), field emission scanning electron microscopy (FESEM), elemental analysis (EDX), and Fourier transform infrared spectrum (FTIR). The prepared CS/CuO/GSE nanocomposite revealed significant scavenging efficacy (74.2 ± 3.43 % at a 100 μg/mL) concentration for a DPPH radical (2,2-diphenyl-1-picrylhydrazyl), comparable with that of ascorbic acid (87.43 ± 0.89 %), regarded as a standard employing the agar well diffusion technique. The antibacterial efficacy of CS/GSE/CuO was examined, and minimal inhibitory concentrations were determined. In antibacterial assays, CS/CuO/GSE demonstrated the inhibition zone in Escherichia coli. at 9 mm and Streptococcus mutans at 14 mm at 100 µg/ml, respectively. Also, the MIC of the CS/CuO/GSE against Streptococcus mutans and Escherichia coli was determined to be 312μg/ml and 5 mg/ml, respectively. As demonstrated by experiments, the (CS/CuO/GSE) composite shows remarkable biological efficacy, particularly in antioxidant and antibacterial activity. |
| format | Article |
| id | doaj-art-bc309e83bbfd481f834e28642f7246fb |
| institution | Kabale University |
| issn | 2666-8939 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Carbohydrate Polymer Technologies and Applications |
| spelling | doaj-art-bc309e83bbfd481f834e28642f7246fb2025-08-20T03:45:10ZengElsevierCarbohydrate Polymer Technologies and Applications2666-89392025-06-011010084410.1016/j.carpta.2025.100844Fabrication of chitosan/CuO/grape seed extract biocomposite exhibiting synergistic antioxidant and antibacterial activitySamar Mohammed Abd Alelah0Muthanna O. Hussein1Atheer A. Khashan2Department of Chemistry, College of Science, University of Anbar, Ramadi, Iraq; Corresponding author.Department of Clinical Laboratory Sciences, College of Pharmacy, University of Anbar, Ramadi, IraqDepartment of Pharmacognosy, College of Pharmacy, University of Anbar, Ramadi, IraqThe overuse of antibiotics has resulted in antibiotic-resistant microorganisms, a worldwide health concern. This resistance interferes with treatment methods and requires new approaches. Antibacterial and antioxidant nanocomposites represent promising fields of research. These nanocomposites may enhance traditional antibiotics by mitigating cellular oxidative stress and combating pathogens. The unique characteristics of nanomaterials will be utilized to provide efficient treatments for resistant bacterial strains. The current study's goal was to investigate the antibacterial and antioxidant qualities of chitosan (CS)/copper oxide nanoparticles (CuONPs) mediated by grape seed extract (GSE). Consequently, the biosynthesized materials were characterized by using several techniques involving transmission electron microscopy (TEM), field emission scanning electron microscopy (FESEM), elemental analysis (EDX), and Fourier transform infrared spectrum (FTIR). The prepared CS/CuO/GSE nanocomposite revealed significant scavenging efficacy (74.2 ± 3.43 % at a 100 μg/mL) concentration for a DPPH radical (2,2-diphenyl-1-picrylhydrazyl), comparable with that of ascorbic acid (87.43 ± 0.89 %), regarded as a standard employing the agar well diffusion technique. The antibacterial efficacy of CS/GSE/CuO was examined, and minimal inhibitory concentrations were determined. In antibacterial assays, CS/CuO/GSE demonstrated the inhibition zone in Escherichia coli. at 9 mm and Streptococcus mutans at 14 mm at 100 µg/ml, respectively. Also, the MIC of the CS/CuO/GSE against Streptococcus mutans and Escherichia coli was determined to be 312μg/ml and 5 mg/ml, respectively. As demonstrated by experiments, the (CS/CuO/GSE) composite shows remarkable biological efficacy, particularly in antioxidant and antibacterial activity.http://www.sciencedirect.com/science/article/pii/S2666893925001823BionanocompositeChitosanCuO nanoparticlesDPPHGrape seed extractMIC |
| spellingShingle | Samar Mohammed Abd Alelah Muthanna O. Hussein Atheer A. Khashan Fabrication of chitosan/CuO/grape seed extract biocomposite exhibiting synergistic antioxidant and antibacterial activity Carbohydrate Polymer Technologies and Applications Bionanocomposite Chitosan CuO nanoparticles DPPH Grape seed extract MIC |
| title | Fabrication of chitosan/CuO/grape seed extract biocomposite exhibiting synergistic antioxidant and antibacterial activity |
| title_full | Fabrication of chitosan/CuO/grape seed extract biocomposite exhibiting synergistic antioxidant and antibacterial activity |
| title_fullStr | Fabrication of chitosan/CuO/grape seed extract biocomposite exhibiting synergistic antioxidant and antibacterial activity |
| title_full_unstemmed | Fabrication of chitosan/CuO/grape seed extract biocomposite exhibiting synergistic antioxidant and antibacterial activity |
| title_short | Fabrication of chitosan/CuO/grape seed extract biocomposite exhibiting synergistic antioxidant and antibacterial activity |
| title_sort | fabrication of chitosan cuo grape seed extract biocomposite exhibiting synergistic antioxidant and antibacterial activity |
| topic | Bionanocomposite Chitosan CuO nanoparticles DPPH Grape seed extract MIC |
| url | http://www.sciencedirect.com/science/article/pii/S2666893925001823 |
| work_keys_str_mv | AT samarmohammedabdalelah fabricationofchitosancuograpeseedextractbiocompositeexhibitingsynergisticantioxidantandantibacterialactivity AT muthannaohussein fabricationofchitosancuograpeseedextractbiocompositeexhibitingsynergisticantioxidantandantibacterialactivity AT atheerakhashan fabricationofchitosancuograpeseedextractbiocompositeexhibitingsynergisticantioxidantandantibacterialactivity |