New Viruses Infecting Hyperthermophilic Bacterium <em>Thermus thermophilus</em>
Highly diverse phages infecting thermophilic bacteria of the <i>Thermus</i> genus have been isolated over the years from hot springs around the world. Many of these phages are unique, rely on highly unusual developmental strategies, and encode novel enzymes. The variety of <i>Therm...
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2024-09-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/16/9/1410 |
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| author | Matvey Kolesnik Constantine Pavlov Alina Demkina Aleksei Samolygo Karyna Karneyeva Anna Trofimova Olga S. Sokolova Andrei V. Moiseenko Maria Kirsanova Konstantin Severinov |
| author_facet | Matvey Kolesnik Constantine Pavlov Alina Demkina Aleksei Samolygo Karyna Karneyeva Anna Trofimova Olga S. Sokolova Andrei V. Moiseenko Maria Kirsanova Konstantin Severinov |
| author_sort | Matvey Kolesnik |
| collection | DOAJ |
| description | Highly diverse phages infecting thermophilic bacteria of the <i>Thermus</i> genus have been isolated over the years from hot springs around the world. Many of these phages are unique, rely on highly unusual developmental strategies, and encode novel enzymes. The variety of <i>Thermus</i> phages is clearly undersampled, as evidenced, for example, by a paucity of phage-matching spacers in <i>Thermus</i> CRISPR arrays. Using water samples collected from hot springs in the Kunashir Island from the Kuril archipelago and from the Tsaishi and Nokalakevi districts in the Republic of Georgia, we isolated several distinct phages infecting laboratory strains of <i>Thermus thermophilus</i>. Genomic sequence analysis of 11 phages revealed both close relatives of previously described <i>Thermus</i> phages isolated from geographically distant sites, as well as phages with very limited similarity to earlier isolates. Comparative analysis allowed us to predict several accessory phage genes whose products may be involved in host defense/interviral warfare, including a putative Type V CRISPR-<i>cas</i> system. |
| format | Article |
| id | doaj-art-bc27464271b54148977a44626b8f112e |
| institution | OA Journals |
| issn | 1999-4915 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj-art-bc27464271b54148977a44626b8f112e2025-08-20T01:56:13ZengMDPI AGViruses1999-49152024-09-01169141010.3390/v16091410New Viruses Infecting Hyperthermophilic Bacterium <em>Thermus thermophilus</em>Matvey Kolesnik0Constantine Pavlov1Alina Demkina2Aleksei Samolygo3Karyna Karneyeva4Anna Trofimova5Olga S. Sokolova6Andrei V. Moiseenko7Maria Kirsanova8Konstantin Severinov9Institute of Gene Biology Russian Academy of Sciences, 119334 Moscow, RussiaInstitute of Gene Biology Russian Academy of Sciences, 119334 Moscow, RussiaInstitute of Gene Biology Russian Academy of Sciences, 119334 Moscow, RussiaInstitute of Gene Biology Russian Academy of Sciences, 119334 Moscow, RussiaInstitute of Gene Biology Russian Academy of Sciences, 119334 Moscow, RussiaInstitute of Gene Biology Russian Academy of Sciences, 119334 Moscow, RussiaFaculty of Biology, MSU-BIT University, Shenzhen 518172, ChinaFaculty of Biology, Lomonosov Moscow State University, 119234 Moscow, RussiaInstitute of Gene Biology Russian Academy of Sciences, 119334 Moscow, RussiaInstitute of Gene Biology Russian Academy of Sciences, 119334 Moscow, RussiaHighly diverse phages infecting thermophilic bacteria of the <i>Thermus</i> genus have been isolated over the years from hot springs around the world. Many of these phages are unique, rely on highly unusual developmental strategies, and encode novel enzymes. The variety of <i>Thermus</i> phages is clearly undersampled, as evidenced, for example, by a paucity of phage-matching spacers in <i>Thermus</i> CRISPR arrays. Using water samples collected from hot springs in the Kunashir Island from the Kuril archipelago and from the Tsaishi and Nokalakevi districts in the Republic of Georgia, we isolated several distinct phages infecting laboratory strains of <i>Thermus thermophilus</i>. Genomic sequence analysis of 11 phages revealed both close relatives of previously described <i>Thermus</i> phages isolated from geographically distant sites, as well as phages with very limited similarity to earlier isolates. Comparative analysis allowed us to predict several accessory phage genes whose products may be involved in host defense/interviral warfare, including a putative Type V CRISPR-<i>cas</i> system.https://www.mdpi.com/1999-4915/16/9/1410bacteriophageCRISPR-Cas systemmobile genetic elements |
| spellingShingle | Matvey Kolesnik Constantine Pavlov Alina Demkina Aleksei Samolygo Karyna Karneyeva Anna Trofimova Olga S. Sokolova Andrei V. Moiseenko Maria Kirsanova Konstantin Severinov New Viruses Infecting Hyperthermophilic Bacterium <em>Thermus thermophilus</em> Viruses bacteriophage CRISPR-Cas system mobile genetic elements |
| title | New Viruses Infecting Hyperthermophilic Bacterium <em>Thermus thermophilus</em> |
| title_full | New Viruses Infecting Hyperthermophilic Bacterium <em>Thermus thermophilus</em> |
| title_fullStr | New Viruses Infecting Hyperthermophilic Bacterium <em>Thermus thermophilus</em> |
| title_full_unstemmed | New Viruses Infecting Hyperthermophilic Bacterium <em>Thermus thermophilus</em> |
| title_short | New Viruses Infecting Hyperthermophilic Bacterium <em>Thermus thermophilus</em> |
| title_sort | new viruses infecting hyperthermophilic bacterium em thermus thermophilus em |
| topic | bacteriophage CRISPR-Cas system mobile genetic elements |
| url | https://www.mdpi.com/1999-4915/16/9/1410 |
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