Rapid and sensitive detection of Mycobacterium tuberculosis using the RPA/Cas12f1_ge4.1 system with fluorescence and lateral flow readouts

Rapid and sensitive detection of Mycobacterium tuberculosis using the RPA/Cas12f1_ge4.1 system with fluorescence and lateral flow readouts

ABSTRACT Tuberculosis remains a major global health threat, with existing detection methods often limited by efficiency and resource demands. Our previous PAM-dependent dsDNA Target-activated Cas12f1 Trans Reporter (PDTCTR) fluorescence sensing platform, while effective for PAM-dependent pathogen de...

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Bibliographic Details
Main Authors: Zhongliang Deng, Xingyong Weng, Honghua Tang, Tintao Zou, Xuan Zhou, Hangxi Liu, Piaoting Wen, Gemiao Luo, Tian Gan, Jun He
Format: Article
Language:English
Published: American Society for Microbiology 2025-07-01
Series:Microbiology Spectrum
Subjects:
UnCas12f1
recombinase polymerase amplification (RPA)
Mycobacterium tuberculosis
RPA/Cas12f1_ge4.1
fluorescence
lateral flow readouts
Online Access:https://journals.asm.org/doi/10.1128/spectrum.02652-24
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