Effect of moringa seed (Moringa oleifera Lam.) infusion on stamina performance in male mice

Background Individuals experience varying levels of fatigue and stamina. Moringa oleifera Lam. Seed extract relieves fatigue due to its high levels of antioxidants and glucosinolate glucomoringin, which reduces intracellular oxidative stress. Objectives This study aimed to determine the effectiv...

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Main Authors: Hanifati Husna, Andriyanto Andriyanto, Wasmen Manalu, Kusdiantoro Mohamad
Format: Article
Language:English
Published: School of Veterinary Medicine and Biomedical Sciences, IPB University 2025-07-01
Series:Current Biomedicine
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Online Access:https://journal.ipb.ac.id/index.php/currbiomed/article/view/64242
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author Hanifati Husna
Andriyanto Andriyanto
Wasmen Manalu
Kusdiantoro Mohamad
author_facet Hanifati Husna
Andriyanto Andriyanto
Wasmen Manalu
Kusdiantoro Mohamad
author_sort Hanifati Husna
collection DOAJ
description Background Individuals experience varying levels of fatigue and stamina. Moringa oleifera Lam. Seed extract relieves fatigue due to its high levels of antioxidants and glucosinolate glucomoringin, which reduces intracellular oxidative stress. Objectives This study aimed to determine the effectiveness of infusion of M. oleifera seed as a stamina stimulator in mice. Methods This experiment used 25 male mice divided into five groups (five mice in each group), consisting of M. oleifera seed infusion at doses of 0, 1, 3, and 5 g/kg BW as the negative control and treatment groups, and caffeine at a dose of 6.5 mg/kg BW as the positive control group. Stamina effectiveness was evaluated using a swimming endurance test that measured swimming duration and physiological parameters every 15 min for 2 h after administration. Data were analyzed using analysis of variance (ANOVA) followed by Tukey’s test. Results M. oleifera seed infusion at a dose of 3 g/kg BW had the shortest swimming duration difference (Δ) value, with no difference compared to the 5 g/kg BW and caffeine groups, but was significantly different (P<0.05) compared to the 0 and 1 g/kg BW groups. M. oleifera seed infusion at a dose of 3 g/kg BW also showed a lower delta value for body surface temperature and heartbeat rate compared to the negative control, and a lower delta value for the respiratory rate compared to the 5 g/kg BW group. Conclusion M. oleifera seed infusion potentially maintains stamina performance in mice at a dose of 3 g/kg BW.
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publisher School of Veterinary Medicine and Biomedical Sciences, IPB University
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spelling doaj-art-bc1f6de829f540aba522b084c287e8082025-08-20T02:43:12ZengSchool of Veterinary Medicine and Biomedical Sciences, IPB UniversityCurrent Biomedicine2962-84902025-07-013210.29244/currbiomed.3.2.67Effect of moringa seed (Moringa oleifera Lam.) infusion on stamina performance in male mice Hanifati Husna0 Andriyanto Andriyanto1 Wasmen Manalu2 Kusdiantoro Mohamad3Study Program of Veterinary Medicine, School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, IndonesiaDivision of Veterinary Pharmacology and Toxicology, School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, IndonesiaDivision of Physiology, School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, IndonesiaDivision of Anatomy, Histology, and Embryology, School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia Background Individuals experience varying levels of fatigue and stamina. Moringa oleifera Lam. Seed extract relieves fatigue due to its high levels of antioxidants and glucosinolate glucomoringin, which reduces intracellular oxidative stress. Objectives This study aimed to determine the effectiveness of infusion of M. oleifera seed as a stamina stimulator in mice. Methods This experiment used 25 male mice divided into five groups (five mice in each group), consisting of M. oleifera seed infusion at doses of 0, 1, 3, and 5 g/kg BW as the negative control and treatment groups, and caffeine at a dose of 6.5 mg/kg BW as the positive control group. Stamina effectiveness was evaluated using a swimming endurance test that measured swimming duration and physiological parameters every 15 min for 2 h after administration. Data were analyzed using analysis of variance (ANOVA) followed by Tukey’s test. Results M. oleifera seed infusion at a dose of 3 g/kg BW had the shortest swimming duration difference (Δ) value, with no difference compared to the 5 g/kg BW and caffeine groups, but was significantly different (P<0.05) compared to the 0 and 1 g/kg BW groups. M. oleifera seed infusion at a dose of 3 g/kg BW also showed a lower delta value for body surface temperature and heartbeat rate compared to the negative control, and a lower delta value for the respiratory rate compared to the 5 g/kg BW group. Conclusion M. oleifera seed infusion potentially maintains stamina performance in mice at a dose of 3 g/kg BW. https://journal.ipb.ac.id/index.php/currbiomed/article/view/64242anti-fatigueinfusionMoringa oleiferastamina performancemice
spellingShingle Hanifati Husna
Andriyanto Andriyanto
Wasmen Manalu
Kusdiantoro Mohamad
Effect of moringa seed (Moringa oleifera Lam.) infusion on stamina performance in male mice
Current Biomedicine
anti-fatigue
infusion
Moringa oleifera
stamina performance
mice
title Effect of moringa seed (Moringa oleifera Lam.) infusion on stamina performance in male mice
title_full Effect of moringa seed (Moringa oleifera Lam.) infusion on stamina performance in male mice
title_fullStr Effect of moringa seed (Moringa oleifera Lam.) infusion on stamina performance in male mice
title_full_unstemmed Effect of moringa seed (Moringa oleifera Lam.) infusion on stamina performance in male mice
title_short Effect of moringa seed (Moringa oleifera Lam.) infusion on stamina performance in male mice
title_sort effect of moringa seed moringa oleifera lam infusion on stamina performance in male mice
topic anti-fatigue
infusion
Moringa oleifera
stamina performance
mice
url https://journal.ipb.ac.id/index.php/currbiomed/article/view/64242
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AT andriyantoandriyanto effectofmoringaseedmoringaoleiferalaminfusiononstaminaperformanceinmalemice
AT wasmenmanalu effectofmoringaseedmoringaoleiferalaminfusiononstaminaperformanceinmalemice
AT kusdiantoromohamad effectofmoringaseedmoringaoleiferalaminfusiononstaminaperformanceinmalemice