Understanding Investigational Perspective of Antioxidant and Antibacterial Properties of Rice

Whole-grain rice has gained significant attention for its potential health benefits, particularly because of its antioxidant and antibacterial properties. Despite this growing interest, there remains a pressing need for a comprehensive evaluation of the methods used to analyze these bioactive compou...

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Main Authors: Intan Farahanah, Shariza Sahudin, Hannis Fadzillah Mohsin, Siti Alwani Ariffin, Liyana Dhamirah Aminuddin
Format: Article
Language:English
Published: Elsevier 2025-01-01
Series:Rice Science
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Online Access:http://www.sciencedirect.com/science/article/pii/S1672630824000908
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author Intan Farahanah
Shariza Sahudin
Hannis Fadzillah Mohsin
Siti Alwani Ariffin
Liyana Dhamirah Aminuddin
author_facet Intan Farahanah
Shariza Sahudin
Hannis Fadzillah Mohsin
Siti Alwani Ariffin
Liyana Dhamirah Aminuddin
author_sort Intan Farahanah
collection DOAJ
description Whole-grain rice has gained significant attention for its potential health benefits, particularly because of its antioxidant and antibacterial properties. Despite this growing interest, there remains a pressing need for a comprehensive evaluation of the methods used to analyze these bioactive compounds, considering the diversity of rice types and the influence of environmental factors. This review aims to provide an in-depth overview of recent analytical methods, particularly those employed over the last five years, to assess the antioxidant and antibacterial properties of rice. It focuses on commonly used antioxidant assays, such as the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity assay, which encompasses both single electron transfer and hydrogen atom transfer mechanisms. Other antioxidant assays are also discussed, alongside methods used to evaluate antibacterial properties, including disc diffusion, well diffusion, broth or agar dilution, and advanced techniques such as flow cytofluorometric and bioluminescent assays. The review further explores the bacterial strains most frequently studied, including Bacillus cereus, Listeria monocytogenes, Escherichia coli, and Salmonella species. It highlights the key techniques and parameters employed to ascertain and quantify the antioxidant and antibacterial potential of rice, while critically analysing the strengths and limitations of these approaches. By synthesizing current methods and offering insights into their application, this review can be served as a guide for future research aimed at standardizing and improving the assessment of rice’s bioactive properties, paving the way for broader scientific and practical applications.
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spelling doaj-art-bc1ec9808acf4d2e8bbc12d7b299f1c02025-02-12T05:30:49ZengElsevierRice Science1672-63082025-01-013211531Understanding Investigational Perspective of Antioxidant and Antibacterial Properties of RiceIntan Farahanah0Shariza Sahudin1Hannis Fadzillah Mohsin2Siti Alwani Ariffin3Liyana Dhamirah Aminuddin4Department of Pharmaceutical Technology, Faculty of Pharmacy, Universiti Teknologi Mara, Puncak Alam Campus, Selangor 42300, MalaysiaDepartment of Pharmaceutical Technology, Faculty of Pharmacy, Universiti Teknologi Mara, Puncak Alam Campus, Selangor 42300, Malaysia; Corresponding author.Department of Chemistry, Faculty of Pharmacy, Universiti Teknologi Mara, Puncak Alam Campus, Selangor 42300, MalaysiaDepartment of Pharmacology and Life Sciences, Faculty of Pharmacy, Universiti Teknologi Mara, Puncak Alam Campus, Selangor 42300, MalaysiaFaculty of Medicine, Universiti Teknologi Mara, Sungai Buloh Campus, Selangor 42300, MalaysiaWhole-grain rice has gained significant attention for its potential health benefits, particularly because of its antioxidant and antibacterial properties. Despite this growing interest, there remains a pressing need for a comprehensive evaluation of the methods used to analyze these bioactive compounds, considering the diversity of rice types and the influence of environmental factors. This review aims to provide an in-depth overview of recent analytical methods, particularly those employed over the last five years, to assess the antioxidant and antibacterial properties of rice. It focuses on commonly used antioxidant assays, such as the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity assay, which encompasses both single electron transfer and hydrogen atom transfer mechanisms. Other antioxidant assays are also discussed, alongside methods used to evaluate antibacterial properties, including disc diffusion, well diffusion, broth or agar dilution, and advanced techniques such as flow cytofluorometric and bioluminescent assays. The review further explores the bacterial strains most frequently studied, including Bacillus cereus, Listeria monocytogenes, Escherichia coli, and Salmonella species. It highlights the key techniques and parameters employed to ascertain and quantify the antioxidant and antibacterial potential of rice, while critically analysing the strengths and limitations of these approaches. By synthesizing current methods and offering insights into their application, this review can be served as a guide for future research aimed at standardizing and improving the assessment of rice’s bioactive properties, paving the way for broader scientific and practical applications.http://www.sciencedirect.com/science/article/pii/S1672630824000908pigmented riceantioxidant activityantibacterial activity
spellingShingle Intan Farahanah
Shariza Sahudin
Hannis Fadzillah Mohsin
Siti Alwani Ariffin
Liyana Dhamirah Aminuddin
Understanding Investigational Perspective of Antioxidant and Antibacterial Properties of Rice
Rice Science
pigmented rice
antioxidant activity
antibacterial activity
title Understanding Investigational Perspective of Antioxidant and Antibacterial Properties of Rice
title_full Understanding Investigational Perspective of Antioxidant and Antibacterial Properties of Rice
title_fullStr Understanding Investigational Perspective of Antioxidant and Antibacterial Properties of Rice
title_full_unstemmed Understanding Investigational Perspective of Antioxidant and Antibacterial Properties of Rice
title_short Understanding Investigational Perspective of Antioxidant and Antibacterial Properties of Rice
title_sort understanding investigational perspective of antioxidant and antibacterial properties of rice
topic pigmented rice
antioxidant activity
antibacterial activity
url http://www.sciencedirect.com/science/article/pii/S1672630824000908
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