Preparation and biological performance of a stepwise degradable hyaluronic acid hydrogel hemostatic sponge loaded with steroids
Chronic rhinosinusitis (CRS) is associated with postoperative complications such as adhesions and recurrent inflammation. Nasal implants are clinically utilized as adjuncts to endoscopic sinus surgery (ESS) for controlling hemorrhage and enhancing drug delivery. However, existing implants, such as N...
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Elsevier
2025-06-01
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| Series: | Carbohydrate Polymer Technologies and Applications |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666893925001501 |
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| author | Bohuai Xu Wei Wang Jiayan Wang Jibin Huang Di Wu Ming Xu |
| author_facet | Bohuai Xu Wei Wang Jiayan Wang Jibin Huang Di Wu Ming Xu |
| author_sort | Bohuai Xu |
| collection | DOAJ |
| description | Chronic rhinosinusitis (CRS) is associated with postoperative complications such as adhesions and recurrent inflammation. Nasal implants are clinically utilized as adjuncts to endoscopic sinus surgery (ESS) for controlling hemorrhage and enhancing drug delivery. However, existing implants, such as NASOPORE®, exhibit uniform degradation patterns. In this study, we developed a stepwise degradable hyaluronic acid (HA) hydrogel hemostatic sponge loaded with mometasone furoate (Elocon Cream), designed to synchronize its degradation with the phases of wound healing. The sponge demonstrated a dual-phase degradation profile: rapid hemostatic material clearance (65 % mass loss by Day 5) followed by sustained HA hydrogel degradation (12 % residual mass at Day 14), enabling prolonged mucosal MF retention.The HA@F127-MF sponge exhibited exceptional water absorption (swelling ratio: 1470 ± 141 %), mechanical stability (compressive modulus: 12.8 ± 1.8 kPa), and controlled drug release (85.44 ± 3.22 % cumulative release over 15 days). In vivo studies revealed significant anti-inflammatory effects, with TNF-α, IL-5, and periostin levels reduced by 62 %, 68 %, and 54 %, respectively (p < 0.01 vs. control), attributed to HA’s CD44-mediated anti-inflammatory activity (100 kDa) combined with MF’s corticosteroid action. Histological analysis confirmed minimal inflammation and no systemic toxicity.This study addresses critical limitations of current implants by introducing a stepwise degradable design that enhances hemostasis and sustained drug delivery, offering a promising strategy for CRS postoperative management. |
| format | Article |
| id | doaj-art-bc1281832e694a02b336167a73b2b115 |
| institution | DOAJ |
| issn | 2666-8939 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Carbohydrate Polymer Technologies and Applications |
| spelling | doaj-art-bc1281832e694a02b336167a73b2b1152025-08-20T03:20:58ZengElsevierCarbohydrate Polymer Technologies and Applications2666-89392025-06-011010081210.1016/j.carpta.2025.100812Preparation and biological performance of a stepwise degradable hyaluronic acid hydrogel hemostatic sponge loaded with steroidsBohuai Xu0Wei Wang1Jiayan Wang2Jibin Huang3Di Wu4Ming Xu5Department of otolaryngology head and neck surgery, The First Affiliated Hospital of Ningbo University, Ningbo, Zhejiang 315000, PR ChinaDepartment of Otorhinolaryngology, Ningbo Municipal Hospital of Traditional Chinese Medicine, Affiliated Hospital of Zhejiang Chinese Medical University, Ningbo 315012, PR ChinaDepartment of Otorhinolaryngology, Ningbo Municipal Hospital of Traditional Chinese Medicine, Affiliated Hospital of Zhejiang Chinese Medical University, Ningbo 315012, PR ChinaDepartment of Otorhinolaryngology, Ningbo Municipal Hospital of Traditional Chinese Medicine, Affiliated Hospital of Zhejiang Chinese Medical University, Ningbo 315012, PR ChinaDepartment of Otorhinolaryngology, Ningbo Municipal Hospital of Traditional Chinese Medicine, Affiliated Hospital of Zhejiang Chinese Medical University, Ningbo 315012, PR ChinaDepartment of Otorhinolaryngology, Ningbo Municipal Hospital of Traditional Chinese Medicine, Affiliated Hospital of Zhejiang Chinese Medical University, Ningbo 315012, PR China; Corresponding author at: Department of Otorhinolaryngology, Ningbo Municipal Hospital of Traditional Chinese Medicine (TCM), Affiliated Hospital of Zhejiang Chinese Medical University, No 819 Liyuan Roa, Haishu District, Ningbo 315012, China.Chronic rhinosinusitis (CRS) is associated with postoperative complications such as adhesions and recurrent inflammation. Nasal implants are clinically utilized as adjuncts to endoscopic sinus surgery (ESS) for controlling hemorrhage and enhancing drug delivery. However, existing implants, such as NASOPORE®, exhibit uniform degradation patterns. In this study, we developed a stepwise degradable hyaluronic acid (HA) hydrogel hemostatic sponge loaded with mometasone furoate (Elocon Cream), designed to synchronize its degradation with the phases of wound healing. The sponge demonstrated a dual-phase degradation profile: rapid hemostatic material clearance (65 % mass loss by Day 5) followed by sustained HA hydrogel degradation (12 % residual mass at Day 14), enabling prolonged mucosal MF retention.The HA@F127-MF sponge exhibited exceptional water absorption (swelling ratio: 1470 ± 141 %), mechanical stability (compressive modulus: 12.8 ± 1.8 kPa), and controlled drug release (85.44 ± 3.22 % cumulative release over 15 days). In vivo studies revealed significant anti-inflammatory effects, with TNF-α, IL-5, and periostin levels reduced by 62 %, 68 %, and 54 %, respectively (p < 0.01 vs. control), attributed to HA’s CD44-mediated anti-inflammatory activity (100 kDa) combined with MF’s corticosteroid action. Histological analysis confirmed minimal inflammation and no systemic toxicity.This study addresses critical limitations of current implants by introducing a stepwise degradable design that enhances hemostasis and sustained drug delivery, offering a promising strategy for CRS postoperative management.http://www.sciencedirect.com/science/article/pii/S2666893925001501Chronic rhinosinusitisPostoperative treatmentNasal implantsStepwise degradable hydrogelHyaluronic acidSteroids |
| spellingShingle | Bohuai Xu Wei Wang Jiayan Wang Jibin Huang Di Wu Ming Xu Preparation and biological performance of a stepwise degradable hyaluronic acid hydrogel hemostatic sponge loaded with steroids Carbohydrate Polymer Technologies and Applications Chronic rhinosinusitis Postoperative treatment Nasal implants Stepwise degradable hydrogel Hyaluronic acid Steroids |
| title | Preparation and biological performance of a stepwise degradable hyaluronic acid hydrogel hemostatic sponge loaded with steroids |
| title_full | Preparation and biological performance of a stepwise degradable hyaluronic acid hydrogel hemostatic sponge loaded with steroids |
| title_fullStr | Preparation and biological performance of a stepwise degradable hyaluronic acid hydrogel hemostatic sponge loaded with steroids |
| title_full_unstemmed | Preparation and biological performance of a stepwise degradable hyaluronic acid hydrogel hemostatic sponge loaded with steroids |
| title_short | Preparation and biological performance of a stepwise degradable hyaluronic acid hydrogel hemostatic sponge loaded with steroids |
| title_sort | preparation and biological performance of a stepwise degradable hyaluronic acid hydrogel hemostatic sponge loaded with steroids |
| topic | Chronic rhinosinusitis Postoperative treatment Nasal implants Stepwise degradable hydrogel Hyaluronic acid Steroids |
| url | http://www.sciencedirect.com/science/article/pii/S2666893925001501 |
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