Production and application of monoclonal antibodies to GST
Two hybridoma cell lines (4B3 and 1H10) secreting monoclonal antibodies (McAbs) against recombinant GST were produced by fusing mouse myeloma cells (SP2/0) with spleen cells from BALB/C immunized by the purified GST protein and subsequently three-time limited dilution method. The titres of ascitic f...
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| Format: | Article |
| Language: | English |
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Zhejiang University Press
2006-07-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2006.04.0383 |
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| _version_ | 1849413385141092352 |
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| author | WU Jian-xiang CHEN Tian-jia ZHOU Xue-ping |
| author_facet | WU Jian-xiang CHEN Tian-jia ZHOU Xue-ping |
| author_sort | WU Jian-xiang |
| collection | DOAJ |
| description | Two hybridoma cell lines (4B3 and 1H10) secreting monoclonal antibodies (McAbs) against recombinant GST were produced by fusing mouse myeloma cells (SP2/0) with spleen cells from BALB/C immunized by the purified GST protein and subsequently three-time limited dilution method. The titres of ascitic fluids of the two McAbs were up to 10<sup>-6</sup> in indirect ELISA. Isotypes and subclasses of 1H10 and 4B3 all belong to IgG1, κ. Western-blot showed that the two McAbs can specifically react with GST-IBV-S1-F and recombinant GST. The McAb 1H10 has successfully been used in immunoaffinity chromatography of GST fusion protein. |
| format | Article |
| id | doaj-art-bc10e63dc8bd41569eb88f16221ebcb4 |
| institution | Kabale University |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2006-07-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-bc10e63dc8bd41569eb88f16221ebcb42025-08-20T03:34:08ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552006-07-013238338610.3785/1008-9209.2006.04.038310089209Production and application of monoclonal antibodies to GSTWU Jian-xiangCHEN Tian-jiaZHOU Xue-pingTwo hybridoma cell lines (4B3 and 1H10) secreting monoclonal antibodies (McAbs) against recombinant GST were produced by fusing mouse myeloma cells (SP2/0) with spleen cells from BALB/C immunized by the purified GST protein and subsequently three-time limited dilution method. The titres of ascitic fluids of the two McAbs were up to 10<sup>-6</sup> in indirect ELISA. Isotypes and subclasses of 1H10 and 4B3 all belong to IgG1, κ. Western-blot showed that the two McAbs can specifically react with GST-IBV-S1-F and recombinant GST. The McAb 1H10 has successfully been used in immunoaffinity chromatography of GST fusion protein.https://www.academax.com/doi/10.3785/1008-9209.2006.04.0383GSThybridoma cellmonoclonal antibodies |
| spellingShingle | WU Jian-xiang CHEN Tian-jia ZHOU Xue-ping Production and application of monoclonal antibodies to GST 浙江大学学报. 农业与生命科学版 GST hybridoma cell monoclonal antibodies |
| title | Production and application of monoclonal antibodies to GST |
| title_full | Production and application of monoclonal antibodies to GST |
| title_fullStr | Production and application of monoclonal antibodies to GST |
| title_full_unstemmed | Production and application of monoclonal antibodies to GST |
| title_short | Production and application of monoclonal antibodies to GST |
| title_sort | production and application of monoclonal antibodies to gst |
| topic | GST hybridoma cell monoclonal antibodies |
| url | https://www.academax.com/doi/10.3785/1008-9209.2006.04.0383 |
| work_keys_str_mv | AT wujianxiang productionandapplicationofmonoclonalantibodiestogst AT chentianjia productionandapplicationofmonoclonalantibodiestogst AT zhouxueping productionandapplicationofmonoclonalantibodiestogst |