Epsins Regulate Mouse Embryonic Stem Cell Exit from Pluripotency and Neural Commitment by Controlling Notch Activation
Epsins are part of the internalization machinery pivotal to control clathrin-mediated endocytosis. Here, we report that epsin family members are expressed in mouse embryonic stem cells (mESCs) and that epsin1/2 knockdown alters both mESC exits from pluripotency and their differentiation. Furthermore...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2019/4084351 |
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| author | Marina Cardano Jacopo Zasso Luca Ruggiero Giuseppina Di Giacomo Matteo Marcatili Ottavio Cremona Luciano Conti |
| author_facet | Marina Cardano Jacopo Zasso Luca Ruggiero Giuseppina Di Giacomo Matteo Marcatili Ottavio Cremona Luciano Conti |
| author_sort | Marina Cardano |
| collection | DOAJ |
| description | Epsins are part of the internalization machinery pivotal to control clathrin-mediated endocytosis. Here, we report that epsin family members are expressed in mouse embryonic stem cells (mESCs) and that epsin1/2 knockdown alters both mESC exits from pluripotency and their differentiation. Furthermore, we show that epsin1/2 knockdown compromises the correct polarization and division of mESC-derived neural progenitors and their conversion into expandable radial glia-like neural stem cells. Finally, we provide evidence that Notch signaling is impaired following epsin1/2 knockdown and that experimental restoration of Notch signaling rescues the epsin-mediated phenotypes. We conclude that epsins contribute to control mESC exit from pluripotency and allow their neural differentiation by appropriate modulation of Notch signaling. |
| format | Article |
| id | doaj-art-bc1037637f26433b879ca7cf2cfec26d |
| institution | OA Journals |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-bc1037637f26433b879ca7cf2cfec26d2025-08-20T02:18:43ZengWileyStem Cells International1687-966X1687-96782019-01-01201910.1155/2019/40843514084351Epsins Regulate Mouse Embryonic Stem Cell Exit from Pluripotency and Neural Commitment by Controlling Notch ActivationMarina Cardano0Jacopo Zasso1Luca Ruggiero2Giuseppina Di Giacomo3Matteo Marcatili4Ottavio Cremona5Luciano Conti6Laboratory of Stem Cell Biology, Department of Cellular, Computational and Integrative Biology (CIBIO), Università degli Studi di Trento, Trento, ItalyLaboratory of Stem Cell Biology, Department of Cellular, Computational and Integrative Biology (CIBIO), Università degli Studi di Trento, Trento, ItalyUniversità Vita Salute San Raffaele, Ospedale San Raffaele, Division of Neuroscience, Milan, ItalyUniversità Vita Salute San Raffaele, Ospedale San Raffaele, Division of Neuroscience, Milan, ItalyLaboratory of Stem Cell Biology, Department of Cellular, Computational and Integrative Biology (CIBIO), Università degli Studi di Trento, Trento, ItalyUniversità Vita Salute San Raffaele, Ospedale San Raffaele, Division of Neuroscience, Milan, ItalyLaboratory of Stem Cell Biology, Department of Cellular, Computational and Integrative Biology (CIBIO), Università degli Studi di Trento, Trento, ItalyEpsins are part of the internalization machinery pivotal to control clathrin-mediated endocytosis. Here, we report that epsin family members are expressed in mouse embryonic stem cells (mESCs) and that epsin1/2 knockdown alters both mESC exits from pluripotency and their differentiation. Furthermore, we show that epsin1/2 knockdown compromises the correct polarization and division of mESC-derived neural progenitors and their conversion into expandable radial glia-like neural stem cells. Finally, we provide evidence that Notch signaling is impaired following epsin1/2 knockdown and that experimental restoration of Notch signaling rescues the epsin-mediated phenotypes. We conclude that epsins contribute to control mESC exit from pluripotency and allow their neural differentiation by appropriate modulation of Notch signaling.http://dx.doi.org/10.1155/2019/4084351 |
| spellingShingle | Marina Cardano Jacopo Zasso Luca Ruggiero Giuseppina Di Giacomo Matteo Marcatili Ottavio Cremona Luciano Conti Epsins Regulate Mouse Embryonic Stem Cell Exit from Pluripotency and Neural Commitment by Controlling Notch Activation Stem Cells International |
| title | Epsins Regulate Mouse Embryonic Stem Cell Exit from Pluripotency and Neural Commitment by Controlling Notch Activation |
| title_full | Epsins Regulate Mouse Embryonic Stem Cell Exit from Pluripotency and Neural Commitment by Controlling Notch Activation |
| title_fullStr | Epsins Regulate Mouse Embryonic Stem Cell Exit from Pluripotency and Neural Commitment by Controlling Notch Activation |
| title_full_unstemmed | Epsins Regulate Mouse Embryonic Stem Cell Exit from Pluripotency and Neural Commitment by Controlling Notch Activation |
| title_short | Epsins Regulate Mouse Embryonic Stem Cell Exit from Pluripotency and Neural Commitment by Controlling Notch Activation |
| title_sort | epsins regulate mouse embryonic stem cell exit from pluripotency and neural commitment by controlling notch activation |
| url | http://dx.doi.org/10.1155/2019/4084351 |
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