PVT1 affects growth of glioma microvascular endothelial cells by negatively regulating miR-186
Vigorous angiogenesis is one of the reasons for the poor prognosis of glioma. A number of studies have shown that long non-coding RNA can affect a variety of biological behaviors of tumors. However, the influence of long non-coding RNAs on glioma vascular endothelial cells remains unclear. To simula...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2017-03-01
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| Series: | Tumor Biology |
| Online Access: | https://doi.org/10.1177/1010428317694326 |
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| _version_ | 1849248851195592704 |
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| author | Yawen Ma Ping Wang Yixue Xue Chengbin Qu Jian Zheng Xiaobai Liu Jun Ma Yunhui Liu |
| author_facet | Yawen Ma Ping Wang Yixue Xue Chengbin Qu Jian Zheng Xiaobai Liu Jun Ma Yunhui Liu |
| author_sort | Yawen Ma |
| collection | DOAJ |
| description | Vigorous angiogenesis is one of the reasons for the poor prognosis of glioma. A number of studies have shown that long non-coding RNA can affect a variety of biological behaviors of tumors. However, the influence of long non-coding RNAs on glioma vascular endothelial cells remains unclear. To simulate the glioma microenvironment, we applied glioma-conditioned medium to human cerebral microvascular endothelial cells. The long non-coding RNA PVT1 was found to be highly expressed in glioma vascular endothelial cells. Cell Counting Kit-8, migration, and tube formation assays showed that PVT1 overexpression promoted glioma vascular endothelial cells proliferation, migration, and angiogenesis. We also found that PVT1 overexpression upregulated the expression of the autophagy-related proteins Atg7 and Beclin1, which induced protective autophagy. Bioinformatics software and dual-luciferase system analysis confirmed that PVT1 acts by targeting miR-186. In addition, our study showed that miR-186 could target the 3′ untranslated region of Atg7 and Beclin1 to decrease their expression levels, thereby inhibiting glioma-conditioned human cerebral microvascular endothelial cell autophagy. In conclusion, PVT1 overexpression increased the expression of Atg7 and Beclin1 by targeting miR-186, which induced protective autophagy, thus promoting glioma vascular endothelial cell proliferation, migration, and angiogenesis. Therefore, PVT1 and miR-186 can provide new therapeutic targets for future anti-angiogenic treatment of glioma. |
| format | Article |
| id | doaj-art-bc0ddfe52f0a4557a32bf90dd5bc984e |
| institution | Kabale University |
| issn | 1423-0380 |
| language | English |
| publishDate | 2017-03-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Tumor Biology |
| spelling | doaj-art-bc0ddfe52f0a4557a32bf90dd5bc984e2025-08-20T03:57:44ZengSAGE PublishingTumor Biology1423-03802017-03-013910.1177/1010428317694326PVT1 affects growth of glioma microvascular endothelial cells by negatively regulating miR-186Yawen Ma0Ping Wang1Yixue Xue2Chengbin Qu3Jian Zheng4Xiaobai Liu5Jun Ma6Yunhui Liu7Liaoning Research Center for Translational Medicine in Nervous System Disease, Shenyang, People’s Republic of ChinaInstitute of Pathology and Pathophysiology, China Medical University, Shenyang, People’s Republic of ChinaInstitute of Pathology and Pathophysiology, China Medical University, Shenyang, People’s Republic of ChinaLiaoning Research Center for Translational Medicine in Nervous System Disease, Shenyang, People’s Republic of ChinaLiaoning Research Center for Translational Medicine in Nervous System Disease, Shenyang, People’s Republic of ChinaLiaoning Research Center for Translational Medicine in Nervous System Disease, Shenyang, People’s Republic of ChinaInstitute of Pathology and Pathophysiology, China Medical University, Shenyang, People’s Republic of ChinaLiaoning Research Center for Translational Medicine in Nervous System Disease, Shenyang, People’s Republic of ChinaVigorous angiogenesis is one of the reasons for the poor prognosis of glioma. A number of studies have shown that long non-coding RNA can affect a variety of biological behaviors of tumors. However, the influence of long non-coding RNAs on glioma vascular endothelial cells remains unclear. To simulate the glioma microenvironment, we applied glioma-conditioned medium to human cerebral microvascular endothelial cells. The long non-coding RNA PVT1 was found to be highly expressed in glioma vascular endothelial cells. Cell Counting Kit-8, migration, and tube formation assays showed that PVT1 overexpression promoted glioma vascular endothelial cells proliferation, migration, and angiogenesis. We also found that PVT1 overexpression upregulated the expression of the autophagy-related proteins Atg7 and Beclin1, which induced protective autophagy. Bioinformatics software and dual-luciferase system analysis confirmed that PVT1 acts by targeting miR-186. In addition, our study showed that miR-186 could target the 3′ untranslated region of Atg7 and Beclin1 to decrease their expression levels, thereby inhibiting glioma-conditioned human cerebral microvascular endothelial cell autophagy. In conclusion, PVT1 overexpression increased the expression of Atg7 and Beclin1 by targeting miR-186, which induced protective autophagy, thus promoting glioma vascular endothelial cell proliferation, migration, and angiogenesis. Therefore, PVT1 and miR-186 can provide new therapeutic targets for future anti-angiogenic treatment of glioma.https://doi.org/10.1177/1010428317694326 |
| spellingShingle | Yawen Ma Ping Wang Yixue Xue Chengbin Qu Jian Zheng Xiaobai Liu Jun Ma Yunhui Liu PVT1 affects growth of glioma microvascular endothelial cells by negatively regulating miR-186 Tumor Biology |
| title | PVT1 affects growth of glioma microvascular endothelial cells by negatively regulating miR-186 |
| title_full | PVT1 affects growth of glioma microvascular endothelial cells by negatively regulating miR-186 |
| title_fullStr | PVT1 affects growth of glioma microvascular endothelial cells by negatively regulating miR-186 |
| title_full_unstemmed | PVT1 affects growth of glioma microvascular endothelial cells by negatively regulating miR-186 |
| title_short | PVT1 affects growth of glioma microvascular endothelial cells by negatively regulating miR-186 |
| title_sort | pvt1 affects growth of glioma microvascular endothelial cells by negatively regulating mir 186 |
| url | https://doi.org/10.1177/1010428317694326 |
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