High spectral energy density all-fiber nanosecond pulsed 1.7 μm light source for photoacoustic microscopy
We present a high spectral energy density all-fiber nanosecond pulsed 1.7 μm light source specifically designed for photoacoustic microscopy (PAM). The system targets the 1st overtone absorption of C–H bonds near 1720 nm within the near-infrared-III (NIR-III) window, where lipids exhibit strong opti...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-08-01
|
| Series: | Photoacoustics |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213597925000679 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849423687485227008 |
|---|---|
| author | Seongjin Bak Sang Min Park Yuon Song Jeesu Kim Tae Won Nam Dong-Wook Han Chang-Seok Kim Soon-Woo Cho Brett E. Bouma Hwidon Lee |
| author_facet | Seongjin Bak Sang Min Park Yuon Song Jeesu Kim Tae Won Nam Dong-Wook Han Chang-Seok Kim Soon-Woo Cho Brett E. Bouma Hwidon Lee |
| author_sort | Seongjin Bak |
| collection | DOAJ |
| description | We present a high spectral energy density all-fiber nanosecond pulsed 1.7 μm light source specifically designed for photoacoustic microscopy (PAM). The system targets the 1st overtone absorption of C–H bonds near 1720 nm within the near-infrared-III (NIR-III) window, where lipids exhibit strong optical absorption, and tissues benefit from reduced scattering and high permissible fluence. To achieve narrow-linewidth, high pulse energy, and high pulse repetition rate (PRR), we developed a master oscillator fiber amplifier architecture based on stimulated Raman scattering. A 1589.80 nm Raman pump and a custom-built narrow-linewidth Raman seed laser were employed to generate spectrally pure 1719.44 nm pulses (∼0.10 nm linewidth). The proposed light source delivers nanosecond pulses (∼5 ns) with high pulse energy (≥2.2 μJ) and tunable PRRs up to 300 kHz, resulting in a spectral energy density of approximately 22 μJ/nm—significantly higher than that of conventional 1.7 μm light sources. Performance of the NIR-PAM system was validated through resolution testing with a 1951 USAF target, demonstrating a spatial resolution of approximately 4.14 μm and an axial resolution of approximately 85.5 μm. Phantom imaging of CH2-rich polymer films and ex vivo lipid-rich biological tissues confirmed the system’s high spatial fidelity and strong contrast for lipid-specific structures. This compact, stable, and spectrally refined light source with high spectral energy density can offer an effective solution for high-resolution, label-free molecular imaging and represents a promising platform for clinical photoacoustic imaging applications involving lipid detection and metabolic disease diagnostics. |
| format | Article |
| id | doaj-art-bc0ad741cd934bf4a29cabd17ff60798 |
| institution | Kabale University |
| issn | 2213-5979 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Photoacoustics |
| spelling | doaj-art-bc0ad741cd934bf4a29cabd17ff607982025-08-20T03:30:32ZengElsevierPhotoacoustics2213-59792025-08-014410074410.1016/j.pacs.2025.100744High spectral energy density all-fiber nanosecond pulsed 1.7 μm light source for photoacoustic microscopySeongjin Bak0Sang Min Park1Yuon Song2Jeesu Kim3Tae Won Nam4Dong-Wook Han5Chang-Seok Kim6Soon-Woo Cho7Brett E. Bouma8Hwidon Lee9Engineering Research Center for Color-Modulated Extra-Sensory Perception Technology, Pusan National University, Busan 46241, Republic of KoreaEngineering Research Center for Color-Modulated Extra-Sensory Perception Technology, Pusan National University, Busan 46241, Republic of KoreaDepartment of Cogno-Mechatronics Engineering, Pusan National University, Busan 46241, Republic of KoreaEngineering Research Center for Color-Modulated Extra-Sensory Perception Technology, Pusan National University, Busan 46241, Republic of Korea; Department of Cogno-Mechatronics Engineering, Pusan National University, Busan 46241, Republic of Korea; Corresponding authors at: Engineering Research Center for Color-Modulated Extra-Sensory Perception Technology, Pusan National University, Busan 46241, Republic of Korea.Department of Materials and Science and Engineering, Pusan National University, Busan 46241, Republic of KoreaDepartment of Cogno-Mechatronics Engineering, Pusan National University, Busan 46241, Republic of KoreaEngineering Research Center for Color-Modulated Extra-Sensory Perception Technology, Pusan National University, Busan 46241, Republic of Korea; Department of Cogno-Mechatronics Engineering, Pusan National University, Busan 46241, Republic of KoreaEngineering Research Center for Color-Modulated Extra-Sensory Perception Technology, Pusan National University, Busan 46241, Republic of Korea; Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA; Corresponding authors at: Engineering Research Center for Color-Modulated Extra-Sensory Perception Technology, Pusan National University, Busan 46241, Republic of Korea.Wellman Center for Photomedicine, Harvard Medical School and Massachusetts General Hospital, Boston, MA 02114, USAEngineering Research Center for Color-Modulated Extra-Sensory Perception Technology, Pusan National University, Busan 46241, Republic of Korea; Department of Cogno-Mechatronics Engineering, Pusan National University, Busan 46241, Republic of Korea; Corresponding authors at: Engineering Research Center for Color-Modulated Extra-Sensory Perception Technology, Pusan National University, Busan 46241, Republic of Korea.We present a high spectral energy density all-fiber nanosecond pulsed 1.7 μm light source specifically designed for photoacoustic microscopy (PAM). The system targets the 1st overtone absorption of C–H bonds near 1720 nm within the near-infrared-III (NIR-III) window, where lipids exhibit strong optical absorption, and tissues benefit from reduced scattering and high permissible fluence. To achieve narrow-linewidth, high pulse energy, and high pulse repetition rate (PRR), we developed a master oscillator fiber amplifier architecture based on stimulated Raman scattering. A 1589.80 nm Raman pump and a custom-built narrow-linewidth Raman seed laser were employed to generate spectrally pure 1719.44 nm pulses (∼0.10 nm linewidth). The proposed light source delivers nanosecond pulses (∼5 ns) with high pulse energy (≥2.2 μJ) and tunable PRRs up to 300 kHz, resulting in a spectral energy density of approximately 22 μJ/nm—significantly higher than that of conventional 1.7 μm light sources. Performance of the NIR-PAM system was validated through resolution testing with a 1951 USAF target, demonstrating a spatial resolution of approximately 4.14 μm and an axial resolution of approximately 85.5 μm. Phantom imaging of CH2-rich polymer films and ex vivo lipid-rich biological tissues confirmed the system’s high spatial fidelity and strong contrast for lipid-specific structures. This compact, stable, and spectrally refined light source with high spectral energy density can offer an effective solution for high-resolution, label-free molecular imaging and represents a promising platform for clinical photoacoustic imaging applications involving lipid detection and metabolic disease diagnostics.http://www.sciencedirect.com/science/article/pii/S2213597925000679High spectral energy densityAll-fiber nanosecond pulsed light sourceStimulated Raman scattering (SRS)1.7 µm light sourceNarrow-linewidthPhotoacoustic microscopy (PAM) |
| spellingShingle | Seongjin Bak Sang Min Park Yuon Song Jeesu Kim Tae Won Nam Dong-Wook Han Chang-Seok Kim Soon-Woo Cho Brett E. Bouma Hwidon Lee High spectral energy density all-fiber nanosecond pulsed 1.7 μm light source for photoacoustic microscopy Photoacoustics High spectral energy density All-fiber nanosecond pulsed light source Stimulated Raman scattering (SRS) 1.7 µm light source Narrow-linewidth Photoacoustic microscopy (PAM) |
| title | High spectral energy density all-fiber nanosecond pulsed 1.7 μm light source for photoacoustic microscopy |
| title_full | High spectral energy density all-fiber nanosecond pulsed 1.7 μm light source for photoacoustic microscopy |
| title_fullStr | High spectral energy density all-fiber nanosecond pulsed 1.7 μm light source for photoacoustic microscopy |
| title_full_unstemmed | High spectral energy density all-fiber nanosecond pulsed 1.7 μm light source for photoacoustic microscopy |
| title_short | High spectral energy density all-fiber nanosecond pulsed 1.7 μm light source for photoacoustic microscopy |
| title_sort | high spectral energy density all fiber nanosecond pulsed 1 7 μm light source for photoacoustic microscopy |
| topic | High spectral energy density All-fiber nanosecond pulsed light source Stimulated Raman scattering (SRS) 1.7 µm light source Narrow-linewidth Photoacoustic microscopy (PAM) |
| url | http://www.sciencedirect.com/science/article/pii/S2213597925000679 |
| work_keys_str_mv | AT seongjinbak highspectralenergydensityallfibernanosecondpulsed17mmlightsourceforphotoacousticmicroscopy AT sangminpark highspectralenergydensityallfibernanosecondpulsed17mmlightsourceforphotoacousticmicroscopy AT yuonsong highspectralenergydensityallfibernanosecondpulsed17mmlightsourceforphotoacousticmicroscopy AT jeesukim highspectralenergydensityallfibernanosecondpulsed17mmlightsourceforphotoacousticmicroscopy AT taewonnam highspectralenergydensityallfibernanosecondpulsed17mmlightsourceforphotoacousticmicroscopy AT dongwookhan highspectralenergydensityallfibernanosecondpulsed17mmlightsourceforphotoacousticmicroscopy AT changseokkim highspectralenergydensityallfibernanosecondpulsed17mmlightsourceforphotoacousticmicroscopy AT soonwoocho highspectralenergydensityallfibernanosecondpulsed17mmlightsourceforphotoacousticmicroscopy AT brettebouma highspectralenergydensityallfibernanosecondpulsed17mmlightsourceforphotoacousticmicroscopy AT hwidonlee highspectralenergydensityallfibernanosecondpulsed17mmlightsourceforphotoacousticmicroscopy |