Protocol refinement and inter- and intra-rater reliability assessment of ultrasound-based measurements of hamstring architecture, and echo intensity, and intra-rater reliability of shear wave elastography
Objectives: The increase in hamstring injuries highlights gaps in current understanding and prevention strategies. Hamstring architecture, quality, and mechanical properties influence force production, offering key insights into muscle health and function. This study aims to refine a standardized pr...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-06-01
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| Series: | WFUMB Ultrasound Open |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2949668325000011 |
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| author | Maria Belinda Cristina C. Fidel Jan Tyrone Cabrera Christine Grace V. Ogerio Johann Querijero Helen A. Banwell Consuelo B. Gonzalez-Suarez |
| author_facet | Maria Belinda Cristina C. Fidel Jan Tyrone Cabrera Christine Grace V. Ogerio Johann Querijero Helen A. Banwell Consuelo B. Gonzalez-Suarez |
| author_sort | Maria Belinda Cristina C. Fidel |
| collection | DOAJ |
| description | Objectives: The increase in hamstring injuries highlights gaps in current understanding and prevention strategies. Hamstring architecture, quality, and mechanical properties influence force production, offering key insights into muscle health and function. This study aims to refine a standardized protocol for hamstring image acquisition and digitization and to assess inter- and intra-rater reliability of measurements like fascicle length, pennation angle, muscle thickness, cross-sectional area, echo intensity, and shear wave elastography. Methods: This study had two phases: pilot and protocol refinement and intra- and inter-rater reliability of image digitization of fascicle length, pennation angle, muscle thickness, cross-sectional area, and echo intensity and intra-rater reliability of shear wave elastography using intra-class correlation coefficient. Results: This study developed and refined a hamstring image acquisition and digitization protocol, focusing on architecture, quality, and stiffness along its length at rest and during isometric contraction. Using Image J, intra-rater reliability for digitizing fascicle length, pennation angle, muscle thickness, cross-sectional area, and echo intensity was excellent. Inter-rater reliability ranged from moderate to excellent across all variables. Shear wave elastography showed moderate to excellent intra-rater reliability, with higher consistency during contraction than rest. Conclusion: The authors refined a standardized protocol for ultrasound imaging of hamstring architecture, quality, and stiffness, as well as digitization of fascicle length, pennation angle, muscle thickness, cross-sectional area, and echo intensity at rest and during isometric contraction using ImageJ. The protocol demonstrated moderate to excellent intra- and inter-rater reliability, with image markings further enhancing measurement consistency. |
| format | Article |
| id | doaj-art-bc06e7759358473e964f30fb1a843a59 |
| institution | OA Journals |
| issn | 2949-6683 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | WFUMB Ultrasound Open |
| spelling | doaj-art-bc06e7759358473e964f30fb1a843a592025-08-20T02:31:57ZengElsevierWFUMB Ultrasound Open2949-66832025-06-013110007910.1016/j.wfumbo.2025.100079Protocol refinement and inter- and intra-rater reliability assessment of ultrasound-based measurements of hamstring architecture, and echo intensity, and intra-rater reliability of shear wave elastographyMaria Belinda Cristina C. Fidel0Jan Tyrone Cabrera1Christine Grace V. Ogerio2Johann Querijero3Helen A. Banwell4Consuelo B. Gonzalez-Suarez5Graduate School, University of Santo Tomas, Espana Manila, Philippines; Corresponding author.Faculty Member, Department of Physical Medicine and Rehabilitation, Faculty of Medicine and Surgery, University of Santo Tomas, Espana Manila, PhilippinesDepartment of Physical Medicine and Rehabilitation, Our Lady of Lourdes Hospital, Manila, PhilippinesDepartment of Physical Medicine and Rehabilitation, Metro North Medical Center, Quezon City, PhilippinesAllied Health and Human Performance, University of South Australia, Adelaide, 5001, AustraliaFaculty Member, Department of Physical Medicine and Rehabilitation, Faculty of Medicine and Surgery, University of Santo Tomas, Espana Manila, Philippines; Department of Physical Medicine and Rehabilitation, Our Lady of Lourdes Hospital, Manila, PhilippinesObjectives: The increase in hamstring injuries highlights gaps in current understanding and prevention strategies. Hamstring architecture, quality, and mechanical properties influence force production, offering key insights into muscle health and function. This study aims to refine a standardized protocol for hamstring image acquisition and digitization and to assess inter- and intra-rater reliability of measurements like fascicle length, pennation angle, muscle thickness, cross-sectional area, echo intensity, and shear wave elastography. Methods: This study had two phases: pilot and protocol refinement and intra- and inter-rater reliability of image digitization of fascicle length, pennation angle, muscle thickness, cross-sectional area, and echo intensity and intra-rater reliability of shear wave elastography using intra-class correlation coefficient. Results: This study developed and refined a hamstring image acquisition and digitization protocol, focusing on architecture, quality, and stiffness along its length at rest and during isometric contraction. Using Image J, intra-rater reliability for digitizing fascicle length, pennation angle, muscle thickness, cross-sectional area, and echo intensity was excellent. Inter-rater reliability ranged from moderate to excellent across all variables. Shear wave elastography showed moderate to excellent intra-rater reliability, with higher consistency during contraction than rest. Conclusion: The authors refined a standardized protocol for ultrasound imaging of hamstring architecture, quality, and stiffness, as well as digitization of fascicle length, pennation angle, muscle thickness, cross-sectional area, and echo intensity at rest and during isometric contraction using ImageJ. The protocol demonstrated moderate to excellent intra- and inter-rater reliability, with image markings further enhancing measurement consistency.http://www.sciencedirect.com/science/article/pii/S2949668325000011HamstringUltrasoundReliability assessmentProtocol refinement |
| spellingShingle | Maria Belinda Cristina C. Fidel Jan Tyrone Cabrera Christine Grace V. Ogerio Johann Querijero Helen A. Banwell Consuelo B. Gonzalez-Suarez Protocol refinement and inter- and intra-rater reliability assessment of ultrasound-based measurements of hamstring architecture, and echo intensity, and intra-rater reliability of shear wave elastography WFUMB Ultrasound Open Hamstring Ultrasound Reliability assessment Protocol refinement |
| title | Protocol refinement and inter- and intra-rater reliability assessment of ultrasound-based measurements of hamstring architecture, and echo intensity, and intra-rater reliability of shear wave elastography |
| title_full | Protocol refinement and inter- and intra-rater reliability assessment of ultrasound-based measurements of hamstring architecture, and echo intensity, and intra-rater reliability of shear wave elastography |
| title_fullStr | Protocol refinement and inter- and intra-rater reliability assessment of ultrasound-based measurements of hamstring architecture, and echo intensity, and intra-rater reliability of shear wave elastography |
| title_full_unstemmed | Protocol refinement and inter- and intra-rater reliability assessment of ultrasound-based measurements of hamstring architecture, and echo intensity, and intra-rater reliability of shear wave elastography |
| title_short | Protocol refinement and inter- and intra-rater reliability assessment of ultrasound-based measurements of hamstring architecture, and echo intensity, and intra-rater reliability of shear wave elastography |
| title_sort | protocol refinement and inter and intra rater reliability assessment of ultrasound based measurements of hamstring architecture and echo intensity and intra rater reliability of shear wave elastography |
| topic | Hamstring Ultrasound Reliability assessment Protocol refinement |
| url | http://www.sciencedirect.com/science/article/pii/S2949668325000011 |
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