Soluble HLA-G and HLA-E Levels in Bone Marrow Plasma Samples Are Related to Disease Stage in Neuroblastoma Patients

The role of nonclassical HLA-class Ib molecules HLA-G and HLA-E in the progression of Neuroblastoma (NB), the most common pediatric extracranial solid tumor, has been characterized in the last years. Since BM infiltration by NB cells is an adverse prognostic factor, we have here analyzed for the fir...

Full description

Saved in:
Bibliographic Details
Main Authors: Fabio Morandi, Sarah Pozzi, Barbara Carlini, Loredana Amoroso, Vito Pistoia, Maria Valeria Corrias
Format: Article
Language:English
Published: Wiley 2016-01-01
Series:Journal of Immunology Research
Online Access:http://dx.doi.org/10.1155/2016/7465741
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1849685967851487232
author Fabio Morandi
Sarah Pozzi
Barbara Carlini
Loredana Amoroso
Vito Pistoia
Maria Valeria Corrias
author_facet Fabio Morandi
Sarah Pozzi
Barbara Carlini
Loredana Amoroso
Vito Pistoia
Maria Valeria Corrias
author_sort Fabio Morandi
collection DOAJ
description The role of nonclassical HLA-class Ib molecules HLA-G and HLA-E in the progression of Neuroblastoma (NB), the most common pediatric extracranial solid tumor, has been characterized in the last years. Since BM infiltration by NB cells is an adverse prognostic factor, we have here analyzed for the first time the concentration of soluble (s)HLA-G and HLA-E in bone marrow (BM) plasma samples from NB patients at diagnosis and healthy donors. sHLA-G and sHLA-E are present in BM plasma samples, and their levels were similar between NB patients and controls, thus suggesting that these molecules are physiologically released by resident or stromal BM cell populations. This hypothesis was supported by the finding that sHLA-G and sHLA-E levels did not correlate with BM infiltration and other adverse prognostic factors (MYCN amplification and age at diagnosis). In contrast, BM plasma levels of both molecules were higher in patients with metastatic disease than in patients with localized NB, thus suggesting that concentration of these molecules might be correlated with disease progression. The prognostic role of sHLA-G and sHLA-E concentration in the BM plasma for NB patients will be evaluated in future studies, by analyzing the clinical outcome of the same NB patients at follow-up.
format Article
id doaj-art-bc0290a1f64942eda48dc030f2694ee6
institution DOAJ
issn 2314-8861
2314-7156
language English
publishDate 2016-01-01
publisher Wiley
record_format Article
series Journal of Immunology Research
spelling doaj-art-bc0290a1f64942eda48dc030f2694ee62025-08-20T03:22:53ZengWileyJournal of Immunology Research2314-88612314-71562016-01-01201610.1155/2016/74657417465741Soluble HLA-G and HLA-E Levels in Bone Marrow Plasma Samples Are Related to Disease Stage in Neuroblastoma PatientsFabio Morandi0Sarah Pozzi1Barbara Carlini2Loredana Amoroso3Vito Pistoia4Maria Valeria Corrias5Laboratorio di Oncologia, Istituto Giannina Gaslini, Via Gaslini 5, 16147 Genoa, ItalyCentro Cellule Staminali, IRCCS AOU San Martino-IST, Largo R. Benzi 10, 16132 Genoa, ItalyLaboratorio di Oncologia, Istituto Giannina Gaslini, Via Gaslini 5, 16147 Genoa, ItalyUOC Oncologia, Istituto Giannina Gaslini, Via Gaslini 5, 16147 Genoa, ItalyLaboratorio di Oncologia, Istituto Giannina Gaslini, Via Gaslini 5, 16147 Genoa, ItalyLaboratorio di Oncologia, Istituto Giannina Gaslini, Via Gaslini 5, 16147 Genoa, ItalyThe role of nonclassical HLA-class Ib molecules HLA-G and HLA-E in the progression of Neuroblastoma (NB), the most common pediatric extracranial solid tumor, has been characterized in the last years. Since BM infiltration by NB cells is an adverse prognostic factor, we have here analyzed for the first time the concentration of soluble (s)HLA-G and HLA-E in bone marrow (BM) plasma samples from NB patients at diagnosis and healthy donors. sHLA-G and sHLA-E are present in BM plasma samples, and their levels were similar between NB patients and controls, thus suggesting that these molecules are physiologically released by resident or stromal BM cell populations. This hypothesis was supported by the finding that sHLA-G and sHLA-E levels did not correlate with BM infiltration and other adverse prognostic factors (MYCN amplification and age at diagnosis). In contrast, BM plasma levels of both molecules were higher in patients with metastatic disease than in patients with localized NB, thus suggesting that concentration of these molecules might be correlated with disease progression. The prognostic role of sHLA-G and sHLA-E concentration in the BM plasma for NB patients will be evaluated in future studies, by analyzing the clinical outcome of the same NB patients at follow-up.http://dx.doi.org/10.1155/2016/7465741
spellingShingle Fabio Morandi
Sarah Pozzi
Barbara Carlini
Loredana Amoroso
Vito Pistoia
Maria Valeria Corrias
Soluble HLA-G and HLA-E Levels in Bone Marrow Plasma Samples Are Related to Disease Stage in Neuroblastoma Patients
Journal of Immunology Research
title Soluble HLA-G and HLA-E Levels in Bone Marrow Plasma Samples Are Related to Disease Stage in Neuroblastoma Patients
title_full Soluble HLA-G and HLA-E Levels in Bone Marrow Plasma Samples Are Related to Disease Stage in Neuroblastoma Patients
title_fullStr Soluble HLA-G and HLA-E Levels in Bone Marrow Plasma Samples Are Related to Disease Stage in Neuroblastoma Patients
title_full_unstemmed Soluble HLA-G and HLA-E Levels in Bone Marrow Plasma Samples Are Related to Disease Stage in Neuroblastoma Patients
title_short Soluble HLA-G and HLA-E Levels in Bone Marrow Plasma Samples Are Related to Disease Stage in Neuroblastoma Patients
title_sort soluble hla g and hla e levels in bone marrow plasma samples are related to disease stage in neuroblastoma patients
url http://dx.doi.org/10.1155/2016/7465741
work_keys_str_mv AT fabiomorandi solublehlagandhlaelevelsinbonemarrowplasmasamplesarerelatedtodiseasestageinneuroblastomapatients
AT sarahpozzi solublehlagandhlaelevelsinbonemarrowplasmasamplesarerelatedtodiseasestageinneuroblastomapatients
AT barbaracarlini solublehlagandhlaelevelsinbonemarrowplasmasamplesarerelatedtodiseasestageinneuroblastomapatients
AT loredanaamoroso solublehlagandhlaelevelsinbonemarrowplasmasamplesarerelatedtodiseasestageinneuroblastomapatients
AT vitopistoia solublehlagandhlaelevelsinbonemarrowplasmasamplesarerelatedtodiseasestageinneuroblastomapatients
AT mariavaleriacorrias solublehlagandhlaelevelsinbonemarrowplasmasamplesarerelatedtodiseasestageinneuroblastomapatients