Integrating pharmacokinetics and network pharmacology to decipher the efficacy of Fufang Qinlan oral liquid on post infectious cough
Background and aim: Fufang Qinlan oral liquid (QLOL) is a traditional Chinese medicine formula used to treat fevers, sore throats and cough. This study was to evaluate QLOL's therapeutic effects on post infectious cough (PIC) and to investigate Q-markers and action mechanisms by integrating pha...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-05-01
|
| Series: | Journal of Traditional and Complementary Medicine |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2225411024000427 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850135708497346560 |
|---|---|
| author | Leyi Huang Yangyang Wang Xinyi Yue Fangfang Yu Tong Wu Yimeng Ge Chunmiao Wang Meihong Tong |
| author_facet | Leyi Huang Yangyang Wang Xinyi Yue Fangfang Yu Tong Wu Yimeng Ge Chunmiao Wang Meihong Tong |
| author_sort | Leyi Huang |
| collection | DOAJ |
| description | Background and aim: Fufang Qinlan oral liquid (QLOL) is a traditional Chinese medicine formula used to treat fevers, sore throats and cough. This study was to evaluate QLOL's therapeutic effects on post infectious cough (PIC) and to investigate Q-markers and action mechanisms by integrating pharmacokinetics and network pharmacology. Materials and methods: PIC rats were measured for cough frequency, organ index, pathological section of lung and inflammatory factors to evaluate the effects of QLOL. Pharmacokinetic analyses were performed using HPLC-QQQ-MS/MS to explore the possible Q-markers of QLOL and the changes of them in vivo. Network pharmacology was used to obtain potential important targets and action mechanisms of treating PIC. The possible hub genes were evaluated using QPCR. Results: The symptoms of cough and lung injury were significantly alleviated and IL-6 and IL-1β were significantly decreased after treatment of QLOL. 6 compounds were considered as possible Q-makers and their pharmacokinetic parameters were analyzed. The compound-target network was constructed to identify 53 important targets. Among them, HSPA8, HSP90AA1, HSP90AB1, YWHAZ, EGFR, ESR1 and EP300 were selected as the core targets because of high degree value and direct connection with inflammation or microbial infection. All 6 compounds had potently binding activity to core targets in molecular docking. The QPCR results showed the up-regulation of core targets expression in QLOL group compared with PIC rats, which validated the effects of QLOL and the accuracy of Q-markers selection. Conclusion: QLOL alleviated PIC symptoms through various targets and mechanisms. The potential Q-markers were baicalein, baicalin, oroxylin A-7-O-glucuronideloside, wogonoside, oroxylin A and forsythoside E. Taxonomy (classification by evise): the experimental approach. |
| format | Article |
| id | doaj-art-bc00bf0ef348418d9bb53a653f1369a0 |
| institution | OA Journals |
| issn | 2225-4110 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Traditional and Complementary Medicine |
| spelling | doaj-art-bc00bf0ef348418d9bb53a653f1369a02025-08-20T02:31:20ZengElsevierJournal of Traditional and Complementary Medicine2225-41102025-05-0115325026310.1016/j.jtcme.2024.04.006Integrating pharmacokinetics and network pharmacology to decipher the efficacy of Fufang Qinlan oral liquid on post infectious coughLeyi Huang0Yangyang Wang1Xinyi Yue2Fangfang Yu3Tong Wu4Yimeng Ge5Chunmiao Wang6Meihong Tong7National Key Laboratory of Lead Druggability Research, Shanghai Institute of Pharmaceutical Industry, China State Institute of Pharmaceutical Industry, 200437, Shanghai, ChinaNational Key Laboratory of Lead Druggability Research, Shanghai Institute of Pharmaceutical Industry, China State Institute of Pharmaceutical Industry, 200437, Shanghai, ChinaNational Key Laboratory of Lead Druggability Research, Shanghai Institute of Pharmaceutical Industry, China State Institute of Pharmaceutical Industry, 200437, Shanghai, ChinaNational Key Laboratory of Lead Druggability Research, Shanghai Institute of Pharmaceutical Industry, China State Institute of Pharmaceutical Industry, 200437, Shanghai, ChinaNational Key Laboratory of Lead Druggability Research, Shanghai Institute of Pharmaceutical Industry, China State Institute of Pharmaceutical Industry, 200437, Shanghai, China; Corresponding author. National Key Laboratory of Lead Druggability Research, Shanghai Institute of Pharmaceutical Industry, Shanghai, China.Heilongjiang Zhenbaodao Pharmaceutical CO., LTD., 158400, Hulin, Heilongjiang, ChinaHeilongjiang Zhenbaodao Pharmaceutical CO., LTD., 158400, Hulin, Heilongjiang, ChinaHeilongjiang Zhenbaodao Pharmaceutical CO., LTD., 158400, Hulin, Heilongjiang, ChinaBackground and aim: Fufang Qinlan oral liquid (QLOL) is a traditional Chinese medicine formula used to treat fevers, sore throats and cough. This study was to evaluate QLOL's therapeutic effects on post infectious cough (PIC) and to investigate Q-markers and action mechanisms by integrating pharmacokinetics and network pharmacology. Materials and methods: PIC rats were measured for cough frequency, organ index, pathological section of lung and inflammatory factors to evaluate the effects of QLOL. Pharmacokinetic analyses were performed using HPLC-QQQ-MS/MS to explore the possible Q-markers of QLOL and the changes of them in vivo. Network pharmacology was used to obtain potential important targets and action mechanisms of treating PIC. The possible hub genes were evaluated using QPCR. Results: The symptoms of cough and lung injury were significantly alleviated and IL-6 and IL-1β were significantly decreased after treatment of QLOL. 6 compounds were considered as possible Q-makers and their pharmacokinetic parameters were analyzed. The compound-target network was constructed to identify 53 important targets. Among them, HSPA8, HSP90AA1, HSP90AB1, YWHAZ, EGFR, ESR1 and EP300 were selected as the core targets because of high degree value and direct connection with inflammation or microbial infection. All 6 compounds had potently binding activity to core targets in molecular docking. The QPCR results showed the up-regulation of core targets expression in QLOL group compared with PIC rats, which validated the effects of QLOL and the accuracy of Q-markers selection. Conclusion: QLOL alleviated PIC symptoms through various targets and mechanisms. The potential Q-markers were baicalein, baicalin, oroxylin A-7-O-glucuronideloside, wogonoside, oroxylin A and forsythoside E. Taxonomy (classification by evise): the experimental approach.http://www.sciencedirect.com/science/article/pii/S2225411024000427Fufang qinlan oral liquidPost infectious coughTraditional Chinese medicine formulaPharmacokineticsPotential Q-markers |
| spellingShingle | Leyi Huang Yangyang Wang Xinyi Yue Fangfang Yu Tong Wu Yimeng Ge Chunmiao Wang Meihong Tong Integrating pharmacokinetics and network pharmacology to decipher the efficacy of Fufang Qinlan oral liquid on post infectious cough Journal of Traditional and Complementary Medicine Fufang qinlan oral liquid Post infectious cough Traditional Chinese medicine formula Pharmacokinetics Potential Q-markers |
| title | Integrating pharmacokinetics and network pharmacology to decipher the efficacy of Fufang Qinlan oral liquid on post infectious cough |
| title_full | Integrating pharmacokinetics and network pharmacology to decipher the efficacy of Fufang Qinlan oral liquid on post infectious cough |
| title_fullStr | Integrating pharmacokinetics and network pharmacology to decipher the efficacy of Fufang Qinlan oral liquid on post infectious cough |
| title_full_unstemmed | Integrating pharmacokinetics and network pharmacology to decipher the efficacy of Fufang Qinlan oral liquid on post infectious cough |
| title_short | Integrating pharmacokinetics and network pharmacology to decipher the efficacy of Fufang Qinlan oral liquid on post infectious cough |
| title_sort | integrating pharmacokinetics and network pharmacology to decipher the efficacy of fufang qinlan oral liquid on post infectious cough |
| topic | Fufang qinlan oral liquid Post infectious cough Traditional Chinese medicine formula Pharmacokinetics Potential Q-markers |
| url | http://www.sciencedirect.com/science/article/pii/S2225411024000427 |
| work_keys_str_mv | AT leyihuang integratingpharmacokineticsandnetworkpharmacologytodeciphertheefficacyoffufangqinlanoralliquidonpostinfectiouscough AT yangyangwang integratingpharmacokineticsandnetworkpharmacologytodeciphertheefficacyoffufangqinlanoralliquidonpostinfectiouscough AT xinyiyue integratingpharmacokineticsandnetworkpharmacologytodeciphertheefficacyoffufangqinlanoralliquidonpostinfectiouscough AT fangfangyu integratingpharmacokineticsandnetworkpharmacologytodeciphertheefficacyoffufangqinlanoralliquidonpostinfectiouscough AT tongwu integratingpharmacokineticsandnetworkpharmacologytodeciphertheefficacyoffufangqinlanoralliquidonpostinfectiouscough AT yimengge integratingpharmacokineticsandnetworkpharmacologytodeciphertheefficacyoffufangqinlanoralliquidonpostinfectiouscough AT chunmiaowang integratingpharmacokineticsandnetworkpharmacologytodeciphertheefficacyoffufangqinlanoralliquidonpostinfectiouscough AT meihongtong integratingpharmacokineticsandnetworkpharmacologytodeciphertheefficacyoffufangqinlanoralliquidonpostinfectiouscough |