Telomere-to-telomere gap-free genome assembly of Euchiloglanis kishinouyei

Abstract Euchiloglanis kishinouyei is a typical endemic torrent catfish found in the Jinsha River system of the upper Yangtze River in China. It inhabits fast-flowing streams with steep elevation gradients and has evolved unique biological adaptations to thrive in these extreme environments. A high-...

Full description

Saved in:
Bibliographic Details
Main Authors: Hao Wang, Xuefen Yang, Jisong Liu, Haizhou Wang, Yongyao Yu, Xiaojing Su, Yawen Fan, Haiping Liu, Ruibin Yang
Format: Article
Language:English
Published: Nature Portfolio 2025-05-01
Series:Scientific Data
Online Access:https://doi.org/10.1038/s41597-025-05068-8
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1849728418782904320
author Hao Wang
Xuefen Yang
Jisong Liu
Haizhou Wang
Yongyao Yu
Xiaojing Su
Yawen Fan
Haiping Liu
Ruibin Yang
author_facet Hao Wang
Xuefen Yang
Jisong Liu
Haizhou Wang
Yongyao Yu
Xiaojing Su
Yawen Fan
Haiping Liu
Ruibin Yang
author_sort Hao Wang
collection DOAJ
description Abstract Euchiloglanis kishinouyei is a typical endemic torrent catfish found in the Jinsha River system of the upper Yangtze River in China. It inhabits fast-flowing streams with steep elevation gradients and has evolved unique biological adaptations to thrive in these extreme environments. A high-quality genome provides key insights into the adaptive mechanisms driving its evolution in these harsh conditions. In this study, we successfully assembled the first telomere-to-telomere (T2T) genome of E. kishinouyei, marking the first T2T genome assembly of torrent catfish. The genome spans 886.74 Mb, anchored to 27 chromosomes, with over 99% coverage. The quality value (QV) and Benchmarking Universal Single-Copy Ortholog (BUSCO) scores were 46.96 and 98.50%, respectively, reflecting the high quality of the assembly. We identified repetitive elements accounting for 45.59% (404.23 Mb) of the genome and predicted 24,403 protein-coding genes, 94.37% of which were annotated. This high-fidelity genome assembly provides a valuable resource for future research and lays the foundation for exploring the ecological adaptation mechanisms and evolutionary biology of torrent catfish.
format Article
id doaj-art-bbef92d80be64ce9a16ad96d52ebbc82
institution DOAJ
issn 2052-4463
language English
publishDate 2025-05-01
publisher Nature Portfolio
record_format Article
series Scientific Data
spelling doaj-art-bbef92d80be64ce9a16ad96d52ebbc822025-08-20T03:09:34ZengNature PortfolioScientific Data2052-44632025-05-0112111110.1038/s41597-025-05068-8Telomere-to-telomere gap-free genome assembly of Euchiloglanis kishinouyeiHao Wang0Xuefen Yang1Jisong Liu2Haizhou Wang3Yongyao Yu4Xiaojing Su5Yawen Fan6Haiping Liu7Ruibin Yang8College of Fisheries, Huazhong Agricultural UniversityCollege of Fisheries, Huazhong Agricultural UniversityYebatan Branch of Huadian Jinshajiang Upstream Hydropower Development Co., Ltd.Yebatan Branch of Huadian Jinshajiang Upstream Hydropower Development Co., Ltd.College of Fisheries, Huazhong Agricultural UniversityCollege of Fisheries, Huazhong Agricultural UniversityCollege of Fisheries, Huazhong Agricultural UniversityCollege of Fisheries, Southwest UniversityCollege of Fisheries, Huazhong Agricultural UniversityAbstract Euchiloglanis kishinouyei is a typical endemic torrent catfish found in the Jinsha River system of the upper Yangtze River in China. It inhabits fast-flowing streams with steep elevation gradients and has evolved unique biological adaptations to thrive in these extreme environments. A high-quality genome provides key insights into the adaptive mechanisms driving its evolution in these harsh conditions. In this study, we successfully assembled the first telomere-to-telomere (T2T) genome of E. kishinouyei, marking the first T2T genome assembly of torrent catfish. The genome spans 886.74 Mb, anchored to 27 chromosomes, with over 99% coverage. The quality value (QV) and Benchmarking Universal Single-Copy Ortholog (BUSCO) scores were 46.96 and 98.50%, respectively, reflecting the high quality of the assembly. We identified repetitive elements accounting for 45.59% (404.23 Mb) of the genome and predicted 24,403 protein-coding genes, 94.37% of which were annotated. This high-fidelity genome assembly provides a valuable resource for future research and lays the foundation for exploring the ecological adaptation mechanisms and evolutionary biology of torrent catfish.https://doi.org/10.1038/s41597-025-05068-8
spellingShingle Hao Wang
Xuefen Yang
Jisong Liu
Haizhou Wang
Yongyao Yu
Xiaojing Su
Yawen Fan
Haiping Liu
Ruibin Yang
Telomere-to-telomere gap-free genome assembly of Euchiloglanis kishinouyei
Scientific Data
title Telomere-to-telomere gap-free genome assembly of Euchiloglanis kishinouyei
title_full Telomere-to-telomere gap-free genome assembly of Euchiloglanis kishinouyei
title_fullStr Telomere-to-telomere gap-free genome assembly of Euchiloglanis kishinouyei
title_full_unstemmed Telomere-to-telomere gap-free genome assembly of Euchiloglanis kishinouyei
title_short Telomere-to-telomere gap-free genome assembly of Euchiloglanis kishinouyei
title_sort telomere to telomere gap free genome assembly of euchiloglanis kishinouyei
url https://doi.org/10.1038/s41597-025-05068-8
work_keys_str_mv AT haowang telomeretotelomeregapfreegenomeassemblyofeuchiloglaniskishinouyei
AT xuefenyang telomeretotelomeregapfreegenomeassemblyofeuchiloglaniskishinouyei
AT jisongliu telomeretotelomeregapfreegenomeassemblyofeuchiloglaniskishinouyei
AT haizhouwang telomeretotelomeregapfreegenomeassemblyofeuchiloglaniskishinouyei
AT yongyaoyu telomeretotelomeregapfreegenomeassemblyofeuchiloglaniskishinouyei
AT xiaojingsu telomeretotelomeregapfreegenomeassemblyofeuchiloglaniskishinouyei
AT yawenfan telomeretotelomeregapfreegenomeassemblyofeuchiloglaniskishinouyei
AT haipingliu telomeretotelomeregapfreegenomeassemblyofeuchiloglaniskishinouyei
AT ruibinyang telomeretotelomeregapfreegenomeassemblyofeuchiloglaniskishinouyei