Stemness Characteristics of Periodontal Ligament Stem Cells from Donors and Multiple Sclerosis Patients: A Comparative Study
Multiple sclerosis (MS) is the most prevalent and progressive autoimmune disease that affects the central nervous system, and currently, no drug is available for the treatment. Stem cell therapy has received substantial attention in MS treatment. Recently, we demonstrated the immunosuppressive effec...
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| Language: | English |
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Wiley
2017-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2017/1606125 |
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| author | Francesca Diomede Thangavelu Soundara Rajan Marco D’Aurora Placido Bramanti Ilaria Merciaro Marco Marchisio Valentina Gatta Emanuela Mazzon Oriana Trubiani |
| author_facet | Francesca Diomede Thangavelu Soundara Rajan Marco D’Aurora Placido Bramanti Ilaria Merciaro Marco Marchisio Valentina Gatta Emanuela Mazzon Oriana Trubiani |
| author_sort | Francesca Diomede |
| collection | DOAJ |
| description | Multiple sclerosis (MS) is the most prevalent and progressive autoimmune disease that affects the central nervous system, and currently, no drug is available for the treatment. Stem cell therapy has received substantial attention in MS treatment. Recently, we demonstrated the immunosuppressive effects of mesenchymal stem cells derived from neural crest-originated human periodontal ligament tissue (hPDLSCs) in an in vivo model of MS. In the present study, we comparatively investigated the stemness properties of hPDLSCs derived from healthy donors and relapsing-remitting MS patients. Stem cell marker expression, cell proliferation, and differentiation capacity were studied. We found that both donor- and MS patient-derived hPDLSCs at early passage 2 showed similar expression of surface antigen markers and cell proliferation rate. Significant level of osteogenic, adipogenic, chondrogenic, and neurogenic differentiation capacities was observed in both donor- and MS patient-derived hPDLSCs. Interestingly, these cells maintained the stemness properties even at late passage 15. Senescence markers p16 and p21 expression was considerably enhanced in passage 15. Our results propose that hPDLSCs may serve as simple and potential autologous stem cell niche, which may help in personalized stem cell therapy for MS patients. |
| format | Article |
| id | doaj-art-bbe6204f804747f49d1caec676b59ebc |
| institution | DOAJ |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-bbe6204f804747f49d1caec676b59ebc2025-08-20T03:22:54ZengWileyStem Cells International1687-966X1687-96782017-01-01201710.1155/2017/16061251606125Stemness Characteristics of Periodontal Ligament Stem Cells from Donors and Multiple Sclerosis Patients: A Comparative StudyFrancesca Diomede0Thangavelu Soundara Rajan1Marco D’Aurora2Placido Bramanti3Ilaria Merciaro4Marco Marchisio5Valentina Gatta6Emanuela Mazzon7Oriana Trubiani8Stem Cells and Regenerative Medicine Laboratory, Department of Medical, Oral and Biotechnological Sciences, Università “G. d’Annunzio”, Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, ItalyIRCCS Centro Neurolesi “Bonino-Pulejo”, Via Provinciale Palermo, Contrada Casazza, 98124 Messina, ItalyDepartment of Psychological, Health and Territorial Sciences, School of Medicine, Università “G. d’Annunzio”, Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, ItalyIRCCS Centro Neurolesi “Bonino-Pulejo”, Via Provinciale Palermo, Contrada Casazza, 98124 Messina, ItalyStem Cells and Regenerative Medicine Laboratory, Department of Medical, Oral and Biotechnological Sciences, Università “G. d’Annunzio”, Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, ItalyDepartment of Medicine and Aging Science, Università “G. d’Annunzio”, Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, ItalyDepartment of Psychological, Health and Territorial Sciences, School of Medicine, Università “G. d’Annunzio”, Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, ItalyIRCCS Centro Neurolesi “Bonino-Pulejo”, Via Provinciale Palermo, Contrada Casazza, 98124 Messina, ItalyStem Cells and Regenerative Medicine Laboratory, Department of Medical, Oral and Biotechnological Sciences, Università “G. d’Annunzio”, Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, ItalyMultiple sclerosis (MS) is the most prevalent and progressive autoimmune disease that affects the central nervous system, and currently, no drug is available for the treatment. Stem cell therapy has received substantial attention in MS treatment. Recently, we demonstrated the immunosuppressive effects of mesenchymal stem cells derived from neural crest-originated human periodontal ligament tissue (hPDLSCs) in an in vivo model of MS. In the present study, we comparatively investigated the stemness properties of hPDLSCs derived from healthy donors and relapsing-remitting MS patients. Stem cell marker expression, cell proliferation, and differentiation capacity were studied. We found that both donor- and MS patient-derived hPDLSCs at early passage 2 showed similar expression of surface antigen markers and cell proliferation rate. Significant level of osteogenic, adipogenic, chondrogenic, and neurogenic differentiation capacities was observed in both donor- and MS patient-derived hPDLSCs. Interestingly, these cells maintained the stemness properties even at late passage 15. Senescence markers p16 and p21 expression was considerably enhanced in passage 15. Our results propose that hPDLSCs may serve as simple and potential autologous stem cell niche, which may help in personalized stem cell therapy for MS patients.http://dx.doi.org/10.1155/2017/1606125 |
| spellingShingle | Francesca Diomede Thangavelu Soundara Rajan Marco D’Aurora Placido Bramanti Ilaria Merciaro Marco Marchisio Valentina Gatta Emanuela Mazzon Oriana Trubiani Stemness Characteristics of Periodontal Ligament Stem Cells from Donors and Multiple Sclerosis Patients: A Comparative Study Stem Cells International |
| title | Stemness Characteristics of Periodontal Ligament Stem Cells from Donors and Multiple Sclerosis Patients: A Comparative Study |
| title_full | Stemness Characteristics of Periodontal Ligament Stem Cells from Donors and Multiple Sclerosis Patients: A Comparative Study |
| title_fullStr | Stemness Characteristics of Periodontal Ligament Stem Cells from Donors and Multiple Sclerosis Patients: A Comparative Study |
| title_full_unstemmed | Stemness Characteristics of Periodontal Ligament Stem Cells from Donors and Multiple Sclerosis Patients: A Comparative Study |
| title_short | Stemness Characteristics of Periodontal Ligament Stem Cells from Donors and Multiple Sclerosis Patients: A Comparative Study |
| title_sort | stemness characteristics of periodontal ligament stem cells from donors and multiple sclerosis patients a comparative study |
| url | http://dx.doi.org/10.1155/2017/1606125 |
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