Expression and significance of phospholipase A2 receptor and thrombospondin type-1 domain 7A in idiopathic membranous nephropathy
Objective To investigate expression and significance of phospholipase A2 receptor(PLA2 R) and thrombospondin type-1 domain 7 A(THSD7 A) in idiopathic membranous nephropathy(IMN). Methods Immunohistochemistry was used to detect the expression of glomerular phospholipase A2 receptor(G-PLA2 R) and glom...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | zho |
| Published: |
Editorial Department of Journal of Clinical Nephrology
2019-01-01
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| Series: | Linchuang shenzangbing zazhi |
| Subjects: | |
| Online Access: | http://www.lcszb.com/thesisDetails?columnId=57911119&Fpath=home&index=0 |
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| Summary: | Objective To investigate expression and significance of phospholipase A2 receptor(PLA2 R) and thrombospondin type-1 domain 7 A(THSD7 A) in idiopathic membranous nephropathy(IMN). Methods Immunohistochemistry was used to detect the expression of glomerular phospholipase A2 receptor(G-PLA2 R) and glomerular thrombospondin type-1 domain 7 A(G-THSD7 A) in the renal tissue in MN patients, and serum PLA2 R(S-PLA2 R) and serum thrombospondin type-1 domain 7 A(S-THSD7 A) levels were detected with enzyme-linked immunosorbent assay(ELISA) so as to evaluate the diagnostic value for IMN. Results The positive expression rate of G-PLA2 R in 153 IMN patients included in this study was 56.2%, the positive rate of IgG4 in the G-PLA2 R positive group was 69.8%; the positive expression rate of G-THSD7 A was 4.6%, and the positive rate of IgG4 in the G-THSD7 A positive group was 85.7%. Detection of serum S-PLA2 R and S-THSD7 A in 36 IMN patients showed that the positive rates of S-PLA2 R and S-THSD7 A were 69.4% and 5.6% respectively. The sensitivity values of G-PLA2 R, G-THSD7 A, G-PLA2 R+IgG4, and G-THSD7 A+IgG4 for the diagnosis of IMN were 56.2%, 4.6%, 39.2%, and 3.9%, respectively; and the specificity values were 65.2%, 100%, 78.3%, and 100%, respectively. Conclusions Glomerular PLA2 R and serum PLA2 R have a certain positive expression rate in IMN patients, and are specific diagnostic indices for IMN. Its combination with glomerular IgG4 can improve the diagnostic specificity. The expression rates of glomerular THSD7 A and serum THSD7 A are very low in IMN patients, and can be used as specific and supplementary diagnostic indicators for IMN. |
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| ISSN: | 1671-2390 |