Vibration synergistically enhances IL-1β and TNF-α in compressed human periodontal ligament cells in the frequency-dependent manner
Objectives: To investigate whether mechanical vibration at 30 or 60 Hz combined with compressive force alter IL-1β and TNF-α expression in human periodontal ligament (hPDL) cells. Methods: hPDL cells isolated from the roots of first premolar teeth extracted from four independent donors were cultured...
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| Format: | Article |
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Elsevier
2020-10-01
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| Series: | Journal of Oral Biology and Craniofacial Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2212426820300737 |
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| author | Sutiwa Benjakul Boontarika Unat Peungchaleoy Thammanichanon Chidchanok Leethanakul |
| author_facet | Sutiwa Benjakul Boontarika Unat Peungchaleoy Thammanichanon Chidchanok Leethanakul |
| author_sort | Sutiwa Benjakul |
| collection | DOAJ |
| description | Objectives: To investigate whether mechanical vibration at 30 or 60 Hz combined with compressive force alter IL-1β and TNF-α expression in human periodontal ligament (hPDL) cells. Methods: hPDL cells isolated from the roots of first premolar teeth extracted from four independent donors were cultured and exposed to vibration (0.3 g, 20 min per cycle, every 24 h for 3 cycles) at 30 or 60 Hz (V30 or V60), 2.0 g/cm2 compressive force for 2 days (CF), or a combination of compressive force and vibration at 30 Hz or 60 Hz (V30CF or V60CF). Quantitative real-time polymerase chain reaction (qPCR) and enzyme-linked immunosorbent assays (ELISAs) were used to determine IL-1β and TNF-α mRNA and protein, respectively. Results: The levels of IL-1β and TNF-α did not alter in groups V30 and V60. While, they were upregulated in groups CF, V30CF and V60CF. In addition, IL-1β mRNA and TNF-α mRNA and protein were expressed at significantly higher levels in group V30CF compared to CF group. However, IL-1β protein levels between V30CF and CF groups did not reach statistical significance. Conclusions: 30 Hz vibration had the synergistic effects with compressive force on the upregulation of IL-1β mRNA and TNF-α mRNA and protein in PDL cells, while 60 Hz vibration did not have this synergistic effect. |
| format | Article |
| id | doaj-art-bbe040f6b63e4cf2b443add6e768248a |
| institution | DOAJ |
| issn | 2212-4268 |
| language | English |
| publishDate | 2020-10-01 |
| publisher | Elsevier |
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| series | Journal of Oral Biology and Craniofacial Research |
| spelling | doaj-art-bbe040f6b63e4cf2b443add6e768248a2025-08-20T02:50:27ZengElsevierJournal of Oral Biology and Craniofacial Research2212-42682020-10-0110441241610.1016/j.jobcr.2020.06.005Vibration synergistically enhances IL-1β and TNF-α in compressed human periodontal ligament cells in the frequency-dependent mannerSutiwa Benjakul0Boontarika Unat1Peungchaleoy Thammanichanon2Chidchanok Leethanakul3Orthodontic Section, Faculty of Dentistry, Thammasat University, ThailandOrthodontic Section, Department of Preventive Dentistry, Faculty of Dentistry, Prince of Songkla University, ThailandSchool of Adolescent Oral Health, Institute of Dentistry, Suranaree University of Technology, ThailandOrthodontic Section, Department of Preventive Dentistry, Faculty of Dentistry, Prince of Songkla University, Thailand; Corresponding author. Hat Yai, Songkhla 90112, Thailand.Objectives: To investigate whether mechanical vibration at 30 or 60 Hz combined with compressive force alter IL-1β and TNF-α expression in human periodontal ligament (hPDL) cells. Methods: hPDL cells isolated from the roots of first premolar teeth extracted from four independent donors were cultured and exposed to vibration (0.3 g, 20 min per cycle, every 24 h for 3 cycles) at 30 or 60 Hz (V30 or V60), 2.0 g/cm2 compressive force for 2 days (CF), or a combination of compressive force and vibration at 30 Hz or 60 Hz (V30CF or V60CF). Quantitative real-time polymerase chain reaction (qPCR) and enzyme-linked immunosorbent assays (ELISAs) were used to determine IL-1β and TNF-α mRNA and protein, respectively. Results: The levels of IL-1β and TNF-α did not alter in groups V30 and V60. While, they were upregulated in groups CF, V30CF and V60CF. In addition, IL-1β mRNA and TNF-α mRNA and protein were expressed at significantly higher levels in group V30CF compared to CF group. However, IL-1β protein levels between V30CF and CF groups did not reach statistical significance. Conclusions: 30 Hz vibration had the synergistic effects with compressive force on the upregulation of IL-1β mRNA and TNF-α mRNA and protein in PDL cells, while 60 Hz vibration did not have this synergistic effect.http://www.sciencedirect.com/science/article/pii/S2212426820300737Accelerated tooth movementBiomarkersCytokinesVibratory stimulation |
| spellingShingle | Sutiwa Benjakul Boontarika Unat Peungchaleoy Thammanichanon Chidchanok Leethanakul Vibration synergistically enhances IL-1β and TNF-α in compressed human periodontal ligament cells in the frequency-dependent manner Journal of Oral Biology and Craniofacial Research Accelerated tooth movement Biomarkers Cytokines Vibratory stimulation |
| title | Vibration synergistically enhances IL-1β and TNF-α in compressed human periodontal ligament cells in the frequency-dependent manner |
| title_full | Vibration synergistically enhances IL-1β and TNF-α in compressed human periodontal ligament cells in the frequency-dependent manner |
| title_fullStr | Vibration synergistically enhances IL-1β and TNF-α in compressed human periodontal ligament cells in the frequency-dependent manner |
| title_full_unstemmed | Vibration synergistically enhances IL-1β and TNF-α in compressed human periodontal ligament cells in the frequency-dependent manner |
| title_short | Vibration synergistically enhances IL-1β and TNF-α in compressed human periodontal ligament cells in the frequency-dependent manner |
| title_sort | vibration synergistically enhances il 1β and tnf α in compressed human periodontal ligament cells in the frequency dependent manner |
| topic | Accelerated tooth movement Biomarkers Cytokines Vibratory stimulation |
| url | http://www.sciencedirect.com/science/article/pii/S2212426820300737 |
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