CPK28-mediated Ca2+ signaling regulates STOP1 localization and accumulation to facilitate plant aluminum resistance
Abstract The transcription factor SENSITIVE TO PROTON RHIZOTOXICITY 1 (STOP1) functions as a crucial integrator of plant responses to various stresses, including aluminum (Al) stress. Its stability and accumulation are modulated by stress-specific post-translational mechanisms such as phosphorylatio...
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| Format: | Article |
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Nature Portfolio
2025-06-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-60427-8 |
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| author | Yingtang Ma Hailiang Zheng Ina Schmitz-Thom Jiawen Wang Fanglin Zhou Chongyang Li Yaling Zhang Yiqiu Cheng Daisuke Miki Jörg Kudla Chao-Feng Huang |
| author_facet | Yingtang Ma Hailiang Zheng Ina Schmitz-Thom Jiawen Wang Fanglin Zhou Chongyang Li Yaling Zhang Yiqiu Cheng Daisuke Miki Jörg Kudla Chao-Feng Huang |
| author_sort | Yingtang Ma |
| collection | DOAJ |
| description | Abstract The transcription factor SENSITIVE TO PROTON RHIZOTOXICITY 1 (STOP1) functions as a crucial integrator of plant responses to various stresses, including aluminum (Al) stress. Its stability and accumulation are modulated by stress-specific post-translational mechanisms such as phosphorylation and ubiquitination. However, the upstream signaling mechanisms governing these modifications remain poorly understood. Here, we reveal that Ca2+ signaling and Ca2+-dependent phosphorylation are essential for Al stress-responsive regulation of STOP1. Al exposure specifically induces rapid, spatio-temporally defined biphasic Ca2+ signals in Arabidopsis roots and concomitantly activates the Ca2+-dependent kinase CPK28. Al-activated CPK28 phosphorylates STOP1 at Ser163, a modification that promotes the nuclear localization of STOP1 and prevents its degradation by inhibiting its interaction with the F-box protein RAE1. This phosphorylation enhances STOP1 accumulation and Al resistance. Our findings identify Ser163 phosphorylation as a key molecular switch and establish a Ca2+-CPK28-STOP1 signaling axis critical for plant adaptation to Al stress. |
| format | Article |
| id | doaj-art-bbd663b8407d44edbd87491d4620a8b1 |
| institution | DOAJ |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-bbd663b8407d44edbd87491d4620a8b12025-08-20T03:10:34ZengNature PortfolioNature Communications2041-17232025-06-0116111810.1038/s41467-025-60427-8CPK28-mediated Ca2+ signaling regulates STOP1 localization and accumulation to facilitate plant aluminum resistanceYingtang Ma0Hailiang Zheng1Ina Schmitz-Thom2Jiawen Wang3Fanglin Zhou4Chongyang Li5Yaling Zhang6Yiqiu Cheng7Daisuke Miki8Jörg Kudla9Chao-Feng Huang10State Key Laboratory of Plant Trait Design, Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of SciencesState Key Laboratory of Plant Trait Design, Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of SciencesInstitut für Biologie und Biotechnologie der Pflanzen, Universität MünsterState Key Laboratory of Plant Trait Design, Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of SciencesState Key Laboratory of Plant Trait Design, Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of SciencesState Key Laboratory of Plant Trait Design, Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of SciencesState Key Laboratory of Plant Trait Design, Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of SciencesState Key Laboratory of Plant Trait Design, Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of SciencesState Key Laboratory of Plant Trait Design, Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of SciencesInstitut für Biologie und Biotechnologie der Pflanzen, Universität MünsterState Key Laboratory of Plant Trait Design, Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of SciencesAbstract The transcription factor SENSITIVE TO PROTON RHIZOTOXICITY 1 (STOP1) functions as a crucial integrator of plant responses to various stresses, including aluminum (Al) stress. Its stability and accumulation are modulated by stress-specific post-translational mechanisms such as phosphorylation and ubiquitination. However, the upstream signaling mechanisms governing these modifications remain poorly understood. Here, we reveal that Ca2+ signaling and Ca2+-dependent phosphorylation are essential for Al stress-responsive regulation of STOP1. Al exposure specifically induces rapid, spatio-temporally defined biphasic Ca2+ signals in Arabidopsis roots and concomitantly activates the Ca2+-dependent kinase CPK28. Al-activated CPK28 phosphorylates STOP1 at Ser163, a modification that promotes the nuclear localization of STOP1 and prevents its degradation by inhibiting its interaction with the F-box protein RAE1. This phosphorylation enhances STOP1 accumulation and Al resistance. Our findings identify Ser163 phosphorylation as a key molecular switch and establish a Ca2+-CPK28-STOP1 signaling axis critical for plant adaptation to Al stress.https://doi.org/10.1038/s41467-025-60427-8 |
| spellingShingle | Yingtang Ma Hailiang Zheng Ina Schmitz-Thom Jiawen Wang Fanglin Zhou Chongyang Li Yaling Zhang Yiqiu Cheng Daisuke Miki Jörg Kudla Chao-Feng Huang CPK28-mediated Ca2+ signaling regulates STOP1 localization and accumulation to facilitate plant aluminum resistance Nature Communications |
| title | CPK28-mediated Ca2+ signaling regulates STOP1 localization and accumulation to facilitate plant aluminum resistance |
| title_full | CPK28-mediated Ca2+ signaling regulates STOP1 localization and accumulation to facilitate plant aluminum resistance |
| title_fullStr | CPK28-mediated Ca2+ signaling regulates STOP1 localization and accumulation to facilitate plant aluminum resistance |
| title_full_unstemmed | CPK28-mediated Ca2+ signaling regulates STOP1 localization and accumulation to facilitate plant aluminum resistance |
| title_short | CPK28-mediated Ca2+ signaling regulates STOP1 localization and accumulation to facilitate plant aluminum resistance |
| title_sort | cpk28 mediated ca2 signaling regulates stop1 localization and accumulation to facilitate plant aluminum resistance |
| url | https://doi.org/10.1038/s41467-025-60427-8 |
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