Kinetics of soil urease affected by phenyl phosphorodiamidate
By simulation, the effects of the inhibitor phenyl phosphorodiamidate (PPD) on the kinetics of urease were studied in the main 4 soils of Northeast China at 10, 20℃ and 30 ℃. The results show that effects of PPD on kinetic parameters of soil urease were significantly influenced by soil fertility, in...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2008-07-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2008.04.013 |
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| Summary: | By simulation, the effects of the inhibitor phenyl phosphorodiamidate (PPD) on the kinetics of urease were studied in the main 4 soils of Northeast China at 10, 20℃ and 30 ℃. The results show that effects of PPD on kinetic parameters of soil urease were significantly influenced by soil fertility, incubation time, incubation temperature and their interactions. Compared with control, PPD increased the K<sub>m</sub> but decreased the V<sub>max</sub> and V<sub>max</sub>/K<sub>m</sub> of soil urease, suggesting that the action mechanism of PPD is of competitive inhibition. With incubation time increasing, the kinetic parameters, K<sub>m</sub>, V<sub>max</sub> and V<sub>max</sub>/Km, of PPD treatment increased, suggesting the effectiveness of PPD on soil urease gradually weakened. The K<sub>m</sub> and V<sub>max</sub> increased with increasing incubation temperature, while V<sub>max</sub>/K<sub>m</sub> values had no definite change pattern. The kinetics of soil urease differed with soil types. The urease in black soil and albic soil had larger values of Km and V<sub>max</sub> than those in brown soil and cinnamon soil, but V<sub>max</sub>/K<sub>m</sub> had no significant differences, indicating that the kinetic parameters of soil urease were significantly influenced by soil type. Statistical analysis presented that there were significant correlations between the kinetic parameters of soil urease and the physiochemical characteristics of test soils. |
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| ISSN: | 1008-9209 2097-5155 |