Methionine Promoted the Growth of Golden Pompano by Activating Intestinal Transport and TOR Signaling Pathway
This experiment aimed to study the effects of methionine (Met) on growth performance, intestinal structure and transport, Met metabolism, and target of rapamycin (TOR) signaling pathway in golden pompano (Trachinotus ovatus). Fish (9.12 ± 0.03 g) were fed with six diets with 0.79%, 0.90%, 1.01%, 1.1...
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| Format: | Article |
| Language: | English |
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Wiley
2025-01-01
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| Series: | Aquaculture Nutrition |
| Online Access: | http://dx.doi.org/10.1155/anu/8592097 |
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| author | Zhikang Song Yuanyuan Zhou Yingtao Li Zhiwen Chen Xingyuan Liu Xinting Liu Zhenzhu Sun Chaoxia Ye |
| author_facet | Zhikang Song Yuanyuan Zhou Yingtao Li Zhiwen Chen Xingyuan Liu Xinting Liu Zhenzhu Sun Chaoxia Ye |
| author_sort | Zhikang Song |
| collection | DOAJ |
| description | This experiment aimed to study the effects of methionine (Met) on growth performance, intestinal structure and transport, Met metabolism, and target of rapamycin (TOR) signaling pathway in golden pompano (Trachinotus ovatus). Fish (9.12 ± 0.03 g) were fed with six diets with 0.79%, 0.90%, 1.01%, 1.12%, 1.23%, and 1.34% DL-Met for 8 weeks. Our study showed that Met supplementation significantly increased fish growth, villus length, crypt depth, and the mRNA levels of intestinal amino acid transporters including asc-type amino acid transporter 1 (asc-1), sodium-dependent neutral amino acid transporter B (asct2), and cationic amino acid transporter1 (cat1) (p<0.05). Liver histological analysis revealed that Met addition improved cell swelling, nuclear migration, and hepatic vacuolation. Appropriate Met supplementation significantly increased mRNA level of key genes (methionine adenosyl transferase [mat], cystathionine beta-synthase [cbs], cystathionine γ-lyase [cse], and cysteine dioxygenase [cdo]) involved in transmethylation, transsulfuration metabolism, and taurine synthesis pathways. The level of 1.12%–1.23%, 1.12%, and 1.23% Met significantly upregulated the mRNA expression of S-adenosyl methionine/target of rapamycin (samtor), regulatory-associated protein of TOR (raptor), and ribosomal protein s6 (s6), respectively. The protein levels of protein kinase B (AKT), TOR, p-TOR, S6K, p-S6K, and p-S6 increased firstly and then decreased with increasing dietary Met supplementation. In conclusion, Met supplementation may promote growth of golden pompano by improving intestinal structure and amino acid transport, increasing transmethylation and transsulfuration metabolism, and activating TOR signaling pathways through SAMTOR and AKT. |
| format | Article |
| id | doaj-art-bbd243f3c7624fe0a57967da5b864ed1 |
| institution | Kabale University |
| issn | 1365-2095 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Aquaculture Nutrition |
| spelling | doaj-art-bbd243f3c7624fe0a57967da5b864ed12025-08-20T03:47:04ZengWileyAquaculture Nutrition1365-20952025-01-01202510.1155/anu/8592097Methionine Promoted the Growth of Golden Pompano by Activating Intestinal Transport and TOR Signaling PathwayZhikang Song0Yuanyuan Zhou1Yingtao Li2Zhiwen Chen3Xingyuan Liu4Xinting Liu5Zhenzhu Sun6Chaoxia Ye7Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, School of Life ScienceGuangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, School of Life ScienceGuangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, School of Life ScienceGuangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, School of Life ScienceGuangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, School of Life ScienceGuangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, School of Life ScienceSchool of Biology and AgricultureGuangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, School of Life ScienceThis experiment aimed to study the effects of methionine (Met) on growth performance, intestinal structure and transport, Met metabolism, and target of rapamycin (TOR) signaling pathway in golden pompano (Trachinotus ovatus). Fish (9.12 ± 0.03 g) were fed with six diets with 0.79%, 0.90%, 1.01%, 1.12%, 1.23%, and 1.34% DL-Met for 8 weeks. Our study showed that Met supplementation significantly increased fish growth, villus length, crypt depth, and the mRNA levels of intestinal amino acid transporters including asc-type amino acid transporter 1 (asc-1), sodium-dependent neutral amino acid transporter B (asct2), and cationic amino acid transporter1 (cat1) (p<0.05). Liver histological analysis revealed that Met addition improved cell swelling, nuclear migration, and hepatic vacuolation. Appropriate Met supplementation significantly increased mRNA level of key genes (methionine adenosyl transferase [mat], cystathionine beta-synthase [cbs], cystathionine γ-lyase [cse], and cysteine dioxygenase [cdo]) involved in transmethylation, transsulfuration metabolism, and taurine synthesis pathways. The level of 1.12%–1.23%, 1.12%, and 1.23% Met significantly upregulated the mRNA expression of S-adenosyl methionine/target of rapamycin (samtor), regulatory-associated protein of TOR (raptor), and ribosomal protein s6 (s6), respectively. The protein levels of protein kinase B (AKT), TOR, p-TOR, S6K, p-S6K, and p-S6 increased firstly and then decreased with increasing dietary Met supplementation. In conclusion, Met supplementation may promote growth of golden pompano by improving intestinal structure and amino acid transport, increasing transmethylation and transsulfuration metabolism, and activating TOR signaling pathways through SAMTOR and AKT.http://dx.doi.org/10.1155/anu/8592097 |
| spellingShingle | Zhikang Song Yuanyuan Zhou Yingtao Li Zhiwen Chen Xingyuan Liu Xinting Liu Zhenzhu Sun Chaoxia Ye Methionine Promoted the Growth of Golden Pompano by Activating Intestinal Transport and TOR Signaling Pathway Aquaculture Nutrition |
| title | Methionine Promoted the Growth of Golden Pompano by Activating Intestinal Transport and TOR Signaling Pathway |
| title_full | Methionine Promoted the Growth of Golden Pompano by Activating Intestinal Transport and TOR Signaling Pathway |
| title_fullStr | Methionine Promoted the Growth of Golden Pompano by Activating Intestinal Transport and TOR Signaling Pathway |
| title_full_unstemmed | Methionine Promoted the Growth of Golden Pompano by Activating Intestinal Transport and TOR Signaling Pathway |
| title_short | Methionine Promoted the Growth of Golden Pompano by Activating Intestinal Transport and TOR Signaling Pathway |
| title_sort | methionine promoted the growth of golden pompano by activating intestinal transport and tor signaling pathway |
| url | http://dx.doi.org/10.1155/anu/8592097 |
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