Ultrasound-assisted extraction of triterpenoids from Chaenomeles speciosa leaves: Process optimization, adsorptive enrichment, chemical profiling, and protection against ulcerative colitis
For the valorization of Chaenomeles speciosa leaves, this study focused on extraction, enrichment, chemical profiling, and investigation of the biological activity of its abundant triterpenoid components. Initially, the total triterpenoids in C. speciosa leaves were extracted by ultrasonic-assisted...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-12-01
|
| Series: | Ultrasonics Sonochemistry |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1350417724003857 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850130412081250304 |
|---|---|
| author | Mengyang Hou Jingchun Shi Chengyuan Lin Lin Zhu Zhaoxiang Bian |
| author_facet | Mengyang Hou Jingchun Shi Chengyuan Lin Lin Zhu Zhaoxiang Bian |
| author_sort | Mengyang Hou |
| collection | DOAJ |
| description | For the valorization of Chaenomeles speciosa leaves, this study focused on extraction, enrichment, chemical profiling, and investigation of the biological activity of its abundant triterpenoid components. Initially, the total triterpenoids in C. speciosa leaves were extracted by ultrasonic-assisted extraction (UAE) method, with the extraction process optimized through response surface methodology (RSM). Under the optimal conditions of extraction solvent 93 % EtOH, ultrasound power 390 W, extraction time 30 min, extraction temperature 70 °C, liquid-to-solid ratio 25 mL/g, and 2 extraction cycles, the maximum total triterpenoids yield (TTY) reached 36.77 ± 0.40 mg/g. The total triterpenoids in the crude extract were subsequently enriched by X-5 resin column chromatography, resulting in a fourfold increase in purity, reaching 73.27 ± 0.84 %. Thirteen compounds in the triterpenoid-rich fraction (TRF) were identified through UPLC–QTOF-MS/MS, and five major triterpenoids (oleanolic acid, ursolic acid, betulinic acid, maslinic acid, and pomolic acid) were simultaneously quantified by HPLC–QQQ-MS. Furthermore, TRF demonstrated a notable amelioration against dextran sodium sulfate (DSS)-induced ulcerative colitis in mice, indicating its promise as a potent intervention for this condition. In summary, this study will contribute to enhancing the utilization efficiency of Chaenomeles speciosa leaves. |
| format | Article |
| id | doaj-art-bbccbfb4dbba4c22b96d527538d47a12 |
| institution | OA Journals |
| issn | 1350-4177 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ultrasonics Sonochemistry |
| spelling | doaj-art-bbccbfb4dbba4c22b96d527538d47a122025-08-20T02:32:42ZengElsevierUltrasonics Sonochemistry1350-41772024-12-0111110713610.1016/j.ultsonch.2024.107136Ultrasound-assisted extraction of triterpenoids from Chaenomeles speciosa leaves: Process optimization, adsorptive enrichment, chemical profiling, and protection against ulcerative colitisMengyang Hou0Jingchun Shi1Chengyuan Lin2Lin Zhu3Zhaoxiang Bian4Centre for Chinese Herbal Medicine Drug Development, Hong Kong Baptist University, Hong Kong 999077, PR ChinaCentre for Chinese Herbal Medicine Drug Development, Hong Kong Baptist University, Hong Kong 999077, PR ChinaCentre for Chinese Herbal Medicine Drug Development, Hong Kong Baptist University, Hong Kong 999077, PR ChinaCentre for Chinese Herbal Medicine Drug Development, Hong Kong Baptist University, Hong Kong 999077, PR China; School of Chinese Medicine, Hong Kong Baptist University, Hong Kong 999077, PR China; Corresponding authors at: Centre for Chinese Herbal Medicine Drug Development, Hong Kong Baptist University, Hong Kong 999077, PR China (L. Zhu).Centre for Chinese Herbal Medicine Drug Development, Hong Kong Baptist University, Hong Kong 999077, PR China; School of Chinese Medicine, Hong Kong Baptist University, Hong Kong 999077, PR China; Corresponding authors at: Centre for Chinese Herbal Medicine Drug Development, Hong Kong Baptist University, Hong Kong 999077, PR China (L. Zhu).For the valorization of Chaenomeles speciosa leaves, this study focused on extraction, enrichment, chemical profiling, and investigation of the biological activity of its abundant triterpenoid components. Initially, the total triterpenoids in C. speciosa leaves were extracted by ultrasonic-assisted extraction (UAE) method, with the extraction process optimized through response surface methodology (RSM). Under the optimal conditions of extraction solvent 93 % EtOH, ultrasound power 390 W, extraction time 30 min, extraction temperature 70 °C, liquid-to-solid ratio 25 mL/g, and 2 extraction cycles, the maximum total triterpenoids yield (TTY) reached 36.77 ± 0.40 mg/g. The total triterpenoids in the crude extract were subsequently enriched by X-5 resin column chromatography, resulting in a fourfold increase in purity, reaching 73.27 ± 0.84 %. Thirteen compounds in the triterpenoid-rich fraction (TRF) were identified through UPLC–QTOF-MS/MS, and five major triterpenoids (oleanolic acid, ursolic acid, betulinic acid, maslinic acid, and pomolic acid) were simultaneously quantified by HPLC–QQQ-MS. Furthermore, TRF demonstrated a notable amelioration against dextran sodium sulfate (DSS)-induced ulcerative colitis in mice, indicating its promise as a potent intervention for this condition. In summary, this study will contribute to enhancing the utilization efficiency of Chaenomeles speciosa leaves.http://www.sciencedirect.com/science/article/pii/S1350417724003857Chaenomeles speciosa leavesTriterpenoidsUltrasound-assisted extractionEnrichmentUPLC-QTOF-MS/MS, HPLC-QQQ-MS, Ulcerative colitis |
| spellingShingle | Mengyang Hou Jingchun Shi Chengyuan Lin Lin Zhu Zhaoxiang Bian Ultrasound-assisted extraction of triterpenoids from Chaenomeles speciosa leaves: Process optimization, adsorptive enrichment, chemical profiling, and protection against ulcerative colitis Ultrasonics Sonochemistry Chaenomeles speciosa leaves Triterpenoids Ultrasound-assisted extraction Enrichment UPLC-QTOF-MS/MS, HPLC-QQQ-MS, Ulcerative colitis |
| title | Ultrasound-assisted extraction of triterpenoids from Chaenomeles speciosa leaves: Process optimization, adsorptive enrichment, chemical profiling, and protection against ulcerative colitis |
| title_full | Ultrasound-assisted extraction of triterpenoids from Chaenomeles speciosa leaves: Process optimization, adsorptive enrichment, chemical profiling, and protection against ulcerative colitis |
| title_fullStr | Ultrasound-assisted extraction of triterpenoids from Chaenomeles speciosa leaves: Process optimization, adsorptive enrichment, chemical profiling, and protection against ulcerative colitis |
| title_full_unstemmed | Ultrasound-assisted extraction of triterpenoids from Chaenomeles speciosa leaves: Process optimization, adsorptive enrichment, chemical profiling, and protection against ulcerative colitis |
| title_short | Ultrasound-assisted extraction of triterpenoids from Chaenomeles speciosa leaves: Process optimization, adsorptive enrichment, chemical profiling, and protection against ulcerative colitis |
| title_sort | ultrasound assisted extraction of triterpenoids from chaenomeles speciosa leaves process optimization adsorptive enrichment chemical profiling and protection against ulcerative colitis |
| topic | Chaenomeles speciosa leaves Triterpenoids Ultrasound-assisted extraction Enrichment UPLC-QTOF-MS/MS, HPLC-QQQ-MS, Ulcerative colitis |
| url | http://www.sciencedirect.com/science/article/pii/S1350417724003857 |
| work_keys_str_mv | AT mengyanghou ultrasoundassistedextractionoftriterpenoidsfromchaenomelesspeciosaleavesprocessoptimizationadsorptiveenrichmentchemicalprofilingandprotectionagainstulcerativecolitis AT jingchunshi ultrasoundassistedextractionoftriterpenoidsfromchaenomelesspeciosaleavesprocessoptimizationadsorptiveenrichmentchemicalprofilingandprotectionagainstulcerativecolitis AT chengyuanlin ultrasoundassistedextractionoftriterpenoidsfromchaenomelesspeciosaleavesprocessoptimizationadsorptiveenrichmentchemicalprofilingandprotectionagainstulcerativecolitis AT linzhu ultrasoundassistedextractionoftriterpenoidsfromchaenomelesspeciosaleavesprocessoptimizationadsorptiveenrichmentchemicalprofilingandprotectionagainstulcerativecolitis AT zhaoxiangbian ultrasoundassistedextractionoftriterpenoidsfromchaenomelesspeciosaleavesprocessoptimizationadsorptiveenrichmentchemicalprofilingandprotectionagainstulcerativecolitis |