Protocol for generating human cerebral organoids from two-dimensional cultures of pluripotent stem cells bypassing embryoid body aggregation

Protocol for generating human cerebral organoids from two-dimensional cultures of pluripotent stem cells bypassing embryoid body aggregation

Summary: Human cerebral organoids (hCOs) provide an excellent model for the study of human brain development and disease. Here, we present a protocol to obtain hCOs directly from two-dimensional (2D) pluripotent stem cell (PSC) cultures, avoiding cell dissociation and posterior embryoid body (EB) ag...

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Bibliographic Details
Main Authors: Rosa González-Sastre, Raquel Coronel, Patricia Mateos-Martínez, Laura Maeso, Elena Llorente-Beneyto, Alfonso Luque, Berta Anta, Victoria López-Alonso, Isabel Liste
Format: Article
Language:English
Published: Elsevier 2025-03-01
Series:STAR Protocols
Subjects:
Neuroscience
Stem Cells
Organoids
Online Access:http://www.sciencedirect.com/science/article/pii/S266616672500084X
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