BODIPY-Based Ratiometric Fluorescent Probe for Sensing Peroxynitrite in Inflammatory Cells and Tissues

BODIPY-Based Ratiometric Fluorescent Probe for Sensing Peroxynitrite in Inflammatory Cells and Tissues

Peroxynitrite (ONOO<sup>−</sup>) plays an important role in many physiological and pathological processes. Excessive ONOO<sup>−</sup> in cells leads to oxidative stress and inflammation. However, precise monitoring of ONOO<sup>−</sup> levels in specific organelles...

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Bibliographic Details
Main Authors: Qian Wu, Ziwei Hu, Guoyang Zhang, Yulong Jin, Zhuo Wang
Format: Article
Language:English
Published: MDPI AG 2024-12-01
Series:Biosensors
Subjects:
ratiometric probe
peroxynitrite
fluorescence imaging
inflammatory
Online Access:https://www.mdpi.com/2079-6374/14/12/638
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Summary:Peroxynitrite (ONOO<sup>−</sup>) plays an important role in many physiological and pathological processes. Excessive ONOO<sup>−</sup> in cells leads to oxidative stress and inflammation. However, precise monitoring of ONOO<sup>−</sup> levels in specific organelles (e.g., mitochondria) is still lacking and urgently needed. Herein, we rationally designed a mitochondria-targeted ratiometric fluorescent probe, MOBDP-I, for imaging of ONOO<sup>−</sup> in the mitochondria of inflammatory cells and model mice. This probe, MOBDP-I, was synthesized by conjugating a BODIPY fluorophore to a mitochondria-targeting moiety–indole-salt group by a carbon–carbon double bond (C=C). In the presence of ONOO<sup>−</sup>, the C=C bond between the BODIPY backbone and the indole-salt group was oxidized and broken, leading to an 18-fold enhancement of fluorescence at 510 nm, along with a significant fluorescence decrease at 596 nm. The ratiometric response property bestowed the probe with advantages in the precise quantification of ONOO<sup>−</sup> in cells, thus allowing estimation of the extent of inflammation in living cells and mouse models of rheumatoid arthritis, peritonitis, and brain inflammation. MOBDP-I could act as an effective molecular tool to study the relationship between ONOO<sup>−</sup> and the occurrence and development of inflammatory diseases.
ISSN:2079-6374

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