Freshwater-Derived Streptomyces: Prospective Polyvinyl Chloride (PVC) Biodegraders

Polyvinyl chloride (PVC) is widely used in industrial applications, such as construction and clothing, owing to its chemical, physical, and environmental resistance. Owing to the previous characteristics, PVC is the third most consumed plastic worldwide and, consequently, an increasing waste accumul...

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Main Authors: Maria Fernanda Rodríguez-Fonseca, Sonia Ruiz-Balaguera, Manuel Fernando Valero, Jeysson Sánchez-Suárez, Ericsson Coy-Barrera, Luis Eduardo Díaz
Format: Article
Language:English
Published: Wiley 2022-01-01
Series:The Scientific World Journal
Online Access:http://dx.doi.org/10.1155/2022/6420003
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author Maria Fernanda Rodríguez-Fonseca
Sonia Ruiz-Balaguera
Manuel Fernando Valero
Jeysson Sánchez-Suárez
Ericsson Coy-Barrera
Luis Eduardo Díaz
author_facet Maria Fernanda Rodríguez-Fonseca
Sonia Ruiz-Balaguera
Manuel Fernando Valero
Jeysson Sánchez-Suárez
Ericsson Coy-Barrera
Luis Eduardo Díaz
author_sort Maria Fernanda Rodríguez-Fonseca
collection DOAJ
description Polyvinyl chloride (PVC) is widely used in industrial applications, such as construction and clothing, owing to its chemical, physical, and environmental resistance. Owing to the previous characteristics, PVC is the third most consumed plastic worldwide and, consequently, an increasing waste accumulation-related problem. The current study evaluated an in-house collection of 61 Actinobacteria strains for PVC resin biodegradation. Weight loss percentage was measured after the completion of incubation. Thermo-gravimetric analysis was subsequently performed using the PVC incubated with the three strains exhibiting the highest weight loss. GC-MS and ionic exchange chromatography analyses were also performed using the culture media supernatant of these three strains. After incubation, 14 strains had a PVC weight loss percentage higher than 50% in ISP-2 broth. These 14 strains were identified as Streptomyces strains. Strains 208, 250, and 290 showed the highest weight loss percentages (57.6–61.5% range). The thermal stability of PVC after bacterial exposure using these three strains was evaluated, and a modification of the representative degradation stages of nonincubated PVC was observed. Additionally, GC-MS analysis revealed the presence of aromatic compounds in the inoculated culture media, and ionic exchange chromatography showed chloride release in the supernatant. A mathematical relation between culture conditions and PVC weight loss was also found for strains 208 and 290, showing an accuracy up to 97.99%. These results highlight the potential of the freshwater-derived Streptomyces strains as candidates for the PVC biodegradation strategy and constitute the first approach to a waste management control scale-up process.
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spelling doaj-art-bbc69de145c043aea9f09bd53019b07f2025-08-20T03:22:54ZengWileyThe Scientific World Journal1537-744X2022-01-01202210.1155/2022/6420003Freshwater-Derived Streptomyces: Prospective Polyvinyl Chloride (PVC) BiodegradersMaria Fernanda Rodríguez-Fonseca0Sonia Ruiz-Balaguera1Manuel Fernando Valero2Jeysson Sánchez-Suárez3Ericsson Coy-Barrera4Luis Eduardo Díaz5Process Design and ManagementConservation, Bioprospecting, and Sustainable Development GroupEnergy, Materials and Environment GroupEnvironmental Engineering ProgramBioorganic Chemistry LaboratoryBioprospecting Research GroupPolyvinyl chloride (PVC) is widely used in industrial applications, such as construction and clothing, owing to its chemical, physical, and environmental resistance. Owing to the previous characteristics, PVC is the third most consumed plastic worldwide and, consequently, an increasing waste accumulation-related problem. The current study evaluated an in-house collection of 61 Actinobacteria strains for PVC resin biodegradation. Weight loss percentage was measured after the completion of incubation. Thermo-gravimetric analysis was subsequently performed using the PVC incubated with the three strains exhibiting the highest weight loss. GC-MS and ionic exchange chromatography analyses were also performed using the culture media supernatant of these three strains. After incubation, 14 strains had a PVC weight loss percentage higher than 50% in ISP-2 broth. These 14 strains were identified as Streptomyces strains. Strains 208, 250, and 290 showed the highest weight loss percentages (57.6–61.5% range). The thermal stability of PVC after bacterial exposure using these three strains was evaluated, and a modification of the representative degradation stages of nonincubated PVC was observed. Additionally, GC-MS analysis revealed the presence of aromatic compounds in the inoculated culture media, and ionic exchange chromatography showed chloride release in the supernatant. A mathematical relation between culture conditions and PVC weight loss was also found for strains 208 and 290, showing an accuracy up to 97.99%. These results highlight the potential of the freshwater-derived Streptomyces strains as candidates for the PVC biodegradation strategy and constitute the first approach to a waste management control scale-up process.http://dx.doi.org/10.1155/2022/6420003
spellingShingle Maria Fernanda Rodríguez-Fonseca
Sonia Ruiz-Balaguera
Manuel Fernando Valero
Jeysson Sánchez-Suárez
Ericsson Coy-Barrera
Luis Eduardo Díaz
Freshwater-Derived Streptomyces: Prospective Polyvinyl Chloride (PVC) Biodegraders
The Scientific World Journal
title Freshwater-Derived Streptomyces: Prospective Polyvinyl Chloride (PVC) Biodegraders
title_full Freshwater-Derived Streptomyces: Prospective Polyvinyl Chloride (PVC) Biodegraders
title_fullStr Freshwater-Derived Streptomyces: Prospective Polyvinyl Chloride (PVC) Biodegraders
title_full_unstemmed Freshwater-Derived Streptomyces: Prospective Polyvinyl Chloride (PVC) Biodegraders
title_short Freshwater-Derived Streptomyces: Prospective Polyvinyl Chloride (PVC) Biodegraders
title_sort freshwater derived streptomyces prospective polyvinyl chloride pvc biodegraders
url http://dx.doi.org/10.1155/2022/6420003
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