Modified Activated Carbon: A Supporting Material for Improving Clostridium beijerinckii TISTR1461 Immobilized Fermentation

This study aimed to investigate the effect of activated carbon (AC) as an immobilization material in acetone-butanol-ethanol fermentation. The AC surface was modified with different physical (orbital shaking and refluxing) and chemical (nitric acid, sodium hydroxide and, (3-aminopropyl)triethoxysila...

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Main Authors: Piyawat Chinwatpaiboon, Akarin Boonsombuti, Thanyalak Chaisuwan, Ancharida Savarajara, Apanee Luengnaruemitchai
Format: Article
Language:English
Published: Wiley 2023-01-01
Series:Bioinorganic Chemistry and Applications
Online Access:http://dx.doi.org/10.1155/2023/3600404
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author Piyawat Chinwatpaiboon
Akarin Boonsombuti
Thanyalak Chaisuwan
Ancharida Savarajara
Apanee Luengnaruemitchai
author_facet Piyawat Chinwatpaiboon
Akarin Boonsombuti
Thanyalak Chaisuwan
Ancharida Savarajara
Apanee Luengnaruemitchai
author_sort Piyawat Chinwatpaiboon
collection DOAJ
description This study aimed to investigate the effect of activated carbon (AC) as an immobilization material in acetone-butanol-ethanol fermentation. The AC surface was modified with different physical (orbital shaking and refluxing) and chemical (nitric acid, sodium hydroxide and, (3-aminopropyl)triethoxysilane (APTES)) treatments to enhance the biobutanol production by Clostridium beijerinckii TISTR1461. The effect of surface modification on AC was evaluated using Fourier-transform infrared spectroscopy, field emission scanning electron microscopy, surface area analyses, and X-ray photoelectron spectroscopy, while the fermented broth was examined by high-performance liquid chromatography. The chemical functionalization significantly modified the physicochemical properties of the different treated ACs and further enhanced the butanol production. The AC treated with APTES under refluxing provided the best fermentation results at 10.93 g/L of butanol, 0.23 g/g of yield, and 0.15 g/L/h of productivity, which were 1.8-, 1.5-, and 3.0-fold higher, respectively, than that in the free-cell fermentation. The obtained dried cell biomass also revealed that the treatment improved the AC surface for cell immobilization. This study demonstrated and emphasized the importance of surface properties to cell immobilization.
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publishDate 2023-01-01
publisher Wiley
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spelling doaj-art-bbbf308068bd4a8a92ea720034a0d37f2025-08-20T03:04:38ZengWileyBioinorganic Chemistry and Applications1687-479X2023-01-01202310.1155/2023/3600404Modified Activated Carbon: A Supporting Material for Improving Clostridium beijerinckii TISTR1461 Immobilized FermentationPiyawat Chinwatpaiboon0Akarin Boonsombuti1Thanyalak Chaisuwan2Ancharida Savarajara3Apanee Luengnaruemitchai4The Petroleum and Petrochemical CollegeDepartment of Materials ScienceThe Petroleum and Petrochemical CollegeDepartment of MicrobiologyThe Petroleum and Petrochemical CollegeThis study aimed to investigate the effect of activated carbon (AC) as an immobilization material in acetone-butanol-ethanol fermentation. The AC surface was modified with different physical (orbital shaking and refluxing) and chemical (nitric acid, sodium hydroxide and, (3-aminopropyl)triethoxysilane (APTES)) treatments to enhance the biobutanol production by Clostridium beijerinckii TISTR1461. The effect of surface modification on AC was evaluated using Fourier-transform infrared spectroscopy, field emission scanning electron microscopy, surface area analyses, and X-ray photoelectron spectroscopy, while the fermented broth was examined by high-performance liquid chromatography. The chemical functionalization significantly modified the physicochemical properties of the different treated ACs and further enhanced the butanol production. The AC treated with APTES under refluxing provided the best fermentation results at 10.93 g/L of butanol, 0.23 g/g of yield, and 0.15 g/L/h of productivity, which were 1.8-, 1.5-, and 3.0-fold higher, respectively, than that in the free-cell fermentation. The obtained dried cell biomass also revealed that the treatment improved the AC surface for cell immobilization. This study demonstrated and emphasized the importance of surface properties to cell immobilization.http://dx.doi.org/10.1155/2023/3600404
spellingShingle Piyawat Chinwatpaiboon
Akarin Boonsombuti
Thanyalak Chaisuwan
Ancharida Savarajara
Apanee Luengnaruemitchai
Modified Activated Carbon: A Supporting Material for Improving Clostridium beijerinckii TISTR1461 Immobilized Fermentation
Bioinorganic Chemistry and Applications
title Modified Activated Carbon: A Supporting Material for Improving Clostridium beijerinckii TISTR1461 Immobilized Fermentation
title_full Modified Activated Carbon: A Supporting Material for Improving Clostridium beijerinckii TISTR1461 Immobilized Fermentation
title_fullStr Modified Activated Carbon: A Supporting Material for Improving Clostridium beijerinckii TISTR1461 Immobilized Fermentation
title_full_unstemmed Modified Activated Carbon: A Supporting Material for Improving Clostridium beijerinckii TISTR1461 Immobilized Fermentation
title_short Modified Activated Carbon: A Supporting Material for Improving Clostridium beijerinckii TISTR1461 Immobilized Fermentation
title_sort modified activated carbon a supporting material for improving clostridium beijerinckii tistr1461 immobilized fermentation
url http://dx.doi.org/10.1155/2023/3600404
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AT thanyalakchaisuwan modifiedactivatedcarbonasupportingmaterialforimprovingclostridiumbeijerinckiitistr1461immobilizedfermentation
AT ancharidasavarajara modifiedactivatedcarbonasupportingmaterialforimprovingclostridiumbeijerinckiitistr1461immobilizedfermentation
AT apaneeluengnaruemitchai modifiedactivatedcarbonasupportingmaterialforimprovingclostridiumbeijerinckiitistr1461immobilizedfermentation