Selection, Identification, and Application of Aptamers against Agaricus bisporus Lectin to Establish an Aptamer-AuNPs Colorimetric Method for Detection of ABL
Agaricus bisporus lectin (ABL), which is one of the antinutritional factors in A. bisporus, is an important allergen and harmful to human health. Due to the shortcomings of the current detection methods, it is extremely urgent to establish a rapid and sensitive detection method for ABL in foods. To...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Journal of Food Quality |
| Online Access: | http://dx.doi.org/10.1155/2020/8821295 |
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| author | Hao Gao Yiling Tian Min Zhang Jianhui Liu Yaowu Yuan Jianxin Tan Aijin Ma |
| author_facet | Hao Gao Yiling Tian Min Zhang Jianhui Liu Yaowu Yuan Jianxin Tan Aijin Ma |
| author_sort | Hao Gao |
| collection | DOAJ |
| description | Agaricus bisporus lectin (ABL), which is one of the antinutritional factors in A. bisporus, is an important allergen and harmful to human health. Due to the shortcomings of the current detection methods, it is extremely urgent to establish a rapid and sensitive detection method for ABL in foods. To isolate the ssDNA aptamer of ABL, 13 rounds of subtractive systematic evolution of ligands by exponential enrichment (SELEX) selection were carried out. As a result, six candidate aptamers were selected and further examined for their binding affinity and specificity by enzyme-linked aptamer method. One aptamer (seq-41) against ABL with a high affinity and specificity was isolated and demonstrated to be the optimal aptamer whose dissociation constant reaches the nanomolar level, Kd = 31.17 ± 0.1070 nM. Based on seq-41, an aptamer-AuNPs colorimetric method was established to detect ABL with a linear range of 0.08∼1.70 μg/mL and the detection limit is 0.062 μg/mL. This study provides a novel aptamer-AuNPs colorimetric method with high sensitivity and specificity for detection of ABL and a novel strategy for development of detection method of fungal or plant allergens. |
| format | Article |
| id | doaj-art-bbbd514c0e304d57a6239e7a25f7463f |
| institution | OA Journals |
| issn | 0146-9428 1745-4557 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Food Quality |
| spelling | doaj-art-bbbd514c0e304d57a6239e7a25f7463f2025-08-20T02:18:42ZengWileyJournal of Food Quality0146-94281745-45572020-01-01202010.1155/2020/88212958821295Selection, Identification, and Application of Aptamers against Agaricus bisporus Lectin to Establish an Aptamer-AuNPs Colorimetric Method for Detection of ABLHao Gao0Yiling Tian1Min Zhang2Jianhui Liu3Yaowu Yuan4Jianxin Tan5Aijin Ma6College of Food Science and Technology, Hebei Agricultural University, No. 289 Lingyusi Street, Baoding 071001, Hebei, ChinaCollege of Food Science and Technology, Hebei Agricultural University, No. 289 Lingyusi Street, Baoding 071001, Hebei, ChinaShijiazhuang Junlebao Dairy Co., Ltd., No. 36 Shitong Road, Shijiazhuang 050221, Hebei, ChinaCollege of Food Science and Technology, Hebei Agricultural University, No. 289 Lingyusi Street, Baoding 071001, Hebei, ChinaCollege of Food Science and Technology, Hebei Agricultural University, No. 289 Lingyusi Street, Baoding 071001, Hebei, ChinaCollege of Food Science and Technology, Hebei Agricultural University, No. 289 Lingyusi Street, Baoding 071001, Hebei, ChinaBeijing Technology and Business University, Beijing, No. 11 Fucheng Road, Beijing 100048, ChinaAgaricus bisporus lectin (ABL), which is one of the antinutritional factors in A. bisporus, is an important allergen and harmful to human health. Due to the shortcomings of the current detection methods, it is extremely urgent to establish a rapid and sensitive detection method for ABL in foods. To isolate the ssDNA aptamer of ABL, 13 rounds of subtractive systematic evolution of ligands by exponential enrichment (SELEX) selection were carried out. As a result, six candidate aptamers were selected and further examined for their binding affinity and specificity by enzyme-linked aptamer method. One aptamer (seq-41) against ABL with a high affinity and specificity was isolated and demonstrated to be the optimal aptamer whose dissociation constant reaches the nanomolar level, Kd = 31.17 ± 0.1070 nM. Based on seq-41, an aptamer-AuNPs colorimetric method was established to detect ABL with a linear range of 0.08∼1.70 μg/mL and the detection limit is 0.062 μg/mL. This study provides a novel aptamer-AuNPs colorimetric method with high sensitivity and specificity for detection of ABL and a novel strategy for development of detection method of fungal or plant allergens.http://dx.doi.org/10.1155/2020/8821295 |
| spellingShingle | Hao Gao Yiling Tian Min Zhang Jianhui Liu Yaowu Yuan Jianxin Tan Aijin Ma Selection, Identification, and Application of Aptamers against Agaricus bisporus Lectin to Establish an Aptamer-AuNPs Colorimetric Method for Detection of ABL Journal of Food Quality |
| title | Selection, Identification, and Application of Aptamers against Agaricus bisporus Lectin to Establish an Aptamer-AuNPs Colorimetric Method for Detection of ABL |
| title_full | Selection, Identification, and Application of Aptamers against Agaricus bisporus Lectin to Establish an Aptamer-AuNPs Colorimetric Method for Detection of ABL |
| title_fullStr | Selection, Identification, and Application of Aptamers against Agaricus bisporus Lectin to Establish an Aptamer-AuNPs Colorimetric Method for Detection of ABL |
| title_full_unstemmed | Selection, Identification, and Application of Aptamers against Agaricus bisporus Lectin to Establish an Aptamer-AuNPs Colorimetric Method for Detection of ABL |
| title_short | Selection, Identification, and Application of Aptamers against Agaricus bisporus Lectin to Establish an Aptamer-AuNPs Colorimetric Method for Detection of ABL |
| title_sort | selection identification and application of aptamers against agaricus bisporus lectin to establish an aptamer aunps colorimetric method for detection of abl |
| url | http://dx.doi.org/10.1155/2020/8821295 |
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