Effects of Uniform or Multi-Gradient Equilibration During Vitrification on Mitochondrial Damage in Mouse Oocytes
Background: Oocyte cryopreservation is a crucial technique in contemporary society since it provides a means for future fertility preservation at a time when delayed marriage and pregnancy are common. However, oocyte cryopreservation remains challenging because of the inherent vul...
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| Format: | Article |
| Language: | English |
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IMR Press
2025-03-01
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| Series: | Clinical and Experimental Obstetrics & Gynecology |
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| Online Access: | https://www.imrpress.com/journal/CEOG/52/3/10.31083/CEOG27924 |
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| author | Eun Hee Yu Jung Ran Cho Ha Eun Jung Hyun Joo Lee Jong Kil Joo |
| author_facet | Eun Hee Yu Jung Ran Cho Ha Eun Jung Hyun Joo Lee Jong Kil Joo |
| author_sort | Eun Hee Yu |
| collection | DOAJ |
| description | Background: Oocyte cryopreservation is a crucial technique in contemporary society since it provides a means for future fertility preservation at a time when delayed marriage and pregnancy are common. However, oocyte cryopreservation remains challenging because of the inherent vulnerability of oocytes becoming damaged during freezing and thawing. This study investigated the effects of uniform versus multi-gradient equilibration during vitrification on mitochondrial integrity and distribution in mouse oocytes. Methods: We compared a conventional uniform equilibration method involving a 10-minute exposure to cryoprotective agents using a multi-gradient equilibration method that reduced the exposure time to 2.5 minutes. The survival rates of the vitrified oocytes and the mitochondrial fluorescence intensity and distribution were assessed using confocal microscopy. Results: The survival rates were not significantly different between the two methods. However, the multi-gradient equilibration method presented a higher mitochondrial fluorescence intensity and more uniform distribution, indicating better preservation of mitochondrial function. Conclusion: These findings suggest that the multi-gradient equilibration method may offer a viable alternative to conventional vitrification that can reduce mitochondrial damage and potentially improve oocyte quality post-thawing. |
| format | Article |
| id | doaj-art-bbb6a6430ae146bd8cf54826f80bd1e2 |
| institution | DOAJ |
| issn | 0390-6663 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | IMR Press |
| record_format | Article |
| series | Clinical and Experimental Obstetrics & Gynecology |
| spelling | doaj-art-bbb6a6430ae146bd8cf54826f80bd1e22025-08-20T02:48:28ZengIMR PressClinical and Experimental Obstetrics & Gynecology0390-66632025-03-015232792410.31083/CEOG27924S0390-6663(25)02560-6Effects of Uniform or Multi-Gradient Equilibration During Vitrification on Mitochondrial Damage in Mouse OocytesEun Hee Yu0Jung Ran Cho1Ha Eun Jung2Hyun Joo Lee3Jong Kil Joo4Department of Obstetrics and Gynecology, Pusan National University School of Medicine, 49241 Busan, Republic of KoreaDepartment of Obstetrics and Gynecology, Pusan National University Hospital Infertility Center, Pusan National University Hospital, 49241 Busan, Republic of KoreaDepartment of Obstetrics and Gynecology, Pusan National University School of Medicine, 49241 Busan, Republic of KoreaDepartment of Obstetrics and Gynecology, Pusan National University School of Medicine, 49241 Busan, Republic of KoreaDepartment of Obstetrics and Gynecology, Pusan National University School of Medicine, 49241 Busan, Republic of KoreaBackground: Oocyte cryopreservation is a crucial technique in contemporary society since it provides a means for future fertility preservation at a time when delayed marriage and pregnancy are common. However, oocyte cryopreservation remains challenging because of the inherent vulnerability of oocytes becoming damaged during freezing and thawing. This study investigated the effects of uniform versus multi-gradient equilibration during vitrification on mitochondrial integrity and distribution in mouse oocytes. Methods: We compared a conventional uniform equilibration method involving a 10-minute exposure to cryoprotective agents using a multi-gradient equilibration method that reduced the exposure time to 2.5 minutes. The survival rates of the vitrified oocytes and the mitochondrial fluorescence intensity and distribution were assessed using confocal microscopy. Results: The survival rates were not significantly different between the two methods. However, the multi-gradient equilibration method presented a higher mitochondrial fluorescence intensity and more uniform distribution, indicating better preservation of mitochondrial function. Conclusion: These findings suggest that the multi-gradient equilibration method may offer a viable alternative to conventional vitrification that can reduce mitochondrial damage and potentially improve oocyte quality post-thawing.https://www.imrpress.com/journal/CEOG/52/3/10.31083/CEOG27924oocyte cryopreservationmulti-gradient equilibration vitrificationmitochondria |
| spellingShingle | Eun Hee Yu Jung Ran Cho Ha Eun Jung Hyun Joo Lee Jong Kil Joo Effects of Uniform or Multi-Gradient Equilibration During Vitrification on Mitochondrial Damage in Mouse Oocytes Clinical and Experimental Obstetrics & Gynecology oocyte cryopreservation multi-gradient equilibration vitrification mitochondria |
| title | Effects of Uniform or Multi-Gradient Equilibration During Vitrification on Mitochondrial Damage in Mouse Oocytes |
| title_full | Effects of Uniform or Multi-Gradient Equilibration During Vitrification on Mitochondrial Damage in Mouse Oocytes |
| title_fullStr | Effects of Uniform or Multi-Gradient Equilibration During Vitrification on Mitochondrial Damage in Mouse Oocytes |
| title_full_unstemmed | Effects of Uniform or Multi-Gradient Equilibration During Vitrification on Mitochondrial Damage in Mouse Oocytes |
| title_short | Effects of Uniform or Multi-Gradient Equilibration During Vitrification on Mitochondrial Damage in Mouse Oocytes |
| title_sort | effects of uniform or multi gradient equilibration during vitrification on mitochondrial damage in mouse oocytes |
| topic | oocyte cryopreservation multi-gradient equilibration vitrification mitochondria |
| url | https://www.imrpress.com/journal/CEOG/52/3/10.31083/CEOG27924 |
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