Association of Autoimmune Thyroid Disease with Anti-GAD Antibody ELISA Test Positivity and Risk for Insulin Deficiency in Slowly Progressive Type 1 Diabetes
The presence of antiglutamic acid decarboxylase antibody (GADA) is required for the diagnosis of slowly progressive type 1 diabetes (SPT1D). We examined the factors influencing GADA determination by radioimmunoassay (GADA-RIA) and by enzyme-linked immunosorbent assay (GADA-ELISA). Sixty patients wit...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2018/1847430 |
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| author | Masahito Katahira Hidetada Ogata Takahiro Ito Tsutomu Miwata Megumi Goto Shizuka Nakamura Hiromi Takashima |
| author_facet | Masahito Katahira Hidetada Ogata Takahiro Ito Tsutomu Miwata Megumi Goto Shizuka Nakamura Hiromi Takashima |
| author_sort | Masahito Katahira |
| collection | DOAJ |
| description | The presence of antiglutamic acid decarboxylase antibody (GADA) is required for the diagnosis of slowly progressive type 1 diabetes (SPT1D). We examined the factors influencing GADA determination by radioimmunoassay (GADA-RIA) and by enzyme-linked immunosorbent assay (GADA-ELISA). Sixty patients with SPT1D and 154 patients with type 2 diabetes were examined by both GADA-RIA and GADA-ELISA and for the presence of autoimmune thyroid disease (AITD). We compared the clinical characteristics of these patients based on the positivity or negativity of GADA-RIA and GADA-ELISA, and the existence or nonexistence of AITD. Thirty of 60 (50.0%) GADA-RIA-positive patients were GADA-ELISA negative, whereas none of the 154 GADA-RIA-negative patients were GADA-ELISA positive. Concomitant AITD was significantly less in patients with GADA-RIA and without GADA-ELISA and was significantly more in patients with GADA-RIA and GADA-ELISA. In GADA-RIA-positive patients, there was no significant difference in the GADA-RIA titer among the GADA-ELISA-negative patients with and without AITD, and the GADA-ELISA-positive patients without AITD; whereas the frequency of insulin deficiency was significantly higher in the patients with AITD and/or GADA-ELISA than in those without AITD and GADA-ELISA. Examination of GADA-ELISA and AITD in GADA-RIA-positive patients might be useful in predicting insulin deficiency in these patients. |
| format | Article |
| id | doaj-art-bbb29aef95b342359d10dc18e9a25d6c |
| institution | Kabale University |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-bbb29aef95b342359d10dc18e9a25d6c2025-02-03T01:12:41ZengWileyJournal of Diabetes Research2314-67452314-67532018-01-01201810.1155/2018/18474301847430Association of Autoimmune Thyroid Disease with Anti-GAD Antibody ELISA Test Positivity and Risk for Insulin Deficiency in Slowly Progressive Type 1 DiabetesMasahito Katahira0Hidetada Ogata1Takahiro Ito2Tsutomu Miwata3Megumi Goto4Shizuka Nakamura5Hiromi Takashima6Aichi Prefectural University School of Nursing and Health, Nagoya 463-8502, JapanDepartment of Endocrinology and Diabetes, Ichinomiya Municipal Hospital, Ichinomiya 491-8558, JapanDepartment of Endocrinology and Diabetes, Ichinomiya Municipal Hospital, Ichinomiya 491-8558, JapanDepartment of Endocrinology and Diabetes, Ichinomiya Municipal Hospital, Ichinomiya 491-8558, JapanDepartment of Endocrinology and Diabetes, Ichinomiya Municipal Hospital, Ichinomiya 491-8558, JapanDepartment of Endocrinology and Diabetes, Ichinomiya Municipal Hospital, Ichinomiya 491-8558, JapanDepartment of Endocrinology and Diabetes, Ichinomiya Municipal Hospital, Ichinomiya 491-8558, JapanThe presence of antiglutamic acid decarboxylase antibody (GADA) is required for the diagnosis of slowly progressive type 1 diabetes (SPT1D). We examined the factors influencing GADA determination by radioimmunoassay (GADA-RIA) and by enzyme-linked immunosorbent assay (GADA-ELISA). Sixty patients with SPT1D and 154 patients with type 2 diabetes were examined by both GADA-RIA and GADA-ELISA and for the presence of autoimmune thyroid disease (AITD). We compared the clinical characteristics of these patients based on the positivity or negativity of GADA-RIA and GADA-ELISA, and the existence or nonexistence of AITD. Thirty of 60 (50.0%) GADA-RIA-positive patients were GADA-ELISA negative, whereas none of the 154 GADA-RIA-negative patients were GADA-ELISA positive. Concomitant AITD was significantly less in patients with GADA-RIA and without GADA-ELISA and was significantly more in patients with GADA-RIA and GADA-ELISA. In GADA-RIA-positive patients, there was no significant difference in the GADA-RIA titer among the GADA-ELISA-negative patients with and without AITD, and the GADA-ELISA-positive patients without AITD; whereas the frequency of insulin deficiency was significantly higher in the patients with AITD and/or GADA-ELISA than in those without AITD and GADA-ELISA. Examination of GADA-ELISA and AITD in GADA-RIA-positive patients might be useful in predicting insulin deficiency in these patients.http://dx.doi.org/10.1155/2018/1847430 |
| spellingShingle | Masahito Katahira Hidetada Ogata Takahiro Ito Tsutomu Miwata Megumi Goto Shizuka Nakamura Hiromi Takashima Association of Autoimmune Thyroid Disease with Anti-GAD Antibody ELISA Test Positivity and Risk for Insulin Deficiency in Slowly Progressive Type 1 Diabetes Journal of Diabetes Research |
| title | Association of Autoimmune Thyroid Disease with Anti-GAD Antibody ELISA Test Positivity and Risk for Insulin Deficiency in Slowly Progressive Type 1 Diabetes |
| title_full | Association of Autoimmune Thyroid Disease with Anti-GAD Antibody ELISA Test Positivity and Risk for Insulin Deficiency in Slowly Progressive Type 1 Diabetes |
| title_fullStr | Association of Autoimmune Thyroid Disease with Anti-GAD Antibody ELISA Test Positivity and Risk for Insulin Deficiency in Slowly Progressive Type 1 Diabetes |
| title_full_unstemmed | Association of Autoimmune Thyroid Disease with Anti-GAD Antibody ELISA Test Positivity and Risk for Insulin Deficiency in Slowly Progressive Type 1 Diabetes |
| title_short | Association of Autoimmune Thyroid Disease with Anti-GAD Antibody ELISA Test Positivity and Risk for Insulin Deficiency in Slowly Progressive Type 1 Diabetes |
| title_sort | association of autoimmune thyroid disease with anti gad antibody elisa test positivity and risk for insulin deficiency in slowly progressive type 1 diabetes |
| url | http://dx.doi.org/10.1155/2018/1847430 |
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