Establishment of a transgene-free iPS cell line (SDCHi002-A) from a young patient bearing a NPRL3 mutation and suffering from Epilepsy
Epilepsy affects ∼ 65 million people worldwide. In this study, peripheral blood mononuclear cells were isolated from a young patient patient bearing a Nitrogen Perntease Regulator Like 3 Protein (NPRL3) mutation and suffering from Epilepsy verified by clinical and genetic diagnosis. Induced pluripot...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-12-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506124002721 |
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| author | Song Su Ying Ren Hongwei Zhang Yi Liu Yong Liu Zilong Li Tong Zhang Fang Fang |
| author_facet | Song Su Ying Ren Hongwei Zhang Yi Liu Yong Liu Zilong Li Tong Zhang Fang Fang |
| author_sort | Song Su |
| collection | DOAJ |
| description | Epilepsy affects ∼ 65 million people worldwide. In this study, peripheral blood mononuclear cells were isolated from a young patient patient bearing a Nitrogen Perntease Regulator Like 3 Protein (NPRL3) mutation and suffering from Epilepsy verified by clinical and genetic diagnosis. Induced pluripotent stem cells (iPSCs) were established by a non-integrative method, using plasmids carrying OCT4, SOX2, KLF4, BCL-XL and C-MYC. The established iPSCs presented typical pluripotent cells morphology, normal karyotype, and potential to differentiate into three germ layers. Our approach offers a useful model to explore pathogenesis and therapy of Epilepsy. |
| format | Article |
| id | doaj-art-bbaf87bfe7f846e88840eb9ddc78b82a |
| institution | DOAJ |
| issn | 1873-5061 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj-art-bbaf87bfe7f846e88840eb9ddc78b82a2025-08-20T02:49:53ZengElsevierStem Cell Research1873-50612024-12-018110357410.1016/j.scr.2024.103574Establishment of a transgene-free iPS cell line (SDCHi002-A) from a young patient bearing a NPRL3 mutation and suffering from EpilepsySong Su0Ying Ren1Hongwei Zhang2Yi Liu3Yong Liu4Zilong Li5Tong Zhang6Fang Fang7Epilepsy Center, Children’s Hospital Affiliated to Shandong University, Jinan, Shandong 250022, China; Epilepsy Center, Jinan Children’s Hospital, Jinan, Shandong 250022, ChinaEpilepsy Center, Children’s Hospital Affiliated to Shandong University, Jinan, Shandong 250022, China; Epilepsy Center, Jinan Children’s Hospital, Jinan, Shandong 250022, ChinaEpilepsy Center, Children’s Hospital Affiliated to Shandong University, Jinan, Shandong 250022, China; Epilepsy Center, Jinan Children’s Hospital, Jinan, Shandong 250022, ChinaEpilepsy Center, Children’s Hospital Affiliated to Shandong University, Jinan, Shandong 250022, China; Epilepsy Center, Jinan Children’s Hospital, Jinan, Shandong 250022, ChinaEpilepsy Center, Children’s Hospital Affiliated to Shandong University, Jinan, Shandong 250022, China; Epilepsy Center, Jinan Children’s Hospital, Jinan, Shandong 250022, ChinaEpilepsy Center, Children’s Hospital Affiliated to Shandong University, Jinan, Shandong 250022, China; Epilepsy Center, Jinan Children’s Hospital, Jinan, Shandong 250022, ChinaEpilepsy Center, Children’s Hospital Affiliated to Shandong University, Jinan, Shandong 250022, China; Epilepsy Center, Jinan Children’s Hospital, Jinan, Shandong 250022, ChinaDepartment of Neurology, Beijing Children’s Hospital, National Center for Children’s Health, Capital Medical University, Beijing 100045, China; Corresponding author.Epilepsy affects ∼ 65 million people worldwide. In this study, peripheral blood mononuclear cells were isolated from a young patient patient bearing a Nitrogen Perntease Regulator Like 3 Protein (NPRL3) mutation and suffering from Epilepsy verified by clinical and genetic diagnosis. Induced pluripotent stem cells (iPSCs) were established by a non-integrative method, using plasmids carrying OCT4, SOX2, KLF4, BCL-XL and C-MYC. The established iPSCs presented typical pluripotent cells morphology, normal karyotype, and potential to differentiate into three germ layers. Our approach offers a useful model to explore pathogenesis and therapy of Epilepsy.http://www.sciencedirect.com/science/article/pii/S1873506124002721 |
| spellingShingle | Song Su Ying Ren Hongwei Zhang Yi Liu Yong Liu Zilong Li Tong Zhang Fang Fang Establishment of a transgene-free iPS cell line (SDCHi002-A) from a young patient bearing a NPRL3 mutation and suffering from Epilepsy Stem Cell Research |
| title | Establishment of a transgene-free iPS cell line (SDCHi002-A) from a young patient bearing a NPRL3 mutation and suffering from Epilepsy |
| title_full | Establishment of a transgene-free iPS cell line (SDCHi002-A) from a young patient bearing a NPRL3 mutation and suffering from Epilepsy |
| title_fullStr | Establishment of a transgene-free iPS cell line (SDCHi002-A) from a young patient bearing a NPRL3 mutation and suffering from Epilepsy |
| title_full_unstemmed | Establishment of a transgene-free iPS cell line (SDCHi002-A) from a young patient bearing a NPRL3 mutation and suffering from Epilepsy |
| title_short | Establishment of a transgene-free iPS cell line (SDCHi002-A) from a young patient bearing a NPRL3 mutation and suffering from Epilepsy |
| title_sort | establishment of a transgene free ips cell line sdchi002 a from a young patient bearing a nprl3 mutation and suffering from epilepsy |
| url | http://www.sciencedirect.com/science/article/pii/S1873506124002721 |
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