Mesenchymal stem cell-derived exosomes ameliorate diabetic kidney disease through NOD2 signaling pathway
Background and aims Diabetic kidney disease (DKD) is one of the most common complications of diabetes. It is reported that mesenchymal stem cells (MSCs) derived exosomes (MSCs-Exo) may have great clinical application potential for the treatment of DKD, but the underlying mechanism has not been illus...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Renal Failure |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/0886022X.2024.2381597 |
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| author | Yinghui Wang Donglin Lu Shasha Lv Xiangchun Liu Gang Liu |
| author_facet | Yinghui Wang Donglin Lu Shasha Lv Xiangchun Liu Gang Liu |
| author_sort | Yinghui Wang |
| collection | DOAJ |
| description | Background and aims Diabetic kidney disease (DKD) is one of the most common complications of diabetes. It is reported that mesenchymal stem cells (MSCs) derived exosomes (MSCs-Exo) may have great clinical application potential for the treatment of DKD, but the underlying mechanism has not been illustrated. To clarify the effect of MSC-Exo on NOD2 signaling pathway in podocytes under high glucose (HG) and DKD, we conduct this study.Methods We co-cultured podocytes and MSCs-Exo under 30 mM HG and injected MSCs-Exo into DKD mice, then we detected the NOD2 signaling pathway by western blot, qRT-PCT, immunofluorescence, transmission electron microscopy and immunohistochemistry both in vitro and in vivo.Results In vitro, HG lead to the apoptosis, increased the ROS level and activated the NOD2 signaling pathway in podocytes, while MSCs-Exo protected podocytes from injury reduced the expression of inflammatory factors including TNF-α, IL-6, IL-1β, and IL-18 and alleviated the inflammatory response, inhibited the activation of NOD2 signaling pathway and the expression of it’s downstream protein p-P65, p-RIP2, prevented apoptosis, increased cell viability in podocytes caused by HG. In vivo, MSCs-Exo alleviated renal injury in DKD mice, protected renal function, decreased urinary albumin excretion and inhibited the activation of NOD2 signaling pathway as well as the inflammation in renal tissue.Conclusion MSCs-Exo protected the podocytes and DKD mice from inflammation by mediating NOD2 pathway, MSCs-Exo may provide a new target for the treatment of DKD. |
| format | Article |
| id | doaj-art-bbaa0127c33c4c44864f8e644bb7d43f |
| institution | OA Journals |
| issn | 0886-022X 1525-6049 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Renal Failure |
| spelling | doaj-art-bbaa0127c33c4c44864f8e644bb7d43f2025-08-20T02:29:56ZengTaylor & Francis GroupRenal Failure0886-022X1525-60492024-12-0146210.1080/0886022X.2024.2381597Mesenchymal stem cell-derived exosomes ameliorate diabetic kidney disease through NOD2 signaling pathwayYinghui Wang0Donglin Lu1Shasha Lv2Xiangchun Liu3Gang Liu4Department of Nephrology, Multidisciplinary Innovation Center for Nephrology, The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, ChinaDepartment of Nephrology, Multidisciplinary Innovation Center for Nephrology, The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, ChinaDepartment of Nephrology, Multidisciplinary Innovation Center for Nephrology, The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, ChinaDepartment of Nephrology, Multidisciplinary Innovation Center for Nephrology, The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, ChinaDepartment of Nephrology, Multidisciplinary Innovation Center for Nephrology, The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, ChinaBackground and aims Diabetic kidney disease (DKD) is one of the most common complications of diabetes. It is reported that mesenchymal stem cells (MSCs) derived exosomes (MSCs-Exo) may have great clinical application potential for the treatment of DKD, but the underlying mechanism has not been illustrated. To clarify the effect of MSC-Exo on NOD2 signaling pathway in podocytes under high glucose (HG) and DKD, we conduct this study.Methods We co-cultured podocytes and MSCs-Exo under 30 mM HG and injected MSCs-Exo into DKD mice, then we detected the NOD2 signaling pathway by western blot, qRT-PCT, immunofluorescence, transmission electron microscopy and immunohistochemistry both in vitro and in vivo.Results In vitro, HG lead to the apoptosis, increased the ROS level and activated the NOD2 signaling pathway in podocytes, while MSCs-Exo protected podocytes from injury reduced the expression of inflammatory factors including TNF-α, IL-6, IL-1β, and IL-18 and alleviated the inflammatory response, inhibited the activation of NOD2 signaling pathway and the expression of it’s downstream protein p-P65, p-RIP2, prevented apoptosis, increased cell viability in podocytes caused by HG. In vivo, MSCs-Exo alleviated renal injury in DKD mice, protected renal function, decreased urinary albumin excretion and inhibited the activation of NOD2 signaling pathway as well as the inflammation in renal tissue.Conclusion MSCs-Exo protected the podocytes and DKD mice from inflammation by mediating NOD2 pathway, MSCs-Exo may provide a new target for the treatment of DKD.https://www.tandfonline.com/doi/10.1080/0886022X.2024.2381597Diabetic kidney diseaseexosomesmesenchymal stem cellsNOD2 |
| spellingShingle | Yinghui Wang Donglin Lu Shasha Lv Xiangchun Liu Gang Liu Mesenchymal stem cell-derived exosomes ameliorate diabetic kidney disease through NOD2 signaling pathway Renal Failure Diabetic kidney disease exosomes mesenchymal stem cells NOD2 |
| title | Mesenchymal stem cell-derived exosomes ameliorate diabetic kidney disease through NOD2 signaling pathway |
| title_full | Mesenchymal stem cell-derived exosomes ameliorate diabetic kidney disease through NOD2 signaling pathway |
| title_fullStr | Mesenchymal stem cell-derived exosomes ameliorate diabetic kidney disease through NOD2 signaling pathway |
| title_full_unstemmed | Mesenchymal stem cell-derived exosomes ameliorate diabetic kidney disease through NOD2 signaling pathway |
| title_short | Mesenchymal stem cell-derived exosomes ameliorate diabetic kidney disease through NOD2 signaling pathway |
| title_sort | mesenchymal stem cell derived exosomes ameliorate diabetic kidney disease through nod2 signaling pathway |
| topic | Diabetic kidney disease exosomes mesenchymal stem cells NOD2 |
| url | https://www.tandfonline.com/doi/10.1080/0886022X.2024.2381597 |
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